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Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes

, : Interleukin 18 messenger RNA and proIL-18 protein expression in chorioamniotic membranes from pregnant women with preterm prelabor rupture of membranes. European Journal of Obstetrics, Gynecology, and Reproductive Biology 161(2): 134-139

To quantify the expression of IL-18 mRNA and protein in the chorioamniotic membranes of pregnant women with PPROM and correlate expression with histological chorioamnionitis.Study designA case control study that included 42 pregnant women not in labor in the following groups: PPROM (n = 28) and controls with intact membranes submitted to selective cesarean section at term (n = 14). Expression of IL-18 mRNA in chorioamniotic membranes was determined by real-time polymerase chain reaction, and IL-18 protein expression was measured by western blot. Histopathological analyses and immunolocalization of IL-18 by immunohistochemistry were also performed. Analyses were performed using the Mann Whitney or Fisher's exact tests and the group effect was considered significant if the adjustedp-values were <.5 and the magnitude of change was greater than 2-fold for mRNA expression. IL-18 mRNA was present in 1% of samples and no difference in expression was observed between termvs.PPROM membranes (fold-change .12;p = .88). In the PPROM group, no difference was observed in IL-18 mRNA regarding gestational age (fold-change .11;p = .42) or the presence of histological chorioamnionitis (fold-change .26;p = .15). ProIL-18 was present in all samples. IL-18 was immunolocalized to amnion, chorion and decidua cells, with intense immunohistochemical staining at the choriodecidual junction. Chorioamniotic membranes are sources of IL-18 mRNA and proIL-18, and their expression is unrelated to PPROM or histological chorioamnionitis.


PMID: 22285684

DOI: 10.1016/j.ejogrb.2011.12.026

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