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Transcriptional activation of pig Galβ1,3GalNAc α2,3-sialyltransferase (pST3Gal I) gene by TGF-β1 in porcine kidney PK-15 cells


, : Transcriptional activation of pig Galβ1,3GalNAc α2,3-sialyltransferase (pST3Gal I) gene by TGF-β1 in porcine kidney PK-15 cells. Biochemical and Biophysical Research Communications 414(1): 159-164

In this study we investigated for the first time the transcriptional regulation of pig Gal?1,3GalNAc ?2,3-sialyltransferase (pST3Gal I) in response to TGF-?1 in porcine kidney PK-15 cells. The pST3Gal I gene was found to span about 9 kb and to be composed of 8 exons including 2 exons in the 5?-untranslated region. RT-PCR analysis indicated that the induction of pST3Gal I by TGF-?1 is regulated at the transcriptional level. Functional analysis of the 5?-flanking region of the pST3Gal I gene revealed the ?1257 to ?976 region functions as the TGF-?1-inducible promoter and that the Smad-binding site at ?12 is crucial for TGF-?1-induced expression of pST3Gal I in PK-15 cells. In addition, the transcriptional activity of pST3Gal I induced by TGF-?1 in PK-15 cells was strongly inhibited by SIS3, which is a specific Smad-3 inhibitor. In summary, our results identified the core promoter region in the pST3Gal I promoter and demonstrated that Smad-3 binding to the Samd-3 binding site at ?12 is essential for transcriptional activation of pST3Gal I in TGF-?1-induced PK-15 cells.mRNA levels of pST3Gal I are increased in PK-15 cells induced by TGF-?TGF-?1 treatment increases the level of glycan structure synthesized by pST3Gal I. Smad-3 site is crucial for transcriptional activation of pST3Gal I by TGF-?1..

US$19.90

PMID: 21945615

DOI: 10.1016/j.bbrc.2011.09.043


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