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Identification of hydrophobic tags for the degradation of stabilized proteins

, : Identification of hydrophobic tags for the degradation of stabilized proteins. Chembiochem 13(4): 538-541

New HyTs are a knockout: We previously reported that labeling HaloTag proteins with low molecular weight hydrophobic tags (HyTs) leads to targeted degradation of HaloTag fusion proteins. In this report, we employed a chemical approach to extend this hydrophobic tagging methodology to highly stabilized proteins by synthesizing and evaluating a library of HyTs, which led to the identification of HyT36.


PMID: 22271667

DOI: 10.1002/cbic.201100793

Other references

Abramson, N.; Bjelkhagen, H., 1978: Pulsed sandwich holography. 2: Practical application. The vibration of a small electric drilling machine was studied holographically. The machine was held by hand, and drilling was done on a steel plate during two exposures from a double pulsed ruby laser. The recording was made on a rotating sandwic...

Li, Z.; Yang, S.; Inoue, Y.; Yoshida, N.; Katayama, A., 2011: Complete anaerobic mineralization of pentachlorophenol (PCP) under continuous flow conditions by sequential combination of PCP-dechlorinating and phenol-degrading consortia. Complete mineralization of 50 µM of pentachlorophenol (PCP) was achieved anaerobically under continuous flow conditions using two columns connected in series with a hydraulic retention time of 14.2 days, showing the highest reported mineralizat...

Jordan, A.M., 1961: An assessment of the economic importance of the tsetse species of Southern Nigeria and the Southern Cameroons based on their trypanosome infection rates and ecology. Previous records and new data on infection rates in species of Glossina inhabiting southern Nigeria and the Southern Cameroons are presented, and the probable economic importance of each species is assessed. The trypanosome infection rates were hi...

Brabander, D.-De; Aerts, J.; Boucque, C.; Buysse, F., 1982: Effect of the chopping length of maize silage on the voluntary intake and performances by dairy cattle. Revue de l'agriculture 35(6): 3181-3193

Kulbertus, H., 1976: Chronic atrio-ventricular blocks: etiology and epidemiology. Introduction. Acta Cardiologica Suppl 21: 5-8

Carr, K.D.; Hiller, J.M.; Simon, E.J., 1985: Diphenhydramine potentiates narcotic but not endogenous opioid analgesia. The analgesic effect of morphine in rats, as reflected in elevated thresholds for tail shock induced vocalizations, was markedly potentiated by the antihistamine, diphenhydramine. Intrinsic to the behavioral test paradigm employed are stressors wh...

Goble, R., 2002: New for 02--a look at the latest in farm equipment. California grower: avocados citrus subtropicals 26(2): 11-12

Luo, L.M.; Hamitouche, C.; Dillenseger, J.L.; Coatrieux, J.L., 1993: A moment-based three-dimensional edge operator. This paper presents a three-dimensional edge operator aimed at the detection of anatomical structures in medical imaging. It uses the spatial moments of gray level surface, and operates in three dimensions with any window size. It allows us to est...

Potier, A.; Dony, A.; Crapsky, A.; Ferry, O.; Piney, D.; Dufay, E., 2016: A Woman Experienced Severe Thrombocytopenia When Treated With Fluvastatin. A 62-year-old woman treated with fluvastatin experienced three separate thrombocytopenic illnesses, severe on two occasions associated with nadir platelet count of 57 000/µL and 75 000/µL. The hospital pharmacist replaced fluvastatin by pravas...

Rosenberg, P.E., 1959: Audiometric considerations in stapes-mobilization surgery. Stapes-mobiliza-tion surgery presents a less distinct prognosis for recovery of loss of hearing than fenestration. A case is presented calling attention to the fact that successful surgery on an ear with mixed deafness resulted in a marked loss of...