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Rapid Progesterone Actions on Bovine Lens Epithelial Cells


, : Rapid Progesterone Actions on Bovine Lens Epithelial Cells. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 3540

Purpose: Membrane steroid binding protein, which we identified in lens epithelial cells, is hypothesized to mediate a rapid effect of progesterone on lens calcium flux. The present result tests this hypothesis. Methods: We studied the effect of progesterone on Ca2+ signaling in in situ lens epithelial cells loaded with Fura-2 and fluorescence imaging system to evaluate the short-term effect of progesterone. The effect of progesterone on the calcium channel receptors was studied using Mn quenching technique. Results: Progesterone (10-100uM) induced a transient increase in (Ca2+)i within seconds, which is due to Ca2+ influx from the extracellular milieu, as shown by the effects of calcium chelator EGTA . Progesterone has no significant effect on the store-mediated release in the absence of extracellular Ca2+. Progesterone inhibited the thapsigargin-induced sustained (Ca2+)i elevation. It appears that progesterone reduces thapsigargin mediated calcium influx. A Mn2+-induced decline of fluorescent intensity at 360nm excitation was accelerated. However, in some cells, there was a transient decline in the rate of Mn2+ quench rate. Conclusion: This is the first report of a nongenomic rapid effects of a steroid hormone on lens cells and specifically on the intracellular concentration of calcium in lens epithelial cells. These data indicate a novel mode of direct action of progesterone on lens epithelial cells, which is not mediated through the classical nuclear receptor response, but likely through plasma membrane receptors.

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