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Some physiological and biochemical mechanisms of redifferentiation difference among three calli of Anthurium andraeanum


, : Some physiological and biochemical mechanisms of redifferentiation difference among three calli of Anthurium andraeanum. Hortscience 38(5): 760, August

To study physiological and biochemical mechanisms of redifferentiation in anthurium, we compared contents of soluble saccharides and soluble proteins, activities and isozyme zymogram of POD and SOD, and levels of endogenous phytohormones, i.e., ZRs, IAA, ABA, and GA (1+3) of three calli of Anthurium andraeanum 'Jolanba' with different redifferentiation behaviors. High content of soluble proteins favored shoot differentiation, and high content of soluble saccharides enhanced shoot growth. There was apparent positive correlation between activities of POD and SOD and number of shoots, between shoot height and GA(1+3)+ABA or ZRs+ABA, and between rhizogenesis and IAA+ABA or IAA alone. Replacing the in vitro culture media and prolonging culture period increased the growth vigor of the aseptic plantlets due to increased content of endogenous soluble saccharides and a favorable balance of endogenous phytohormes.

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