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HBV Directly Promote Collagen ? Expression of LX-2 Cells without Infection in vitro


, : HBV Directly Promote Collagen ? Expression of LX-2 Cells without Infection in vitro.

Collagen type ? is the major extracellular matrix component in liver fibrosis induced by various factors, including HBV. However, whether HBV particles directly influence collagen type ? synthesis in human hepatic stellate cells is poorly understood. Purified HBV particles were purified by sucrose density gradient centrifugation, serum HBV were collected from chronic HBV infection patients, then both HBV samples were tested for their ability to influence collagen ? expression in LX-2, a human hepatic stellate cell line. Furthermore, we detected evidence of HBV infection to LX-2, and used six different inhibitors or blockers to identify pathways regulating collagen ? expression. The 3 15 IU/ml purified/serum HBV increased collagen type ? mRNA expression to 2.2/3.2 and 1.3/1.5-folds at 24 and 48 h respectively. Collagen type ? protein in the supernatant of purified/serum HBV group also increased compared to control group (48. 8./384.4 6.8 vs 262.7 15.7 ng/ml, P<.5). However, the 3 17 IU/ml purified/serum HBV increased collagen type ? expression similar to that of 3 15 IU/ml, while 3 13 IU/ml group showed no effect. No evidence of HBV infection to LX-2 cells were found, HBIG alleviate HBV induced collagen ? expression. Neutralization of TGF-?, TNF-?, PDGF, ERK and TGF-? receptor had no obvious inhibitory effects, only inhibition of p38MAPK decreased collagen type ? mRNA expression by .5/.4 and .4/.3-fold at 24 and 48 h, respectively. It reduced collagen type ? protein in purified/serum HBV gourp for 48 h (252.1 14.1/251.7 18.8 vs 43.9 4.9/385. 4.2 ng/ml, P<.5). HBV directly promote collagen type ? expression of LX-2 cells without infection in vitro.

US$19.90

DOI: 10.1111/j.1872-034X.2012.01000.x


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