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Mapping mouse hemangioblast maturation from headfold stages


, : Mapping mouse hemangioblast maturation from headfold stages. Developmental Biology 365(1): 1-13

The mouse posterior primitive streak at neural plate/headfold stages (NP/HF, ~7.5dpc 8dpc) represents an optimal window from which hemangioblasts can be isolated. We performed immunohistochemistry on this domain using established monoclonal antibodies for proteins that affect blood and endothelial fates. We demonstrate that HoxB4 and GATA1 are the first set of markers that segregate independently to endothelial or blood populations during NP/HF stages of mouse embryonic development. In a subset of cells, both proteins are co-expressed and immunoreactivities appear mutually excluded within nuclear spaces. We searched for this particular state at later sites of hematopoietic stem cell emergence, viz., the aorta gonad mesonephros (AGM) and the fetal liver at 1.5 11.5dpc, and found that only a rare number of cells displayed this character. Based on this spatial temporal argument, we propose that the earliest blood progenitors emerge either directly from the epiblast or through segregation within the allantoic core domain (ACD) through reduction of cell adhesion and pSmad1/5 nuclear signaling, followed by a stochastic decision toward a blood or endothelial fate that involves GATA1 and HoxB4, respectively. A third form in which binding distributions are balanced may represent a common condition shared by hemangioblasts and HSCs. We developed a heuristic model of hemangioblast maturation, in part, to be explicit about our assumptions.

US$19.90

PMID: 22426104

DOI: 10.1016/j.ydbio.2012.02.023


Other references

Shibley L.D.; Krahenbuhl J.L., 1986: Mycobacterium leprae burdened macrophages from lepromatous granulomas are refractory to activation by lymphokines. Federation Proceedings 45(4): 1108

Gagne, W.; Gressitt, J., 1982: Conservation in New Guinea. Monographiae biologicae2(2): 945-966

Huet; Mariné, 1997: Metamemory Assessment and Memory Behavior in a Simulated Memory Professional Task. This study investigated metamemory knowledge related to a professional task and the relationships between metamemory knowledge and memory performance in a simulated professional task, which was a beverage-service job with memory constraints change...

Piwernetz, K.; Benedetti, M.M.; Johansen, K.S., 1993: Advanced health care initiatives in Europe on quality development, epidemiology and medical documentation. Since the St. Vincent Declaration was published in 1989, several different but logically linked activities have been initiated. In addition, there are projects in adjacent fields supporting each other by exchange of information and partial co-oper...

Ballou, D.R.; Mccray, M.D.; Salyer, J.S., 1995: Rapid determination of vancomycin susceptibility using MicroScan Rapid Pos MIC Type 2 panels. Abstracts of the General Meeting of the American Society for Microbiology 95(0): 46

Badenas, C.; Rodríguez-Revenga, L.; Morales, C.; Mediano, C.; Plaja, A.; Pérez-Iribarne, M.Mar.; Soler, A.; Clusellas, Núria.; Borrell, A.; Sánchez, M.Ángeles.; Miró, E.; Sánchez, A.; Milà, M.; Jiménez, W., 2011: Assessment of QF-PCR as the first approach in prenatal diagnosis. Quantitative fluorescent PCR (QF-PCR) has been used by many laboratories for prenatal diagnosis of the most common aneuploidies. QF-PCR is rapid, cost-effective, and suitable for automation and can detect most abnormalities diagnosed by convention...

Derpgol ts V.F., 1963: Printsipy ukrupnennoy estestvennoy klassifikatsii prirodnykh vod zemli. A new theory of the genesis and evolution of natural waters is proposed. It is suggested that 'endothegenic' water replace 'juvenile' water in the terminology now in use. 'Biogenic' waters would include all other wate...

Gilmour, E.; Campbell, S.M.; Loane, M.A.; Esmail, A.; Griffiths, C.E.M.; Roland, M.O.; Parry, E.J.; Corbett, R.O.; Eedy, D.; Gore, H.E.; Mathews, C.; Steel, K.; Wootton, R., 1998: Comparison of teleconsultations and face-to-face consultations: Preliminary results of a United Kingdom multicentre teledermatology study. The objective of this multicentre study was to undertake a systematic comparison of face-to-face consultations and teleconsultations performed using low-cost videoconferencing equipment. One hundred and twenty-six patients were enrolled by their g...

Chen, L.; Yang, S.; Li, J.; Li, J.; Liu, Y.; Wang, Z.; Yang, B.; Li, T.; Li, H.; Bao, Z.; Zhuang, D.; Liu, S.; Li, L., 2011: Near full-length genomic characterization of a novel HIV type 1 CRF07_ BC/CRF08_ BC recombinant strain from Yunnan, China. Yunnan province was considered the HIV-1 epicenter of China, where many subtypes and CRFs of HIV-1 were circulating. CRF07_BC and CRF08_BC were two of the main circulating subtypes that caused more than 90% of the HIV-1 infections in intravenous d...

Oleinikova E.A.; Antipova V.N., 1985: Specific features of the immune responsiveness of the body in streptococcal carriage. The use of the discrete dynamic method for the treatment of data obtained in the survey of streptococcus carriers has made it possible to find out that their immune status is determined not so much by the quantitative changes in the results of ind...