geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of a protein kinase A regulatory subunit from Leishmania having importance in metacyclogenesis through induction of autophagy


, : Identification of a protein kinase A regulatory subunit from Leishmania having importance in metacyclogenesis through induction of autophagy. Molecular Microbiology 83(3): 548-564

cAMP-mediated responses act as modulators of environmental sensing and cellular differentiation of many kinetoplastidae parasites including Leishmania. Although cAMP synthesizing (adenylate cyclase) and degrading (phosphodiesterase) enzymes have been cloned and characterized from Leishmania, no cAMP-binding effector molecule has yet been identified from this parasite. In this study, a regulatory subunit of cAMP-dependent protein kinase (Ldpkar1), homologous to mammalian class I cAMP-dependent protein kinase regulatory subunit, has been identified from L. donovani. Further characterization suggested possible interaction of LdPKAR1 with PKA catalytic subunits and inhibition of PKA activity. This PKA regulatory subunit is expressed in all life cycle stages and its expression attained maximum level in stationary phase promastigotes, which are biochemically similar to the infective metacyclic promastigotes. Starvation condition, the trigger for metacyclogenesis in the parasite, elevates LdPKAR1 expression and under starvation condition promastigotes overexpressing Ldpkar1 attained metacyclic features earlier than normal cells. Furthermore, Ldpkar1 overexpression accelerates autophagy, a starvation-induced cytological event necessary for metacyclogenesis and amastigote formation. Conditional silencing of Ldpkar1 delays the induction of autophagy in the parasite. The study, for the first time, reports the identification of a functional cAMP-binding effector molecule from Leishmania that may modulate important cytological events affecting metacyclogenesis.

US$19.90

PMID: 22168343

DOI: 10.1111/j.1365-2958.2011.07950.x


Other references

Schlee S., 1980: The research vessel atlantis and her 1st oceanographic expedition. Sears, M And D Merriman (Ed ) Oceanography: The Past; 3rd International Congress on The History Of Oceanography, Woods Hole, Mass , Usa, Sept 22-26, Xx+812p Springer-Verlag: New York, N Y , Usa; Berlin, West Germany Illus Maps P49-56

Listvin, K.S., 1954: The effectiveness of granulated superphosphate applied to flax and its residual effect on spring wheat.. When super was applied in the autumn under the plough or in spring under the cultivator, granular super was not greatly superior to powdered super in increasing the yields of flax straw and seed and the yield of the following wheat crop. Granular...

Copelli, S.; Targovnik, H.; Bergada, C., 1995: Molecular sex determination. Significance in the diagnosis of gonadal pathologies. Sex differentiation follows two steps: sex determination where gender follows from the development of the embryonic gonads such as testes or ovaries and sex differentiation leading to the formation of internal and external genitalia. In man, testi...

Stone, T.W., 1980: Adenosine and related compounds do not affect nerve terminal excitability in rat CNS. Brain Research 182(1): 198-200

Karner G.D.; Steckler Michael S., 1983: Thermal and mechanical properties of the continental lithosphere and implications for the development of sedimentary basins. Eos, Transactions, American Geophysical Union 64(18): 322

Gu, J.D.; Li, J.; Wang, Y., 2005: Biochemical pathway and degradation of phthalate ester isomers by bacteria. Degradation of dimethyl isophthalate (DMI) and dimethyl phthalate ester (DMPE) was investigated using microorganisms isolated from mangrove sediment of Hong Kong Mai Po Nature Reserve. One enrichment culture was capable of utilizing DMI as the sol...

Kopystyanskiy R.S. ), 1983: Problems of oil-gas reservoir rocks at great depths. Geologiya i Geokhimiya Goryuchikh Iskopayemykh: ages 63-67. 1983.

Scott, R.R., 1937: Annual medical report for 1935. This official report is devoted to an account of the health and sanitary conditions of the Tanganyika Territory during 1935. The following are some of the more frequently encountered diseases: syphilis, malaria, yaws, ankylostomiasis, taeniasis, a...

Harriman, S.; Rodych, N.; Hayes, P.; Moser, M.A.J., 2011: The C-reactive protein-to-prealbumin ratio predicts fistula closure. Of this study was to evaluate the predictability of fistula closure using the ratio of C-reactive protein to prealbumin (C:P ratio). : A database of 89 patients with gastrointestinal fistulas (1994 29) was created based on the records of our Nutri...

Chumachenko, N.V.; Sokolova, E.M., 1964: Effect of Bicillin on the bactericidal factors of the aseptic exudate of white rats. Bicillin administered into the inflammation focus in therapeutic doses was studied for its effect on the plasmocytic reaction lymphatic nodules and the bactericidal factors of aseptic purulent exudate of rats. Bicillin decreased the number of plas...