+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro

, : Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro. Journal of Ocean University of China 10(4): 369-378

Effects of carboxymethyl-chitosan with different molecular weights on wound healing were investigated in vivo and in vitro. A second degree burn model was performed in rats and the accelerative effects of carboxymethyl-chitosan on wound repair were observed. Contents of transforming growth factor (TGF)-?1, interleukin(IL)-6 and matrix metalloproteinase (MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay (ELISA). In vitro study evaluated the influence of carboxymethyl-chitosan on cytokines secretion of fibroblasts and macrophages. In vivo results showed that carboxymethyl-chitosan effectively accelerated the wound healing process in burned rats (P<.5). Levels of TGF-?1, IL-6 and MMP-1 in carboxymethyl-chitosan groups were significantly elevated, compared with control group (P<.5). In vitro results indicated that carboxymethyl-chitosan significantly promoted the proliferation of fibroblasts and stimulated its IL-8 and IL-1 secretion at different incubation time, but it did not affect collagen secretion of fibroblasts. Carboxymethyl-chitosan enhanced phagocytosis ability of macrophages, and increased its tumor necrosis factor (TNF)-? secretion. In conclusion, carboxymethyl-chitosan promoted wound healing by activating macrophages, accelerating fibroblasts growth and exerting considerable effects on the secretion of a series of cytokines.


DOI: 10.1007/s11802-011-1764-y

Other references

Chaki, O.; Gorai, I.; Mochizuki, K.; Yoshikata, H.; Kikuchi, R.; Hirahara, F., 2000: Hormone replacement therapy is more effective on bone mass in late postmenopausal Japanese women than in early ones. Journal of Bone & Mineral Research 15(Suppl 1): S555, September

Lal, P.; Guegler, K.J.; Corley, N.C., 2000: Prostate growth-associated membrane proteins. The invention provides two human prostate growth-associated membrane proteins (PGAMP) and polynucleotides which identify and encode PGAMP. The invention also provides expression vectors, host cells, antibodies, agonists, and antagonists. The inven...

Atalar, O.; Yilmaz, S.; Dinç, G.; Ozdemir, D., 2004: The venous drainage of the heart in porcupines (Hystrix cristata). The venous drainage of the heart in porcupines (Hystrix cristata) was investigated. Each of the five adult porcupines (three males, two females) was injected with a coloured latex mixture via the V. cava caudalis for the demonstration of the cardi...

Mishra, S.S.; Datta, K.C., 1962: A preliminary pharmacological study of Ipomoea digitata Linn. Fraction D (ether-insoluble, alcohol-soluble fraction) of Ipomoea digitata Linn, was investigated. It is a powerful stimulant of blood pressure and respiration. The pressor action has a peripheral component and a small central component. The drug...

Banowetz, G.M.; Hess, J.R.; Carman, J.G., 1994: A monoclonal antibody against the plant growth regulator, abscisic acid. Monoclonal antibodies were prepared against the plant growth regulator abscisic acid (ABA) conjugated to keyhole limpet hemocyanin through C-4. One of these antibodies was characterized for use in a competition fluorescence enzyme-linked immunosor...

Anonymous, 1968: Cadmium and metabolism of albumin. Chronic cadmium poisoning in industrial workers and animals in relation to urinary protein excretion is discussed.

Carré, B.; Lessire, M.; Juin, H., 2015: Prediction of the net energy value of broiler diets. Thirty pelleted diets were given to broiler chickens (eight birds per diet; 21 to 35 days of age) for individual in vivo measurements of dietary net energy (NE) value, using three trials with 10 diets/trial. Amino acid formulation of diets was don...

Kataoka, S.; Hori, A.; Shirakawa, T.; Hirose, G., 1997: Paramedian pontine infarction. Neurological/topographical correlation. Background and Purpose: There have been few reports of pontine syndromes secondary to paramedian pontine infarctions. To clarify the clinicotopographical correlation and prognosis of paramedian pontine infarct syndromes, we analyzed the clinical s...

Speroni, G., 1956: I fitofarmaci. Chim Indust : 944-964

Minami, K.; el-Banayosy, A.; Posival, H.; Seggewiss, H.; Murray, E.; Körner, M.M.; Körfer, R., 1992: Improvement of survival rate in patients with cardiogenic shock by using nonpulsatile and pulsatile ventricular assist device. Between January 1988 and January 1992, 65 patients (pts) had a ventricle assist device (VAD) inserted in our clinic. In 24 pts a VAD was applied because of primarily unsuccessful weaning from cardiopulmonary bypass (Group A). In a further 24 pts (...