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The use of glass beads cultivation system to study the global effect of the ppk gene inactivation in Streptomyces lividans

, : The use of glass beads cultivation system to study the global effect of the ppk gene inactivation in Streptomyces lividans. Folia Microbiologica 56(6): 519-525

The glass beads cultivation system developed in our laboratory for physiological studies of filamentous microorganisms supports differentiation and allows complete recovery of bacterial colonies and their natural products from cultivation plates. Here, we used this system to study the global effect of ppk gene disruption in Streptomyces lividans. The ppk encoding the enzyme polyphosphate kinase (P) catalyses the reversible polymerisation of gamma phosphate of ATP to polyphosphates. The resulting are phosphate and energy stock polymers. Because P activity impacts the overall energetic state of the cell, it is also connected to secondary metabolite (e.g. antibiotic) biosynthesis. We analysed the global effects of the disruption of this gene including its influence on the production of pigmented antibiotics, on morphological differentiation, on the levels of ATP and on the whole cytoplasmic protein expression pattern of S. lividans. We observed that the S. lividans ppk mutant produced antibiotics earlier and in greater amount than the wild-type (wt) strain. On the other hand, we did not observe any obvious effect on colony morphological development. In agreement with the function of Ppk, we detected much lower levels of ATP in ppk- mutant than in the wt strain. Proteomic analysis revealed that the genes that were influenced by ppk inactivation included enzymes involved in carbon or nitrogen metabolism, phosphate transport and components of the cell translational machinery. We showed that the synthesis of translation elongation factor Tu is during sporulation much higher in ppk- mutant than in wild-type strain.


PMID: 22083784

DOI: 10.1007/s12223-011-0076-3

Other references

Whitehouse, M.W., 1977: Some biochemical complexities of inflammatory disease affecting drug action. Agents and Actions. Supplements 2: 135-147

Rubini, A.; Gionfriddo, M.;, L.; Thrall, R., 1996: Mechanical properties of the respiratory system and work of breathing in oleic acid induced lung injury in the rat. Six rats were anesthetized, trachea was cannulated, an i.v. line placed in the femoral vein; the rats were mechanically ventilated and placed in a body-box. Tidal volume was measured from the body-box pressure, and airway pressure was measured pro...

Ten Bruggencate, S.J.M.; Bovee-Oudenhoven, I.M.J.; Lettink-Wissink, M.L.G.; Katan, M.B.; van der Meer, R., 2005: Dietary fructooligosaccharides affect intestinal barrier function in healthy men. In contrast to most expectations, we showed previously that dietary fructooligosaccharides (FOS) stimulate intestinal colonization and translocation of invasive Salmonella enteritidis in rats. Even before infection, FOS increased the cytotoxicity...

Kidd Robert B.; Jipa Dan C.; Weser Oscar E., 1975: Sedimentary processes in the development of the Indus submarine fan. International Congress on Sedimentology = Congres International de Sedimentologie: eme 6; Pages 81-86. 1975.

Sanjaume E.; Segura F.; Lopez Garcia M.J.; Pardo J., 1992: Recent sedimentation in Valencia Lagoon; preliminary results. Journal of Coastal Research: Pages 688-698. 1992.

Bohlius, J.F.; Langensiepen, S.; Schwarzer, G.; Engert, A., 2002: Epoietin in the Treatment of Malignant Disease A Comprehensive Meta-Analysis. Background: Cancer and cancer therapy associated anaemia represents an important economic factor. Therefore, therapeutic alternatives such as erythropoietin (epo) and red blood cell transfusions have to be evaluated systematically. We conducted a...

Takasu, K.; Kasuya, N.; Honda, M.; Tanabe, M.; Ono, H., 2004: Role of the descending noradrenergic system and spinal alpha2-adrenoceptors in ameliorating effects of gabapentin on hyperalgesia and tactile allodynia. Journal of Pharmacological Sciences 94(Supplement 1): 170P

Catalano, K., 1994: AMI--Joint Commission multihospital survey process. Health Systems Review 27(4): 26, 28-26, 28

Trifonova E.; Chemberski Kh; Vantsurova A., 1989: Correlation of part of the triassic system in borehole sections from northwest bulgaria. Dragov, P (Ed ) Monograph Of The Bulgarian Geological Society, No 9 The Geology And Mineral Resources Of Northwestern Bulgaria 286p The State Publishing House "tekhnika": Sofia, Bulgaria Illus Maps : 11-16

Anhert, W., 1970: Assistant thinks about her work. Monatsschrift Deutscher Zahnarzte: der Freie Zahnarzt 14(11): 17-18