geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Tetracyclines convert the osteoclastic-differentiation pathway of progenitor cells to produce dendritic cell-like cells


, : Tetracyclines convert the osteoclastic-differentiation pathway of progenitor cells to produce dendritic cell-like cells. Journal of Immunology 188(4): 1772-1781

Tetracyclines, such as doxycycline and minocycline, are used to suppress the growth of bacteria in patients with inflammatory diseases. Tetracyclines have been shown to prevent bone loss, but the mechanism involved is unknown. Osteoclasts and dendritic cells (DCs) are derived from common progenitors, such as bone marrow-derived macrophages (BMMs). In this article, we show that tetracyclines convert the differentiation pathway, resulting in DC-like cells not osteoclasts. Doxycycline and minocycline inhibited the receptor activator of NF-B ligand (RANKL)-induced osteoclastogenesis of BMMs, but they had no effects on cell growth and phagocytic activity. They influenced neither the proliferation nor the differentiation of bone-forming osteoblasts. Surprisingly, doxycycline and minocycline induced the expression of DC markers, CD11c and CD86, in BMMs in the presence of RANKL. STAT5 is involved in DC differentiation induced by GM-CSF. Midostaurin, a STAT5-signaling inhibitor, and an anti–GM-CSF–neutralizing Ab suppressed the differentiation induced by GM-CSF but not by tetracyclines. In vivo, the injection of tetracyclines into RANKL-injected mice and RANKL-transgenic mice suppressed RANKL-induced osteoclastogenesis and promoted the concomitant appearance of CD11c+ cells. These results suggested that tetracyclines prevent bone loss induced by local inflammation, including rheumatoid arthritis and periodontitis, through osteoclast–DC-like cell conversion.

US$19.90

PMID: 22250082

DOI: 10.4049/jimmunol.1101174


Other references

Moore Clyde H., 1985: Diagenetic evolution of Upper Jurassic carbonates, interior salt basins, northern Gulf of Mexico. Program with Abstracts - Geological Association of Canada(Mineralogical Association of Canada(Canadian Geophysical Union, Joint Annual Meeting 10: A40

Mullen, P.F.ench; Lucey, J., 1992: Crayfish in Co Donegal. Irish Naturalists' Journal 24(3): 133

Vogel, G.; Trost, W.; Halbach, G., 1974: Protective action of silymarine in phalloidine intoxication in the mouse. Agressologie: Revue Internationale de Physio-Biologie et de Pharmacologie Appliquees Aux Effets de L'agression 15(4): 263-270

Anonymous, 1971: Physical properties of the Earth's mantle and core. Including a discussion of the origin and causes of seismic discontinuities.

Amemori, H., 1966: An experimental study of changes in the form of the mandible after extraction of lower posterior teeth. II. The changes in the form of the pars alveolaris mandibularis and the basis mandibulae. The present study was carried out to reveal the changes in the form of the pars alveolaris mandibularis and the basis mandibulae after extraction of teeth. The materials used in this study were 14 mongrel dogs which were divided into 8 groups and...

Camacho E.A., 1942: El origen de los temblores. States briefly facts to show that earthquakes are due to physical-chemical reactions between sea and land.

Bhatnagar, V.; Agarwala, S.; Lal, R.; Mitra, D.K., 2000: Fulguration of posterior urethral valves using the Nd:YAG laser. Endoscopic management is the accepted form of treatment for posterior urethral valves (PUV). The Nd:YAG laser has been in clinical use for many decades, but has been used only sporadically for ablating PUV. In this study, PUV were diagnosed by mic...

Oka H I., 1988: A survey of environmental stresses on crop fields as shown by changes in the structure of weed communities. This work aims at learning about the impact of environmental changes on crop fields in Taiwan as reflected by changes in the structure of weed communities. Weed communities were investigated on the assumption that the plants sprouting from buried...

Bender, J.M.; See, M.T.; Hanson, D.J.; Lawrence, T.E.; Cassady, J.P., 2006: Correlated responses in growth, carcass, and meat quality traits to divergent selection for testosterone production in pigs. The objective of this project was to characterize changes in growth, carcass yield, and meat quality traits in castrates and gilts in response to divergent selection for testosterone production. In generation 21, endogenous testosterone concentrat...

Aubert, M.; Blaho, J.A., 1999: The herpes simplex virus type 1 regulatory protein ICP27 is required for the prevention of apoptosis in infected human cells. The herpes simplex virus type 1 (HSV-1) ICP27 protein is an immediate-early or alpha protein which is essential for the optimal expression of late genes as well as the synthesis of viral DNA in cultures of Vero cells. Our specific goal was to char...