+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Transcriptional activation of pig Galβ1,3GalNAc α2,3-sialyltransferase (pST3Gal I) gene by TGF-β1 in porcine kidney PK-15 cells

, : Transcriptional activation of pig Galβ1,3GalNAc α2,3-sialyltransferase (pST3Gal I) gene by TGF-β1 in porcine kidney PK-15 cells. Biochemical and Biophysical Research Communications 414(1): 159-164

In this study we investigated for the first time the transcriptional regulation of pig Gal?1,3GalNAc ?2,3-sialyltransferase (pST3Gal I) in response to TGF-?1 in porcine kidney PK-15 cells. The pST3Gal I gene was found to span about 9 kb and to be composed of 8 exons including 2 exons in the 5?-untranslated region. RT-PCR analysis indicated that the induction of pST3Gal I by TGF-?1 is regulated at the transcriptional level. Functional analysis of the 5?-flanking region of the pST3Gal I gene revealed the ?1257 to ?976 region functions as the TGF-?1-inducible promoter and that the Smad-binding site at ?12 is crucial for TGF-?1-induced expression of pST3Gal I in PK-15 cells. In addition, the transcriptional activity of pST3Gal I induced by TGF-?1 in PK-15 cells was strongly inhibited by SIS3, which is a specific Smad-3 inhibitor. In summary, our results identified the core promoter region in the pST3Gal I promoter and demonstrated that Smad-3 binding to the Samd-3 binding site at ?12 is essential for transcriptional activation of pST3Gal I in TGF-?1-induced PK-15 cells.mRNA levels of pST3Gal I are increased in PK-15 cells induced by TGF-?TGF-?1 treatment increases the level of glycan structure synthesized by pST3Gal I. Smad-3 site is crucial for transcriptional activation of pST3Gal I by TGF-?1..


PMID: 21945615

DOI: 10.1016/j.bbrc.2011.09.043

Other references

Pińa Batista, K.M.; Vega, I.F.; de Eulate-Beramendi, S.A.; Morales, J.; Kurbanov, A.; Asnel, D.; Meilan, A.; Astudillo, A., 2016: Prognostic significance of the markers IDH1 and YKL40 related to the subventricular zone. Glioblastoma multiforme (GBM), a highly aggressive brain cancer characterized by uncontrolled proliferation, resistance to cell death, angiogenesis, and vascular edema, remains one of the deadliest types of cancer. The subventricular zone (SVZ) ha...

Nicita, G.; Villari, D.; Caroassai Grisanti, S.; Marzocco, M.; L.M.rzi, V.; Martini, A., 2016: Minimally Invasive Transanal Repair of Rectourethral Fistulas. Rectourethral fistulas (RUFs) represent an uncommon complication of pelvic surgery, especially radical prostatectomy. To date there is no standardised treatment for managing RUFs. This represents a challenge for surgeons, mainly because of the pot...

Fernandez Del Pozo, M.B.; Fernandez, E.; Cuellar, S., 2002: Development of continuing education to pharmacists. Methods & Findings in Experimental & Clinical Pharmacology 24(Suppl A): 121

Burts, E., 1974: Aerial application of pesticides. By using a new spinning dispenser producing small uniform droplets, aerial applications of only 1 pt/acre of undiluted malathion controlled cherry fruit fly [Rhagoletis spp.] and similar applications of phosphamidon controlled aphids in apple orch...

Stamp, Ne, 1982: Searching behaviour of parasitoids for web-making caterpillars: a test of optimal searching theory Apanteles euphydryidis, Euphydryas phaeton. Journal of animal ecology 51(2): 387-395

Dolka, C.; Van Hecke, K.; Van Meervelt, L.; Tsoungas, P.G.; Van der Eycken, E.V.; Varvounis, G., 2009: Novel thermal and microwave-assisted facile route to naphthalen-2(1H)-ones via an oxidative alkoxylation-ring-opening protocol. Several novel 1,1-disubstituted-8-hydroxynaphthalen-2(1H)-ones have been efficiently synthesized via a two-step sequence from 2-hydroxy-1-naphthaldehyde oxime. The methodology involves oxidative ring closure and alkoxylation to 3a-alkoxynaphtho[1,...

Idowu, A.B.; Akinsete, A., 2001: The attraction of Zonocerus variegatus (Orthoptera: Pyrgomorphidae) to different types of lure. Zonocerus variegatus is a common grasshopper in parts of west and equtorial Africa. The distribution in Nigeria extends from the lowland rainforest zone to the savannah in the north. The influence of lure on the behaviour of grasshopper inside cag...

Osara, K., 1962: The cultivation of crisp-leaved cabbage lettuce. Variety trials during the years 1957-58 indicate that the highest yielding variety is Pennlake, which also produces the largest heads, followed by 46 (Penna State).

Goodhue, L.D.; Baumgartner, F.M., 1965: Applications of new bird control chemicals. A new approach for the control of nuisance and depredatory birds is offered by the use of chemically treated baits which produce flock-disturbing reactions. Physiological effects involving unusual actions and calls disturb and warn unaffected bird...

Schouteden, H., 1969: Note sur trois oiseaux du Katanga. Revue de Zoologie et de Botanique Africaines, 80: 308-310