geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Eukaryotic gene invasion by a bacterial mobile insertion sequence element IS2 during cloning into a plasmid vector


, : Eukaryotic gene invasion by a bacterial mobile insertion sequence element IS2 during cloning into a plasmid vector. Genome Integrity 1(1): 2-2

Escherichia coli (E. coli) are commonly used as hosts for DNA cloning and sequencing. Upon transformation of E. coli with recombined vector carrying a gene of interest, the bacteria multiply the gene of interest while maintaining the integrity of its content. During the subcloning of a mouse genomic fragment into a plasmid vector, we noticed that the size of the insert increased significantly upon replication in E. coli. The sequence of the insert was determined and found to contain a novel DNA sequence within the mouse genomic insert. A BLAST search of GenBank revealed the novel sequence to be that of the Insertion Sequence 2 (IS2) element from E. coli that was likely inserted during replication in that organism. Importantly, a detailed search of GenBank shows that the IS2 is present within many eukaryotic nucleotide sequences, and in many cases, has been annotated as being part of the protein. The results of this study suggest that one must perform additional careful analysis of the sequence results using BLAST comparisons, and further verification of gene annotation before submission into the GenBank.

US$19.90

PMID: 20678256

DOI: 10.1186/2041-9414-1-2


Other references

Schenk, J., 1929: Die Suschkingans (Anser neglectus) in Ungarn. Journal fuer Ornithologie Berlin Festschr Hartert, 282-291

Fares, N.; Nader, L.; Saliba, Y.; Aftimos, G.; Gebara, V., 2011: B-type natriuretic peptide modulates the action potential and the L-type calcium current in adult rat heart muscle cells. Natriuretic peptides seem to be a potent regulator of cell Ca2+ signalling in their action on the cardiovascular system. It was therefore the aim of this study to investigate the effect(s) of B-type natriuretic peptide (BNP) on the action potentia...

Labuda, J.Z.usch, M.H.rceg, O.B.ldt, E.B.ro, D., 1979: Physiological indices of rumen fluid and the digestibility of nutrients of feed rations prepared by various technology in dairy cows. Acta zootechnica5(35): 5-17

Pepys J., 1975: Serum immuno globulins and clinical features of asthma british thoracic and tuberculosis association asthma immunology study. Tubercle 56(1): 78-79

Luna Guzman, I.; Cantwell, M.; Barrett, D.M., 1999: Fresh-cut cantaloupe: effects of CaCl2 dips and heat treatments on firmness and metabolic activity. Calcium chloride (1-5%) dips were applied to melon cylinders taken from commercially ripe (3/4 to full dip) cantaloupe melons for 1-5 min. A wound response was observed after cutting, and CO2 production was higher in untreated samples than in calc...

Guerin, C.; Moyne, M.A., 1972: Obtaining a cell line from MOPC 173 murine plasmacytoma cultivated in a medium without serum. Comptes Rendus Hebdomadaires des Seances de L'academie des Sciences. Serie D: Sciences Naturelles 275(19): 2157-2160

Pogorelov, P.N., 1938: Efficiency of mineral fertilizers for spring cereals in relation to their biological characteristics.. Spring wheat needs top dressing at the earliest stages of its development, whereas barley requires mineral fertilizers during the period of tillering.

Isk, M.; Tuncdemir, M.; Yanlmaz, A.F., 1983: Study on the control possibilities of Parthenolecanium rufulum Ckll. by Verticillium lecanii (Zimm.) Viegas. A spore suspension of Verticillium lecanii was tested in 1981 and 1982 on hazel in the Trabzon region of Turkey for the control of Parthenolecanium rufulum (Ckll.), which damaged the leaves. The pathogen at 1 x 108 spores/ml gave 47.28 and 62.3% c...

Xie, J.X.; Zhou, J.; Jia, X.X.; Liu, C.X.; Xu, H.Q.; Fang, A.S.; Wang, J.Z.; Xia, B.Y., 1980: Studies on the synthesis of anisodamine. Yao Xue Xue Bao 15(7): 403-409

Anonymous, 1990: Progress report from the period 1 January 1988-31 December 1989. Arbeiten aus dem Paul-Ehrlich-Institut Zu Frankfurt A.M: 1-179