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Species-specific inhibitor sensitivity of angiotensin-converting enzyme 2 (ACE2) and its implication for ACE2 activity assays


, : Species-specific inhibitor sensitivity of angiotensin-converting enzyme 2 (ACE2) and its implication for ACE2 activity assays. American Journal of Physiology. Regulatory, Integrative and Comparative Physiology 301(5): R1293-9

Angiotensin-converting enzyme 2 (ACE2) is a component of the renin-angiotensin system, and its expression and activity have been shown to be reduced in cardiovascular diseases. Enzymatic activity of ACE2 is commonly measured by hydrolysis of quenched fluorescent substrates in the absence or presence of an ACE2-specific inhibitor, such as the commercially available inhibitor DX600. Whereas recombinant human ACE2 is readily detected in mouse tissues using 1 μM DX600 at pH 7.5, the endogenous ACE2 activity in mouse tissues is barely detectable. We compared human, mouse, and rat ACE2 overexpressed in cell lines for their sensitivity to inhibition by DX600. ACE2 from all three species could be inhibited by DX600, but the half maximal inhibitory concentration (IC(50)) for human ACE2 was much lower (78-fold) than for rodent ACE2. Following optimization of pH, substrate concentration, and antagonist concentration, rat and mouse ACE2 expressed in a cell line could be accurately quantified with 10 μM DX600 (>95% inhibition) but not with 1 μM DX600 (<75% inhibition). Validation that the optimized method robustly quantifies ACE2 in mouse tissues (kidney, brain, heart, and plasma) was performed using wild-type and ACE2 knockout mice. This study provides a reliable method for measuring human, as well as endogenous ACE2 activity in rodents. Our data underscore the importance of validating the effect of DX600 on ACE2 from each particular species at the experimental conditions employed.

US$19.90

PMID: 21880865

DOI: 10.1152/ajpregu.00339.2011


Other references

Walshe, F.M.R., 1950: Humanism, history, and natural science in medicine. British Medical Journal 2(4675): 379-384

Nielsen, P.S.; Clark, A.J.; Oliver, R.P.; Huber, M.; Spanu, P.D., 2001: HCf-6, a novel class II hydrophobin from Cladosporium fulvum. C. fulvum [Fulvia fulva], a fungal tomato pathogen, has previously been shown to express a complex family of hydrophobin genes including four class I hydrophobins and one class II hydrophobin. Here we describe a gene for HCf-6, a sixth member of t...

Tewari, R.; Choudhury, S.Roy.; Mehta, V.Singh.; Sen, E., 2012: TNFα regulates the localization of CD40 in lipid rafts of glioma cells. Resistance of glioblastoma multiforme (GBM) to TNFα induced apoptosis is attributed to NFκB activation. As TNF-receptor family member CD40 regulates NFκB activation, we investigated the role of CD40 in NFκB activation in GBM. We observed eleva...

Maggrett, Harold I., 1940: The ability of certain common trees to withstand drought in southeastern South Dakota. The field survey was taken over a period of 5 yrs. from 1934 to 1939 and included 12 of the commonest spp. of trees[long dash]-red cedar (97%), Chinese elm (97%), hackberry (96%), Ponderosa pine (92%), honey locust (88%), bur oak (85%), American e...

Waclawovsky, G.; Lehnen, A.Machado., 2016: Hemodynamics of aerobic and resistance blood flow restriction exercise in young and older adults. Light-load blood flow restriction exercise (BFRE) may provide a novel training method to limit the effects of age-related muscle atrophy in older adults. Therefore, the purpose of this study was to compare the haemodynamic response to resistance a...

Cai Z.; Brauen S.E.; Gealy D.R.; Johnston W.J.; Lumpkin T.A., 1992: Tolerance of chinese milkvetch astragalus sinicus to herbicides. Chinese milkvetch is the most important green manure crop in the rice producing regions of China. The tolerance of Chinese milkvetch to 29 herbicides approved for other legume crops was determined in the greenhouse. Chinse milkvetch was tolerant t...

Kim, S.K.; Ang, L.; Hsu, Y.Y.; Dorscher-Kim, J.; Kim, S., 1996: Antagonistic effect of D-myo-inositol-1,2,6-trisphosphate (PP56) on neuropeptide Y-induced vasoconstriction in the feline dental pulp. Intra-arterial injection of neuropeptide Y (NPY) (1.3-2.0 micrograms/kg) resulted in decreases of pulpal blood flow by 37.7 +/- 5.7% (mean +/- SEM). The intra-arterial injection of D-myo-inositol-1,2,6-trisphosphate (PP56) (0.3 mg/kg) alone change...

Bavestrello, G.; Boero, F., 1988: Necrosi e rigenerazione in Eunicella cavolinii (Anthozoa, Cnidaria) in Mar Ligure. Bollettino dei Musei e degli Istituti Biologici dell'Universita di Genova1988]; 52(Suppl.): 295-300

Hoang, V-An.; Kim, Y-Ju.; Nguyen, N.Lan.; Kang, C.Ho.; Kang, J-Pyo.; Singh, P.; Farh, M.El-Agamy.; Yang, D-Uk.; Yang, D-Chun., 2016: Microbacterium rhizomatis sp. nov., a β-glucosidase-producing bacterium isolated from rhizome of Korean mountain ginseng. A novel Gram-staining-positive, rod-shaped bacterium, designated DCY100(T), was isolated from rhizome of mountain ginseng root in Hwacheon mountain, Gangwon province, Republic of Korea. The 16S rRNA gene sequence analysis showed that strain DCY100...

Savich R.D.; Cummings C.; Scott S.M.; Kelly G., 1992: The role of growth factors on fetal lung growth during oligohydramnios. Pediatric Research 31(4 PART 2): 322A