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Mutation rates in scots pine (Pinus sylvestris L.) from the Chernobyl exclusion zone evaluated with amplified fragment-length polymorphisms (AFLPs) and microsatellite markers

, : Mutation rates in scots pine (Pinus sylvestris L.) from the Chernobyl exclusion zone evaluated with amplified fragment-length polymorphisms (AFLPs) and microsatellite markers. Mutation Research 725(1-2): 29-35

Ionizing radiation is a strong mutagenic factor and, accordingly, elevated mutation rates would be expected in plants exposed to high chronic or acute radiation after the Chernobyl accident in 1986. Somatic mutations were analyzed in pines (Pinus sylvestris L.) planted before and after the Chernobyl accident and in control material of the same origin planted in sites with natural radiation. Microsatellites (SSRs) and amplified fragment-length polymorphisms (AFLPs) were investigated. The mutation rates for microsatellites were estimated as 2.8 × 10(-4)-7.1 × 10(-4) per locus for different irradiated tree populations; no mutations were detected in the controls. In the case of AFLPs, the observed mutation rates were 3.74 × 10(-3) -3.99 × 10(-3) and 1.06 × 10(-3) per locus for contaminated and control areas, respectively. Thus a statistically highly significant three-fold increase in number of mutations was found by the use of AFLP markers, indicating that ionizing radiation causes strong DNA damage across the entire genome and that AFLPs may be the appropriate marker system for this kind of analysis.


PMID: 21782970

DOI: 10.1016/j.mrgentox.2011.07.003

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Casares, J.A.; Espinet, P.; Martín-Alvarez, Jé.M.; Santos, Vónica., 2004: Anionic platinum complexes with 2-pyridylphosphines as ligands for rhodium: synthesis of zwitterionic Pt-Rh organometallic compounds. Bimetallic zwitterionic platinum(II)-rhodium(I) complexes of the type [(C(6)F(5))(3)Pt(micro-PPy(n)Ph(3)(-)(n)Rh(CO)(2))] and [(C(6)F(5))(3)Pt(micro-PPy(n)Ph(3)(-)(n)())Rh(diene))] (n = 2, 3; Py = 2-pyridyl) have been prepared. The P end of the br...

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Dijkstra, C., 1952: Persistent excretion of minute quantities of tubercle bacilli after pulmonary inactivation. Nederlands Tijdschrift Voor Geneeskunde 96(12): 690-694

Romijn J.C.; Verkoelen C.F.; Schroeder F.H.; Splinter T.A.W., 1989: Restoration of cell proliferation by exogenous free and acetylated polyamines in alpha difluoromethylornithine dfmo treated prostate cancer cells. Proceedings of the American Association for Cancer Research Annual Meeting 30: 588

Sinha, D.N.; Narain, J.P.; Kyaing, N.Nyo.; Rinchen, S., 2012: WHO framework convention on tobacco control and its implementation in South-East Asia region. The birth of the WHO Framework Convention on Tobacco Control (WHO FCTC) took place in response to the global tobacco epidemic and it became the most important global tobacco control instrument. Duly recognizing tobacco use as an important public h...

Durrer, J.D., 1987: The treatment of left-sided cardiac decompensation, hypertensive crisis and acute heart infarct with sodium nitroprusside. Nederlands Tijdschrift Voor Geneeskunde 131(35): 1512-1515

Watson, J.A.; Auld, J.A.; Spritzer, A.A., 1969: Upper respiratory tract clearance in rats after thoracic irradiation. Radiation Research 37(1): 57-70

Kawano, H.; Sato, T.; Yamada, T.; Matsumoto, T.; Kawaguchi, H.; Kato, S., 2002: Both androgen and estrogen signalings contribute to the maintenance of bone mass in males Analyses of male androgen receptor deficient mice. Journal of Bone & Mineral Research 17(Suppl 1): S365, September

Jeutter Peter W.; Seebacher Robert, 1997: Totes Gebirge In den Karen. International Caver: ages 19-28. 1997.

Ponty, Y.; Plunian, F., 2011: Transition from large-scale to small-scale dynamo. The dynamo equations are solved numerically with a helical forcing corresponding to the Roberts flow. In the fully turbulent regime the flow behaves as a Roberts flow on long time scales, plus turbulent fluctuations at short time scales. The dynam...