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Swietenia macrophylla extract promotes the ability of Caenorhabditis elegans to survive Pseudomonas aeruginosa infection


, : Swietenia macrophylla extract promotes the ability of Caenorhabditis elegans to survive Pseudomonas aeruginosa infection. Journal of Ethnopharmacology 139(2): 657-663

Swietenia macrophylla or commonly known as big leaf mahogany, has been traditionally used as an antibacterial and antifungal agentof the studyThe unwanted problem of antibiotic resistance in many bacterial species advocates the need for the discovery of the new anti-infective drugs. Here, we investigated the anti-infective properties of S. macrophylla with an assay involving lethal infection of Caenorhabditis elegans with the opportunistic human pathogen Pseudomonas aeruginosaMaterials Using a slow killing assay, C. elegans was challenged with an infective strain of P. aeruginosa (PA14). The ability of S. macrophylla seed ethyl acetate extract to promote the survival of infected worms was assessed by comparing the percentage of survival between extract treated and non-treated worm populations. The effect of S. macrophylla towards PA14 growth, C. elegans feeding rate and degree of PA14 colonization in the worm gut was also evaluated. Lastly, using a fluorescent transgenic C. elegans strain and real time PCR, the effect of S. macrophylla on the expression of lys-7, an immune response gene was also investigatedResultsOur results demonstrate the ability of S. macrophylla seed ethyl acetate extract in rescuing C. elegans from fatal PA14 infection. Consequently, we showed that the extract promotes the survival without exhibiting any bactericidal effect or perturbation of C. elegans feeding rate. We also showed that S. macrophylla was able to restore the initially repressed lys-7 level in PA14 infected C. elegansConclusionS. macrophylla extract is able to enhance the ability of C. elegans to survive PA14 infection without directly killing the pathogen. We further showed that the extract boosted the expression of a gene pivotal for innate immunity in C. elegans. Collectively, these findings strongly suggest the presence of compounds within S. macrophylla seed that either reduces P. aeruginosa virulence and/or enhance host resistance.

US$19.90

PMID: 22193176

DOI: 10.1016/j.jep.2011.12.016


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