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Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro


, : Effects of carboxymethyl-chitosan on wound healing in vivo and in vitro. Journal of Ocean University of China 10(4): 369-378

Effects of carboxymethyl-chitosan with different molecular weights on wound healing were investigated in vivo and in vitro. A second degree burn model was performed in rats and the accelerative effects of carboxymethyl-chitosan on wound repair were observed. Contents of transforming growth factor (TGF)-?1, interleukin(IL)-6 and matrix metalloproteinase (MMP)-1 in wounds were determined by enzyme-linked immunosorbent assay (ELISA). In vitro study evaluated the influence of carboxymethyl-chitosan on cytokines secretion of fibroblasts and macrophages. In vivo results showed that carboxymethyl-chitosan effectively accelerated the wound healing process in burned rats (P<.5). Levels of TGF-?1, IL-6 and MMP-1 in carboxymethyl-chitosan groups were significantly elevated, compared with control group (P<.5). In vitro results indicated that carboxymethyl-chitosan significantly promoted the proliferation of fibroblasts and stimulated its IL-8 and IL-1 secretion at different incubation time, but it did not affect collagen secretion of fibroblasts. Carboxymethyl-chitosan enhanced phagocytosis ability of macrophages, and increased its tumor necrosis factor (TNF)-? secretion. In conclusion, carboxymethyl-chitosan promoted wound healing by activating macrophages, accelerating fibroblasts growth and exerting considerable effects on the secretion of a series of cytokines.

US$19.90

DOI: 10.1007/s11802-011-1764-y


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