geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A field-deployable colorimetric bioassay for the rapid and specific detection of ribosomal RNA


, : A field-deployable colorimetric bioassay for the rapid and specific detection of ribosomal RNA. Biosensors & Bioelectronics 52: 433-437

Rapid and specific on-site detection of disease-causing or toxin-producing organisms is essential to public health and safety. Many molecular recognition methods target ribosomal RNA sequences due to their specificity and abundance in the cell. In this work RNA targets were identified and quantified using a colorimetric bioassay. Peptide nucleic acid (PNA) probes were used to capture RNA targets, and a micrococcal nuclease digestion was performed to remove all non-target nucleic acids, including single base mismatches flanked by adenines or uracils. Perfectly-matched PNA-RNA hybrids remained intact and were detected using the symmetrical cyanine dye 3,3 -diethylthiadicarbocyanine iodide (DiSC2). Assay applicability to complex samples was demonstrated using mixtures containing RNA sequences from two related, harmful algal bloom-causing Alexandrium species. Target RNA was detected even in mixtures with mismatched sequences in excess of the perfect match. The fieldability of the assay was tested with a portable two-wavelength colorimeter developed to quantify the dye-indicated hybridization signal. The colorimeter sensing performance was shown to be comparable to a laboratory spectrophotometer. This quick, inexpensive and robust system has the potential to replace laborious identification schemes in field environments. Rapid RNA hybridization is achieved with PNA probes and a symmetrical cyanine dye Single base-mismatched RNA are digested off the probe with micrococcal nuclease Unamplified target RNA is quantified in mixtures of related non-target sequences A handheld colorimeter was designed to make the assay field-compatible The colorimeter performance is comparable to a laboratory spectrophotometer.

US$19.90

PMID: 22749775

DOI: 10.1016/j.bios.2012.05.039


Other references

Jones, K.M.; Mulkern, R.V.; Schwartz, R.B.; Oshio, K.; Barnes, P.D.; Jolesz, F.A., 1992: Fast spin-echo MR imaging of the brain and spine: current concepts. Ajr. American Journal of Roentgenology 158(6): 1313-1320

Jung, Y.; Lee, S-Ho.; Jennings, A.T.; Agarwal, R., 2008: Core-shell heterostructured phase change nanowire multistate memory. Phase-change memory, which switches reversibly between crystalline and amorphous phases, is promising for next generation data-storage devices. In this work, we present a novel, nonbinary data-storage device using core-shell nanowires to significa...

Ahmeda, A.F.; Rae, M.G.; A.O.aibi, M.F.; Anweigi, L.M.; Johns, E.J., 2016: Effect of tempol and tempol plus catalase on intra-renal haemodynamics in spontaneously hypertensive stroke-prone (SHSP) and Wistar rats. NlmCategory="UNASSIGNED">Vasoconstriction within the renal medulla contributes to the development of hypertension. This study investigated the role of reactive oxygen species (ROS) in regulating renal medullary and cortical blood perf...

Shiau, L.-Jian; Chen, M.-Te; Huh, C.-An; Yamamoto, M.; Yokoyama, Y., 2012: Insolation and cross-hemispheric controls on Australian monsoon variability over the past 180?ka new evidence from offshore southeastern Papua New Guinea. The Australian summer monsoon (ASM) is primarily a synoptic feature determined by the differential thermal response to solar insolation between the Australian continent and the western Pacific warm pool (WPWP) in austral summer. Little is known ab...

Chasey, W.C.; Wyrick, W., 1970: Effect of a gross motor developmental program on form perception skills of educable mentally retarded children. Research Quarterly 41(3): 345-352

Orlow, S.J.; Silvers, W.K.; Zhou, B.K.ng; Mintz, B., 1998: Comparative decreases in tyrosinase, TRP-1, TRP-2, and Pmel 17/silver antigenic proteins and melanotic to amelanotic stages of syngeneic mouse cutaneous melanomas and metastases. Malignant cutaneous melanomas and metastases were taken directly from in situ lesions of genetically identical (C57BL/6 strain) Tyr-SV40E transgenic mice, and samples were analyzed by Western immunoblotting with antisera specific for the COOH term...

Narayanan, D.; Marchetti, P.; McHugh, A.; Barth, J.H., 2013: Changing practice of diabetes testing by primary care in West Yorkshire. In recent years there have been several major changes to the diagnosis and management of diabetes mellitus. The World Health Organisation (WHO) recommended the use of haemoglobin A<SUB>1C</SUB> as a diagnostic marker of diabetes in 201...

Hartsuck, J.M.; Johnson, J.E.; Moore, F.D., 1969: Potassium in bone: evidence for a nonexchangeable fraction. Comparison of the specific activity of 42K in bone and plasma provides direct evidence for the existence of non-exchangeable K in the skeleton of man and the dog. Serial sampling suggested that the exchangeable fraction was exchanged within 24 h.

Crawford, T.N., 2015: Poor retention in care one-year after viral suppression: a significant predictor of viral rebound. Optimal retention in care should be continuously monitored even after suppression to prevent the risk of viral rebound. The purpose of this study is to assess the association between retention in care and viral rebound 12 months after viral suppre...

Panigrahi, M.Kumar.; Kumar, D.; Mehta, A.; Suryavanshi, M.; Bhurani, D.; Saikia, K.Kumar., 2017: Novel e8a2 BCR/ABL Fusion Transcript in Case of a Myeloproliferative Neoplasm. Main objective of this work was to confirm the occurrence of rare BCR-ABL fusion variant involving the a2 region of the ABL gene and e8 of BCR gene in a patient of Myeloproliferative neoplasm positive for t(9;22) translocation but negative for com...