geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The L-coding region of the DA strain of Theiler's murine encephalomyelitis virus causes dysfunction and death of myelin-synthesizing cells


, : The L-coding region of the DA strain of Theiler's murine encephalomyelitis virus causes dysfunction and death of myelin-synthesizing cells. Journal of Virology 85(18): 9377-9384

The DA strain and other members of the TO subgroup of Theiler's murine encephalomyelitis virus (TMEV) induce an early transient subclinical neuronal disease followed by a chronic progressive inflammatory demyelination, with persistence of the virus in the central nervous system (CNS) for the life of the mouse. Although TMEV-induced demyelinating disease (TMEV-IDD) is thought to be immune mediated, there is also evidence that supports a role for the virus in directly inducing demyelination. In order to clarify the function of DA virus genes, we generated a transgenic mouse that had tamoxifen-inducible expression of the DA L-coding region in oligodendrocytes (and Schwann cells), a cell type in which the virus is known to persist. Tamoxifen-treated young transgenic mice usually developed an acute progressive fatal paralysis, with abnormalities of the oligodendrocytes and Schwann cells and demyelination, but without significant lymphocytic infiltration; later treatment led to transient weakness with demyelination and persistent expression of the recombined transgene. These findings demonstrate that a high level of expression of DA L can cause the death of myelin-synthesizing cells and death of the mouse, while a lower level of L expression (which can persist) can lead to cellular dysfunction with survival. The results suggest that expression of DA L plays an important role in the pathogenesis of TMEV-IDD. Virus-induced infection and death of oligodendrocytes may play a part in the demyelination of other diseases in which an immune-mediated mechanism has been stressed, including multiple sclerosis.

US$19.90

PMID: 21752920

DOI: 10.1128/JVI.00178-11


Other references

Todd, K.H.; Thomas, C.R., 2016: An Inflection Point in the Evolution of Oncologic Emergency Medicine. Annals of Emergency Medicine 68(6): 712-716

Riaz, A.H.; Tasma, B.E.; Woodman, M.E.; Wooten, R.Mark.; Worth, R.G., 2012: Human platelets efficiently kill IgG-opsonized E. coli. Platelets are known contributors of hemostasis but have recently been shown to be important in inflammation and infectious diseases. Moreover, thrombocytopenia is often observed in patients with sepsis. We previously reported that platelets active...

Nyangito, H.O.; Richardson, J.W.; Mukhebi, A.W.; Mundy, D.S.; Zimmel, P.; Namken, J.; Perry, B.D., 1994: Whole farm economic analysis of East Coast fever immunization strategies in Kilifi District, Kenya. A whole farm simulation model termed the Technology Impact Evaluation System (TIES) was applied for assessing ex-ante financial and economic impacts of immunization of dairy cattle against East Coast fever (ECF) by the infection and treatment meth...

Holle, J.U.; Laudien, M.; Gross, W.L., 2010: Clinical manifestations and treatment of Wegener's granulomatosis. Wegener's granulomatosis (WG) is characterized by granulomatous lesions and vasculitic disease manifestations. Granulomatous lesions are found in the upper and lower respiratory tract (eg, granulomatous sinusitis, orbital masses, and pulmonar...

Zhao, Y.; Zhang, Y.; Cui, X.; Ruan, Y.; Zhang, L., 2015: Association study between single nucleotide polymorphism in AOAH gene and chronic rhinosinusitis in a Chinese population. To replicate the polymorphisms in risk genes of chronic rhinosinusitis in a Chinese Han population. Enrolled in this study were CRS patients with nasal polyps (n = 306, CRSwNP), CRS patients without nasal polyps (n = 332, CRSsNP), and controls (n...

Linder, M.; Brinck, T., 2013: On the method-dependence of transition state asynchronicity in Diels-Alder reactions. This work discusses the dependence of transition state geometries on the choice of quantum chemical optimization method for the extensively studied Diels-Alder reaction. Rather significant differences are observed between post-Hartree-Fock methods...

Sturm, L.; Dawson, D.; Vaughan, R.; Hewett, P.; Hill, A.G.; Graham, J.C.; Maddern, G.J., 2012: Effects of fatigue on surgeon performance and surgical outcomes: a systematic review. Sleep deprivation and disturbances in circadian rhythms generally lead to poor performance, but is there a link in surgery? This review aimed to determine whether fatigue has an impact on surgeon performance or surgical outcomes. Studies were iden...

Stoddart J.A., 1983: Asexual production of planulae in the coral pocillopora damicornis. The reproduction of scleractinian corals through planular larvae has traditionally been viewed as a strictly sexual process, but an electrophoretic study of a ubiquitous Indo-Pacific coral, P. damicornis, shows an exact inheritance of parental gen...

Wucherpfenning, K.; Bretthauer, G., 1962: Fruit acids in apple and pear juice. A chromatographic method of determination of the acids is described. Cider apples contained chiefly malic and some quinic acid; dessert pears contained malic, quinic and tartaric acids; and wine pears contained malic, citric, quinic and tartaric a...

Marks, D.I., 2008: 'Rainy day' stem cell storage for adult patients with acute myeloid leukaemia in first complete remission. Internal Medicine Journal 38(8): 680-1; Author Reply 681