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Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes


, : Cryopreservation of bull semen shipped overnight and its effect on post-thaw sperm motility, plasma membrane integrity, mitochondrial membrane potential and normal acrosomes. Animal Reproduction Science 126(1-2): 23-31

In the Canadian Animal Genetic Resource Program, bull semen is donated in frozen or fresh (diluted) states. This study was designed to assess the cryopreservation of diluted bull semen shipped at 4°C overnight, and to determine the post-thaw quality of shipped semen using different straw volumes and freezing rates. Semen was collected from four breeding bulls (three ejaculates per bull). Semen was diluted in Tris-citric acid-egg yolk-glycerol (TEYG) extender, cooled to 4°C and frozen as per routine (control semen). After cooling to 4°C, a part of semen was removed and shipped overnight to the research laboratory via express courier (shipped semen). Semen was packaged in 0.25 or 0.5 ml straws and frozen in a programmable freezer using three freezing rates, i.e., -10, -25 or -40°C/min. Control semen was also shipped to the research laboratory. Post-thaw sperm motility characteristics were assessed using CASA, and post-thaw sperm plasma membrane, mitochondrial membrane potential and normal acrosomes were assessed using flow cytometry. Post-thaw sperm quality was greater in shipped semen as compared to control (P<0.001). The shipped semen packaged in 0.25 ml straws had better post-thaw sperm quality than in 0.5 ml straws (P<0.001). Freezing rate had no effect on post-thaw sperm quality. In conclusion, bull semen can be shipped overnight for subsequent cryopreservation and gene banking. Overnight shipping of semen was found advantageous for bull semen cryopreservation. Semen packaging in 0.25 ml straws yielded better post-thaw quality than 0.5 ml straws.

US$19.90

PMID: 21621352

DOI: 10.1016/j.anireprosci.2011.04.018


Other references

Isakova, D.M.; Eliseeva, G.S., 1975: Synthesis of vitamin B groups by Micrococcus freudenreichii K-219 in carbohydrate and hydrocarbon nutrition media. Mikrobiolohichnyi Zhurnal 37(1): 7-10

Shiio, T.; Kitamura, K.; Ohishi, K.; Yugari, Y., 1987: Suppressive effect of lentinan on pulmonary metastases in murine tumors. Lentinan has been shown to have a suppressive effect on the natural pulmonary metastasis of Lewis lung carcinoma, though its effect seems to depend on the timing of the administration. Administration of lentinan after the amputation of tumor was f...

Lenton, L.; Behm, C.; Bygrave, F., 1994: Characterization of the oligomycin-sensitivity properties of the F1F0-ATPase in mitochondria from rats infected with the liver fluke Fasciola hepatica. The F1F0-ATPase activity of liver mitochondria isolated from rats infected with Fasciola hepatica at 3 and 4 weeks post-infection showed a marked loss of sensitivity to oligomycin and to N,N'-dicyclohexylcarbodiimide. A loss of sensitivity to...

Mele P.C.; Mcdonough J.H., 1990: Stimulus control modulates effects of ionizing radiation on operant performance. Society for Neuroscience Abstracts {6(1): 601

Davi, M.V.; Pia, A.; Guarnotta, V.; Pizza, G.; Colao, A.; Faggiano, A.; Albertelli, M.; Arvat, E.; Baldelli, R.; Berruti, A.; Bianchi, A.; Bodei, L.; Botti, G.; Corcione, F.; Delle Fave, G.; D.M.rinis, L.; D.R.sa, G.; D.S.rno, A.; Dicitore, A.; Fazio, N.; Fanciulli, G.; Ferolla, P.; Ferone, D.; Filice, A.; Gallo, M.; Giordano, C.; Giuffrida, D.; Lania, A.; Lastoria, S.; Logoluso, F.; Loli, P.; Malandrino, P.; Manzoni, M.; Marchetti, M.; Martini, C.; Messina, E.; Modica, R.; Motta, C.; Papotti, M, 2016: The treatment of hyperinsulinemic hypoglycaemia in adults: an update. Treatment of hyperinsulinemic hypoglycaemia (HH) is challenging due to the rarity of this condition and the difficulty of differential diagnosis. The aim of this article is to give an overview of the recent literature on the management of adult HH...

Fessenden, M., 2013: Ring cycle. Scientific American 308(6): 74-75

Liang, G.; Ahlqvist, K.; Pannem, R.; Posern, G.; Massoumi, R., 2011: Serum response factor controls CYLD expression via MAPK signaling pathway. Tumor suppressor gene CYLD is a deubiquitinating enzyme which negatively regulates various signaling pathways by removing the lysine 63-linked polyubiquitin chains from several specific substrates. Loss of CYLD in different types of tumors leads t...

Ubeira, F.M.; Iglesias, R., 2000: Monoclonal antibodies in the study of Anisakis simplex. Aspects of the immunobiology of Anisakis simplex are reviewed, with particular attention to recently developed immunodiagnostic tests based on monoclonal antibodies to Anisakis-specific antigens. Topics include the antigenic complexity of Anisakis...

Krappmann, S.; Braus, G.H., 2003: Deletion of Aspergillus nidulans aroC using a novel blaster module that combines ET cloning and marker rescue. Blaster cassettes are of significant value in functional genomics, as they represent tools with which to inactivate duplicated or homologous genes in an individual organism. We have constructed a novel blaster module which allows repeated gene del...

Kim, Y.Bae.; Shin, K.Ho.; Seong, J.; Roh, J.Kyung.; Kim, G.Eon.; Hahn, S.Bong.; Suh, C.Ok., 2007: Clinical significance of margin status in postoperative radiotherapy for extremity and truncal soft-tissue sarcoma. Purpose: To evaluate whether adjuvant radiotherapy (RT) in extremity and truncal soft-tissue sarcoma (STS) patients with microscopically positive or close margins after excision can achieve comparable local control to that of excision with negativ...