geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

N-Glycosylation Diversity of CBHI


, : N-Glycosylation Diversity of CBHI.

Four cellobiohydrolase I (CBHI) glycoforms, namely, CBHI-A, CBHI-B, CBHI-C, and CBHI-D, were purified from the cultured broth of Penicillium decumbens JU-A1. All glycoforms had the same amino acid sequence but displayed different characteristics and biological functions. The effects of the N-glycans of the glycoforms on CBH activity were analyzed using mass spectrum data. Longer N-glycan chains at the Asn-137 of CBHI increased CBH activity. After the N-glycans were removed using site-directed mutagenesis and homologous expression in P. decumbens, the specific CBH activity of the recombinant CBHI without N-glycosylation increased by 65% compared with the wild-type CBHI with the highest specific activity. However, the activity was not stable. Only the N-glycosylation at Asn-137 can improve CBH activity by 4%. rCBHI with N-glycosylation only at Asn-47 exhibited no enzymatic activity. CBH activity was affected whether or not the protein was glycosylated, together with the N-glycosylation site and N-glycan structure. N-Glycosylation not only affects CBH activity but may also bring a new feature to a nonhydrolytic CBHI glycoform (CBHI-A). By supplementing CBHI-A to different commercial cellulase preparations, the glucose yield of lignocellulose hydrolysis increased by >2%. After treatment with a low dose (5 mg/g substrate) of CBHI-A at 5 C for 7 days, the hydrogen-bond intensity and crystalline degree of cotton fibers decreased by 17 and 34%, respectively. These results may provide new guidelines for cellulase engineering.

US$29.90


Other references

Antonogiannakis, E.; Yiannakopoulos, C.K.; Karabalis, C.; Hiotis, I., 2005: Intraoperative arthroscopic suture anchor reloading. The success of an arthroscopic procedure depends considerably on the successful and secure tying of arthroscopic knots, which reapproximate and stabilize avulsed or torn soft tissues to their original bed. Failure to perform appropriate knot tying...

Duvoisin R.C., 1975: The selection of patients. Stern, Gerald (Ed ) The Clinical Uses Of Levodopa 157p Illus University Park Press: Baltimore, Md , U S A Isbn 0-8391-0810-9 5-40

Gebhard, B., 1948: What good are health museums?. Rhode Island Medical Journal 31(12): 732, &c-732, &c

Gombos, Tímea.; Kertész, K.; Csíkos, A.; Söderhamn, U.; Söderhamn, O.; Prohászka, Zán., 2009: Nutritional form for the elderly is a reliable and valid instrument for the determination of undernutrition risk, and it is associated with health-related quality of life. Undernutrition is a common problem associated with clinical complications such as impaired immune response, reduced muscle strength, impaired wound healing, and susceptibility to infections; therefore, it is an important treatment target to reduce...

Mizuno M.; Kyotani Y.; IInuma M.; Tanaka T.; Kojima H.; Iwatsuki K., 1990: Kaempferol glycosides in asplenium scolopendrium newm. Five kaempferol glycosides in the fronds of Asplenium scolopendrium were isolated. The structures of three novel flavonol glycosides were determined to be kaempferol 3-O-.beta.-D-glucopyranosyl-(1 .fwdarw. 3)-.beta.-D-(2-O-caffeoyl)glucopyranoside...

Aaltonen, V.T., 1951: The Finnish forest types in the light of soil research.. Summarizes present information, from individual research and State surveys, on the relationship between different characteristics (physical properties, depth of the humus layer and the A horizon, and chemical and microbiological properties) of the...

Puigvert, A., 1979: Etiopathogenesis of congenital hydronephrosis. Acta Urologica Belgica 47(3): 560-570

Kelly, J.P.; Darvas, F.; Weiss, A.H., 2014: Waveform variance and latency jitter of the visual evoked potential in childhood. Recording the visual evoked potential (VEP) in young children is challenging due to movement artifacts with variable fixation or attention. This study examined the effects of latency jitter, noise, and waveform consistency on the averaging of the...

Pence, B.C.; Tsai, S.Y.; Richard, B.C., 1991: Effects of dietary fat on hepatic microsomal metabolism of 1,2-dimethylhydrazine. The present study was designed to examine the effects of different high fat diets on the liver microsomal metabolism of aminopyrine (AMP) and 1,2-dimethylhydrazine (DMH). Male Sprague-Dawley rats were fed either a low fat (5% corn oil) or high fat...

Anonymous, 1954: Crown improvement fellings.. cf. For. Abstr. 15 (No. 3423).] The girth distribution in 20-cm. classes in the Mayumbe blocks H52 and H53 is shown separately for dominants, dominated trees and shrubs, and the 12 valuable species. The successional status of the blocks is discuss...