geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization and hepatocytes adhesion of galactosylated polyD,L-lactic-co-glycolic acid surface


, : Characterization and hepatocytes adhesion of galactosylated polyD,L-lactic-co-glycolic acid surface. Macromolecular Research 20(1): 93-100

The present study demonstrated that covalently galactosylated poly(D,L-lactic-co-glycolic acid) (PLGA) surface encourages hepatocyte adhesion and growth to form a dense cell network. Galactosylation of the PLGA surface was accomplished by grafting allylamine (AA) using inductively coupled plasma-assisted chemical vapor deposition (ICP-CVD) and conjugating lactobionic acid (LA) with AA via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) activation. The modified surface was characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance, electron spectroscopy of chemical analysis, atomic force microscopy, and contact angle measurement. For evaluation of cell affinity in vitro, primary rat hepatocytes were prepared and seeded onto the modified PLGA surfaces. The galactosylated PLGA surface showed more pronounced hepatocyte adhesion and growth compared to those on the control PLGA surface. The hepatocytes seeded on galactosylated substrates exhibited a radial migration with filopodial growth to form multicellular aggregates, whereas those on control PLGA showed slowly adhered rounded shapes. Moreover, galactosylation increased metabolic hepatocyte activities such as albumin secretion and urea synthesis.

US$19.90

DOI: 10.1007/s13233-012-0005-6


Other references

Lantz, A.L., 1913: U. Cyren, O. Uber die Identitat von Rana macrocnemis und R. Camerani Zool. Anz Leipzig, 43 214-220

Reis, B.Sgarbi.; Jungbluth, A.A.; Frosina, D.; Holz, M.; Ritter, E.; Nakayama, E.; Ishida, T.; Obata, Y.; Carver, B.; Scher, H.; Scardino, P.T.; Slovin, S.; Subudhi, S.K.; Reuter, V.E.; Savage, C.; Allison, J.P.; Melamed, J.; Jäger, E.; Ritter, G.; Old, L.J.; Gnjatic, S., 2014: Prostate cancer progression correlates with increased humoral immune response to a human endogenous retrovirus GAG protein. Clinical Cancer Research 19(22): 6112-6125

Koechlin, P.; Nael, J.F.; Bonnet, J.C.; D'Ythurbide, B.; Apoil, A., 1983: Ligamentous lesions associated with fractures of the tibial plateau. Acta Orthopaedica Belgica 49(6): 751-760

Julin, Ch., 1893: Le corps vitellin de Balbiani et les elements de la cellule des Metazoaires qui correspondent au macronucleua des Infusoires cilies. Bulletin Scientifique de la France et de la Belgique, xxv1: 295-345

Brant, K.A.; Fabisiak, J.P., 2009: Nickel and the microbial toxin, MALP-2, stimulate proangiogenic mediators from human lung fibroblasts via a HIF-1alpha and COX-2-mediated pathway. Hypoxia-inducible factor (HIF-1alpha) and cyclooxygenase-2 (COX-2) have been implicated in the regulation of inflammatory-like processes that lead to angiogenesis and fibrotic disorders. Here we demonstrate that in human lung fibroblasts (HLFs) tr...

Cabrini, T.M.deiros Barbosa; Cardoso, R.S.lva, 2012: Population Biology of Nassarius vibex Say, 1822 on a Sheltered Beach in Southeastern Brazil. Nassariids have a worldwide distribution and are most often found in sheltered embayments in tropical, subtropical, and temperate zones occurring in intertidal and subtidal areas. Species of this genus are scavengers, constituting a major link in...

Harris, W.E., 1971: Pseudoendodontic sinus tract: report of case. Journal of the American Dental Association 83(1): 165-167

Schwegmann J.P.; Enzenauer R.J., 1992: Psoriatic arthritis case report. Contemporary Orthopaedics 25(1): 62-64

Klauser, A.S.; D.Z.rdo, T.; Bellmann-Weiler, R.; Feuchtner, G.M.; Sailer-Höck, M.; Sögner, P.; Gruber, J., 2009: Feasibility of second-generation ultrasound contrast media in the detection of active sacroiliitis. To determine whether a recently available contrast-enhanced ultrasound (CEUS) technique using second-generation microbubbles allows for the detection of active sacroiliitis, and to measure CEUS enhancement depth at the dorsocaudal part of the sacr...

Yang, B.; Balakrishnan, N.; Zhang, P.; Wang, X.; Bowman, J.M.; Forrey, R.C.; Stancil, P.C., 2016: Full-dimensional quantum dynamics of CO in collision with H2. Inelastic scattering computations are presented for collisions of vibrationally and rotationally excited CO with H2 in full dimension. The computations utilize a newly developed six-dimensional potential energy surface (PES) and the previously rep...