+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Immunotoxin targeting CD133(+) breast carcinoma cells

, : Immunotoxin targeting CD133(+) breast carcinoma cells. Drug Delivery and Translational Research 3(2): 195-204

CD133 expression enriches for tumor-initiating cells and is a negative prognostic factor in numerous cancers. We previously developed an immunotoxin against CD133 by fusing a gene fragment encoding the scFv portion of an anti-CD133 antibody to a gene fragment encoding deimmunized PE38KDEL. The resulting fusion protein, dCD133KDEL, demonstrated potent antitumor activity following intratumoral delivery into head neck cell carcinoma xenografts. However, the efficacy against other tumors and the tolerability of systemic administration remained unclear. The purpose of this study was to evaluate the tolerability and efficacy of dCD133KDEL in a systemic human breast carcinoma model. Time course viability studies showed that dCD133KDEL selectively inhibited MDA-MB-231 ductal breast carcinoma cells that contained a minority CD133+ subpopulation, implicating CD133+ cells as a source for self-renewal within this cell line. Furthermore, systemic administration of dCD133KDEL caused regression or inhibition of tumor growth in mice bearing an intrasplenic MDA-MB-231 tumor challenge as a model for metastatic disease. In the same model, combined therapy with dCD133KDEL and another immunotoxin designed to target the bulk tumor mass was the most effective therapy, supporting the idea that such combination therapies might better address tumor heterogeneity. dCD133KDEL shows promise as a therapeutic agent and as a biologic tool to study cancer stem cells.


PMID: 25787984

DOI: 10.1007/s13346-012-0066-2

Other references

Bourdeau, D.; Toudic, P.; Gouez, J-François.; Lannurien, A.; Mayer, C.; Myre, A-Florence.; Alibar, B.; Gaempiokilly, M., 2011: Management of the hospitalized patient with acute sickle cell crisis. Revue de L'infirmiere: 18-19

Yuan, H.; Wilson, C.M.; Xia, J.; Doyle, S.L.; Li, S.; Fales, A.M.; Liu, Y.; Ozaki, E.; Mulfaul, K.; Hanna, G.; Palmer, G.M.; Wang, L.V.; Grant, G.A.; Vo-Dinh, T., 2014: Plasmonics-enhanced and optically modulated delivery of gold nanostars into brain tumor. Plasmonics-active gold nanostars exhibiting strong imaging contrast and efficient photothermal transduction were synthesized for a novel pulsed laser-modulated plasmonics-enhanced brain tumor microvascular permeabilization. We demonstrate a select...

Kust, G.S.; Andreeva, O.V., 2000: International Conference on Land Degradation and Desertification. Pochvovedenie (9): 1153-1158, September

Sobczak, Z., 1961: Feeding value of maize silage and its dependence on the protein supplements for dairy cattle. In a trial of Latin square design 12 Black Pied Lowland cows of 500 kg liveweight were given 3 rations for periods of 20 days and in the second 10 days milk yield and percentage fat were estimated. The rations had lucerne hay, dried brewer's...

Donnelly, H.; Connor, S.; Quirk, J., 2016: Central pontine myelinolysis secondary to hyperglycaemia. NlmCategory="UNASSIGNED">Central pontine myelinolysis is characterised by focal osmotic demyelination within the pons. Its clinical presentation varies, but may include acute paralysis, dysarthria and dysphagia. The cause is tradition...

Gol'dina, I.R.; Nadeenko, V.G.; Saĭchenko, S.P.; D'iachenko, O.Z.; Lenchenko, V.G., 1984: Sanitary-toxicological evaluation of manganese intake from drinking water. Gigiena i Sanitariia: 80-81

Girard, K.K.; Sinha, N., 2006: Cranberry, blueberry, currant, and gooseberry. Information on the cultivation/production, physicochemical properties and nutritional and health-promoting properties of cranberries, blueberries, currants (Ribes sativum [Ribes rubrum]) and gooseberries (R. grossularia [Ribes uva-crispa]) are pre...

Kondo, N.; Endo, S., 1988: Studies on fruit recognizing visual sensor (Part 3) - effectiveness of the most suitable wavelength bands in the field. Part 2 determined the most suitable wavelength bands for using spectral reflectance to discriminate between fruit and leaves under laboratory conditions. This paper describes use of a visual sensor in the field. The discrimination achieved between...

Beer, J.W.; Clarkin, K.L.; Salas, G. de las; Glover, N.L., 1981: A case study of traditional agro-forestry practices in a wet tropical zone; the La Suiza project. Simposio internacional sobre las ciencias forestales y su contribucion al desarrollo de la America Tropical, San Jose, Costa Rica, 1979 Memorias: 191-209

Delmotte, A.; Delmotte, N.; Emond, M., 1960: Reversal of the inhibition of the bactericidal and fungicidal action of phenylmercuric borate and sodium ethylmercurithiosalicylate in thioglycolate media by means of sodium laurylsulfate and sodium tetradecylsulfate. Therapie 15: 134-142