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Human umbilical cord Wharton's jelly stem cells and its conditioned medium support hematopoietic stem cell expansion ex vivo

, : Human umbilical cord Wharton's jelly stem cells and its conditioned medium support hematopoietic stem cell expansion ex vivo. Journal of Cellular Biochemistry 113(2): 658-668

Bone marrow mesenchymal stromal cells (BMMSCs) have been used as feeder support for the ex vivo expansion of hematopoietic stem cells (HSCs) but have the limitations of painful harvest, morbidity, and risk of infection to the patient. This prompted us to explore the use of human umbilical cord Wharton's jelly MSCs (hWJSCs) and its conditioned medium (hWJSC-CM) for ex vivo expansion of HSCs in allogeneic and autologous settings because hWJSCs can be harvested in abundance painlessly, are proliferative, hypoimmunogenic, and secrete a variety of unique proteins. In the presence of hWJSCs and hWJSC-CM, HSCs put out pseudopodia-like outgrowths and became highly motile. Time lapse imaging showed that the outgrowths helped them to migrate towards and attach to the upper surfaces of hWJSCs and undergo proliferation. After 9 days of culture in the presence of hWJSCs and hWJSC-CM, MTT, and Trypan blue assays showed significant increases in HSC numbers, and FACS analysis generated significantly greater numbers of CD34(+) cells compared to controls. hWJSC-CM produced the highest number of colonies (CFU assay) and all six classifications of colony morphology typical of hematopoiesis were observed. Proteomic analysis of hWJSC-CM showed significantly greater levels of interleukins (IL-1a, IL-6, IL-7, and IL-8), SCF, HGF, and ICAM-1 compared to controls suggesting that they may be involved in the HSC multiplication. We propose that cord blood banks freeze autologous hWJSCs and umbilical cord blood (UCB) from the same umbilical cord at the same time for the patient for future ex vivo HSC expansion and cell-based therapies.


PMID: 21976004

DOI: 10.1002/jcb.23395

Other references

Husain, S., 2014: Reconstruction of moderately depressed nose in leprosy (a long-term follow-up). Fifty seven leprosy patients having moderately collapsed nose were taken for nasal reconstruction. The bone graft was obtained from the second metatarsal of the foot. It was corticoperiosteal and placed in between lining and the nasal skin. 48 pat...

Dehwah, A.H.A.; Missimer, T.M., 2017: Seabed gallery intakes: Investigation of the water pretreatment effectiveness of the active layer using a long-term column experiment. Seabed gallery intake systems used for seawater reverse osmosis facilities employ the same principle of water treatment as slow sand filtration in freshwater systems. An investigation concerning the effectiveness of the active layer (top layer) in...

Kooiker, M.J.G.; Pel, J.J.M.; van der Steen, J., 2016: The relationship between visual orienting responses and clinical characteristics in children attending special education for the visually impaired. We recently introduced a method based on quantification of orienting responses toward visual stimuli to assess the quality of visual information processing in children. In the present study, we examined the relationship between orienting responses...

Choudhury, S.; Gupta, P.; Ghosh, S.; Mukherjee, S.; Chakraborty, P.; Chatterji, U.; Chattopadhyay, S., 2016: Arsenic-induced dose-dependent modulation of the NF-κB/IL-6 axis in thymocytes triggers differential immune responses. Arsenic contamination of drinking water is a matter of global concern. Arsenic intake impairs immune responses and leads to a variety of pathological conditions including cancer. In order to understand the intricate tuning of immune responses elic...

Reif Lehrer L., 1980: Effects of glutamate on isolated chick embryo retina. Federation Proceedings: Abstract 2935

Mandreoli, M.; Zuccala, A.; Losinno, F.; Mambelli, E.; Zuccchelli, P., 1999: Does antiphospholipid antibodies play a role in renal artery stenosis?. Nephrology Dialysis Transplantation 14(9): A66, Sept

Knezevic, Pf, 1971: Movable operation table for large animal in limb surgery. Wiener tierarztliche Monatsschrift: 58 (4) 173-176

Holub D.A.; Luper W.E.; Fuhrman T.M.; Edmonds C.H.; Mcgee M.G.; Norman J.C., 1978: Coagulation profiles during abdominal left ventricular assist device support in man. Clinical Research 26(5): 648A

Shearer J.D.; Bereiter D.F.; Mastrofrancesco B.; Caldwell M.D., 1990: Lymphocyte substrate utilization in the local wound environment. FASEB Journal 4(3): A639

Ong, D.; Harper, C.Alex.; Fagan, X., 2017: Clinical case of influenza A with outer retinal maculopathy. Clinical & Experimental Ophthalmology 45(7): 745-747