geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Zinc transporter (ZnT)8(186-194) is an immunodominant CD8+ T cell epitope in HLA-A2+ type 1 diabetic patients


, : Zinc transporter (ZnT)8(186-194) is an immunodominant CD8+ T cell epitope in HLA-A2+ type 1 diabetic patients. Diabetologia 55(7): 2026-2031

Anti-zinc transporter (ZnT)8 autoantibodies are commonly detected in type 1 diabetic patients. We hypothesised that ZnT8 is also recognised by CD8+ T cells and aimed to identify HLA-A2 (A*2:1)-restricted epitope targets. Candidate epitopes were selected by ZnT8 plasmid DNA immunisation of HLA-A2/DQ8 transgenic mice and tested for T cell recognition in peripheral blood mononuclear cells of type 1 diabetic, type 2 diabetic and healthy participants by IFN-? enzyme-linked immunospot. White HLA-A2+ adults (83%) and children (6%) with type 1 diabetes displayed ZnT8-reactive CD8+ T cells that recognised a single ZnT8186 194 (VAANIVLTV) epitope. This ZnT8186 194-reactive fraction accounted for 5% to 53% of total ZnT8-specific CD8+ T cells. Another sequence, ZnT8153 161 (VVTGVLVYL), was recognised in 2% and 25% of type 1 diabetic adults and children, respectively. Both epitopes were type 1 diabetes-specific, being marginally recognised by type 2 diabetic and healthy participants (7-12% for ZnT8186 194, % for ZnT8153 161). Conclusions/interpretation ZnT8-reactive CD8+ T cells are predominantly directed against the ZnT8186 194 epitope and are detected in a majority of type 1 diabetic patients. The exceptional immunodominance of ZnT8186 194 may point to common environmental triggers precipitating beta cell autoimmunity.

US$19.90

PMID: 22526607

DOI: 10.1007/s00125-012-2543-z


Other references

Lindo, D.T., 1969: A painter-dentist. An alumnus with talents to spare. Medical Affairs: 34-36

Patey, A.L.; Startin, J.R.; Rowbottom, P.M.; Shearer, G., 1987: Identification of substituted hydroxypyridines and hydroxypyrazines in caramel food colourings. Chloroform-ethanol soluble components from 15 caramel colourings have been separated by gas chromatography, after trimethylsilylation. Mass spectra have been obtained on the components shown present. Ten compounds have been structurally identified...

Brochot, Céline.; Bois, Fédéric.Y., 2006: Use of a chemical probe to increase safety for human volunteers in toxicokinetic studies. To avoid interspecies extrapolation in toxicokinetics and drug development, it is convenient to directly develop human data. In that case, exposure dose should pose null or negligible risk to the exposed individual, but still be sufficiently high...

Saiga, S.; Saitoh, H.; Sabreen, S.; Tsuiki, M., 2002: Effectiveness of nutrient solution culture for detecting genetic variability in Mg concentration of orchardgrass (Dactylis glomerata L.). This study was conducted to examine the effectiveness of nutrient solution culture in detecting genetic variability of shoot Mg concentration. Six orchardgrass cultivars (HighMg-E, HighMg-M, LowEq, LowMq, Akimidori and Okamidori) were examined for...

Ivanova, G., 1978: Statistical review of catches of fish and other aquatic organisms in the World Ocean and the inland waters. Izvestiya na Instituta po Ribni Resursi - Varna, 16: 147-182

Osorio, J.-Da-S.J.rdim, P.-Da-C.G.erreiro, J.; Farias, J.-Da-S.C.ny, C., 1983: Production of meat of cattle slaughtered in cold storage conditions. 1. Estimates for bullocks and cows. Comunicado tecnico Empresa Brasileira de Pesquisa Agropecuaria Unidade de Execucao de Pesquisa de Ambito Estadual de Pelotas: 83 (12)

Han, J.F.; Qi, Q.G.; Zhang, X.M.; Zhao, Y.Y., 1988: Study of physiological effects of 2,4-epi brassinolide (BR) on the growth and development. In field trials and hydroponic studies in 1986-87 with tobacco cv. NC89 soaking the seed in 0.05 or 0.01 p.p.m. epibrassinolide for 24 h increased seed oxygen absorption, ethylene production and germination rate. Seed treatment also increased root...

Van Avery, Peter, 1965: Medical magnets. Yale Sci 39(5): 18

Setterholm, V.; Hunt, J., 1987: Method and apparatus for forming three dimensional structural components from wood fiber. Methods and apparatus for producing three-dimensional structural members having one flat side from wood fibers utilizing flow deposition of the fibers onto a support. The support includes resilient deformable mold inserts. The mold inserts are for...

Inoue, Y.; Koinuma, K.; Mochizuki, N.; Kato, A., 1989: Evaluation of Japanese corn landraces belonging to Caribbean flint for southern corn leaf blight (Bipolaris maydis) and some other practical traits. Resistance to B. maydis [Cochliobolus heterostrophus], banded leaf and sheath spot [Cercospora sorghi?], smut [Ustilago zeae] and lodging, days to mid silking, spring vigour, tiller number, stalk length and ear insertion height were studied in 376...