+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Attachment of Escherichia coli O157:H7 to abiotic surfaces of cooking utensils

, : Attachment of Escherichia coli O157:H7 to abiotic surfaces of cooking utensils. Journal of Food Science 77(4): M194-M199

We examined the attachment of enterohemorrhagic Escherichia coli O157:H7 to abiotic surfaces of cooking utensils. When the cell suspension in 0.85% NaCl (about 100 cells/mL, 10 mL) was contacted with various abiotic surfaces (square pieces, 25 cm²) at 25 °C for 20 min, the number of attached cells varied depending on the types of abiotic materials. The pathogen well attached to stainless steel (about 50 cells/25 cm²), pure titanium (35 to 45 cells/25 cm²), and glass (about 20 cells/25 cm²), but little attached to aluminum foil and plastics, irrespective of strains used. Fewer cells (below 10 cells/25 cm²) attached to stainless steel, pure titanium, and glass surfaces conditioned with aseptically sliced beef (sirloin) and autoclaved beef tallow at 25 °C for 20 min, but bovine serum albumin did not reduce the number of attached cells. The cells grown at 15 °C to the stationary phase (OD660 = about 2.8) less attached to the abiotic surfaces than those grown at 25 °C and 37 °C. When we pretreated the cells at 37 °C for 2 h with 50 μM N-hexanoyl-L-homoserine lactone (HHL), the number of cells attached to stainless steel was reduced by 70%. The number of cells attached to cooking utensils seemed to change depending on types of abiotic materials, adhesion of beef tallow to abiotic surfaces, growth temperature of the pathogen, and HHL-producing bacteria.


PMID: 22515247

DOI: 10.1111/j.1750-3841.2012.02654.x

Other references

Nguyen, T.L.; Valayer, J., 1994: Congenital dilatation of the common bile duct in children. Study of a series of 52 cases. The aim of this study was to evaluate results at long-term follow-up of patients suffering from congenital dilatation of the bile duct according to the type of operative procedure. A retrospective study of 52 patients who were operated on during a...

Leizorovicz, A.; Haugh, M.C.; Mercier, C.; Boissel, J.P., 1997: Pre-hospital and hospital time delays in thrombolytic treatment in patients with suspected acute myocardial infarction: Analysis of data from the EMIP study. Objectives: To compare the components of the time delay involved in pre-hospital and hospital thrombolytic therapy in patients presenting with suspected acute myocardial infarction. Material and methods: From October 1988 to January 1992 a total o...

Krasova, N.; Poltorak, V.; Leshchenko, Z.; Karachentsev, Y.; Zagayko, A., 2002: Impaired glucose tolerance and increased lipid peroxidation in rat female offspring of mothers with insulin resistance. Diabetologia 45(Supplement 2): A 293, August

Slavnina, T.; Ivanova, R., 1984: Fraction composition of mineral phosphates in floodplain soils of the Middle Ob River area. Agrokhimiia (5) 20-26

Lapietra, G., 1974: Systemic insecticides treatments of poplar cuttings in order to prevent the attacks of Gypsonoma aceriana Dup. in a n ursery. Cellulosa e carta: 25 (5) 61-71

Anonymous, 2015: 'Grave concerns' for hard-won health visitor gains. Nursing Times 111(30-31): 5-5

Heusser C.H., 1976: Independency of fc receptor and beta 2 micro globulin. Federation Proceedings 35(3): 856

Torres, H., 1999: Biochemistry and molecular biology A southern look. FASEB Journal 13(7): A1522, April 23

Lin, Z.; Falkinham, J.O.; Tawfik, K.A.; Jeffs, P.; Bray, B.; Dubay, G.; Cox, J.E.; Schmidt, E.W., 2013: Burkholdines from Burkholderia ambifaria: antifungal agents and possible virulence factors. Burkholdines are cyclic lipopeptides with unusual antifungal potency, making them promising leads as a new class of antifungal agents. However, a recent report using knockout mutagenesis indicates that these and related compounds, such as occidiof...

Regalla, J., 1995: Serological diagnosis of contagious bovine pleuropneumonia Application and interpretation of results using complement fixation test. Revue Scientifique et Technique Office International des Epizooties 14(3): 631-644