geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Regulation of neuronal excitability by the inebriated encoded neurotransmitter transporter


, : Regulation of neuronal excitability by the inebriated encoded neurotransmitter transporter. Society for Neuroscience Abstracts 26(1-2): Abstract No -128 1

The Drosophila inebriated (ine) gene encodes a member of the Na+/Cl- -dependent neurotransmitter transporter family. Mutations in the ine gene cause increased neuronal excitability, as was indicated by an increased rate of onset of long term facilitation in the larval motor neuron and a "down-turned wings and indented dorsal thorax" morphological defect in a Shaker mutant background. In this study we used the UAS/GAL4 system to achieve ectopic expression of the Ine transporer and analyze the effects on neuronal excitability. The ine gene expresses two isoforms of the transporter: the long form contains 300 amino acids at the N terminal intracellular domain but is otherwise identical to the short form. We found that re-introduction of either isoform was able to rescue the ine mutant phenotypes, although the long form seems to function more efficiently. We also found that the mutant phenotypes can be rescued by expression of the Ine transporter in either glial cells or neurons. More interestingly, ectopic expression of the Ine transporter on a wildtype background reduces the rate of onset of long term facilitation. This result further confirms that the level of the Ine transporter can regulate neuronal excitability. Establishment and characterization of such an overexpression system will be very useful in dissecting the molecular mechanisms of excitability regulation by neurotransmitter transporters. We are currently in the process of investigating the effect of overexpression of the ine gene on various ion channels mutations such as Shaker, eag, and para.

US$29.90


Other references

Zadik, Y.; Yarom, N.; Elad, S., 2014: Medicolegal considerations in bisphosphonate-related osteonecrosis of the jaw. Oral Diseases 19(6): 628-628

Mamay Sergius H., 1968: Russellites, new genus, a problematical plant from the lower Permian of Texas. Russellites taeniata n. sp., Noeggerathiales?, Belle Plains formation, Baylor County.

Agafonov L.I.; Mazepa V.S., 2001: Ob River runoff and summer air temperature in northern West Siberia. Izvestiya Akademii Nauk, Rossiyskaya Akademiya Nauk, Seriya Geograficheskaya 2001(1): 82-90

Chen, Z.; Xu, H.; Haimano, S.; Li, X.; Li, X-Min., 2005: Quetiapine and venlafaxine synergically regulate heme oxygenase-2 protein expression in the hippocampus of stressed rats. HO-2 is a constitutive isoform of heme oxygenase (HO), a microsomal enzyme that catalyzes the cleavage of the heme ring to form ferrous iron, carbon monoxide, and biliverdin. In contrast to HO-1, which is inducible, HO-2 is not responsive to stimu...

Munir Ahmad; Abdullah M.N.rdin, 2003: Development of an interactive embeddable geographic information system (E-GIS) for soil erosion prediction. Erosion is a critical and dynamic aspect of any water resources project. A computer model was developed for simulating the dynamic aspects of erosion. The computer model was developed using Borland Delphi and ESRI MapObject components. The model i...

Radman M., 1971: Phenomenological theory of repair kinetics of uv irradiated lambda phage. Virology: 422-426

Louisot P.; Lebre D.; Pradal M B.; Gresle J.; Got R., 1970: Glyco protein biosynthesis part 14 role of cytoplasmic sites in the embryonic and neoplastic cells of chicks in culture in suspension. Canadian Journal of Biochemistry 48(9): 1082-1086

Oberg, G.; Nordlund, E.; Berg, B., 1996: In situ formation of organically bound halogens during decomposition of Norway spruce needles: effects of fertilization. Past years of research indicate that halogenation of organic matter is a general occurrence in soil. As this is a virgin research field, little is known about, for example, the possible relation to the turnover of organic matter, influence of envi...

Mier T.; Navarro H.; Martinez A.; Toriello C., 1984: Immunological identification of entomophthorales by their exoantigens. Revista Latinoamericana de Microbiologia 26(2): 152

Rhoades, J.D.; Bingham, F.T.; Letey, J.; Dedrick, A.R.; Bean, M.; Hoffman, G.J.; Alves, W.J.; Swain, R.V.; Pacheco, P.G.; Et-Al, 1988: Reuse of drainage water for irrigation results of imperial valley california usa study i. hypothesis experimental procedures and cropping results. An irrigation/cropping management strategy has been developed to facilitate the use of brackish waters for irrigation, with the goal of expanding the available water supply and minimizing the off-site pollution potential of drainange disposal. A f...