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Osteoblastogenesis from synovial fluid-derived cells is related to the type and severity of juvenile idiopathic arthritis


, : Osteoblastogenesis from synovial fluid-derived cells is related to the type and severity of juvenile idiopathic arthritis. Arthritis Research & Therapy 14(3): R139-R139

Juvenile idiopathic arthritis (JIA) is characterized by synovial inflammation, followed by hyperplastic changes of the synovium, and destruction of articular cartilage along with underlying bone. This hyperplastic process is the result of inflammation-induced activation of NF-kappaB, which may be accompanied by decreased osteogenic differentiation of synovial mesenchymal progenitors and contribute to bone resorption. We aimed to explore osteoblast differentiation of synovial fluid (SF)-derived mesenchymal progenitors and correlate it with intensity of inflammation in patients with JIA. Peripheral blood from 18 patients with oligoarticular (o)JIA, 22 patients with polyarticular (p)JIA and 18 controls was collected along with SF from 18 patients with oJIA and 9 patients with pJIA. SF-derived cells were cultured to assess osteoblastogenesis, using alkaline phosphatase histochemical staining and colorimetric activity assay. The expression of osteoblast-related genes, Runt-related transcription factor 2 (Runx2), Osteoprotegerin (OPG), Receptor activator of nuclear factor kappaB ligand (RANKL) and arthritis-related cytokine/chemokine genes, Tumor necrosis factor alpha (TNF-alpha, Fas, Fas ligand (FasL), Interleukin (IL)-1beta, IL-4, IL-6, IL-17, IL-18, CC chemokine ligand (CCL)-2, CCL3, CCL4 was evaluated. Osteoblastogenesis was correlated with systemic and local inflammatory indicators. Expression of osteoblast genes was also analyzed in peripheral blood mononuclear cells (PBMC) and total SF-derived cells from patients with JIA. Additionally, we assessed the inhibitory effect of SF from patients with JIA on differentiation of human bone marrow (hBM)-derived osteoblasts. Osteoblastogenesis from SF-derived progenitors was decreased in patients with pJIA compared to those with oJIA. Osteoblastogenesis from primary SF-derived cells negatively correlated with erythrocyte sedimentation rate (rho=-.391, P=.5), C-reactive protein concentration (rho =-.527, P<.1) and synovial concentration of IL-17 (rho= -.552, P=.1). SF-derived osteoblasts from pJIA patients expressed more CCL2 and CCL3 genes than in oJIA (P=.4 and P=.3, respectively; Mann-Whitney test). Expression of Fas was significantly higher in osteoblasts from patients with pJIA than those with oJIA (P=.3, Mann-Whitney test). SF-derived cells from patients with pJIA expressed higher levels of RANKL than in oJIA (P=.5, Mann-Whitney test). PBMCs from patients with JIA expressed less OPG than healthy control patients (P=.5, Kruskal-Wallis test). SF from all tested JIA patients inhibited differentiation of hBM-derived osteoblasts (P=.4, Kruskal-Wallis test). Osteoblast differentiation was decreased in patients with severe forms of JIA and accompanied by altered cytokine/chemokine expression pattern. Development of therapeutic interventions targeting synovial mesenchymal or osteoblast lineage cells in JIA would contribute to alleviating both bone destruction and inflammation in severe forms of the disease.

US$19.90

PMID: 22687048

DOI: 10.1186/ar3872


Other references

Auer, Carol, 2008: Ecological Risk Assessment and Regulation for Genetically-Modified Ornamental Plants. Critical Reviews in Plant Sciences 27(4): 255-271

Lepper, A.W.; Haig, D.A.; Wilcox, J., 1969: Cellular pathology of calves experimentally infected with bovine herpes mammillitis virus. The production and release of complete virus particles, the degree of epidermal and sebaceous tissue damage and the febrile response appeared to be at a maximum 8 days after inoculation. Coagulative scab material was observed to contain large numb...

Gorecka E., 1973: Development of Ore Mineralization in the Northeastern Part of the Cracow-Silesian Area. Metallogeny, lead-zinc ores, paragenesis, hydrothermal activity, Poland.

Arsuffi, A.; Lazzari, M.; Meterangelis, G., 1987: Aspects of pseudomutuality between the schizophrenic patient, his family and the patient care team. Minerva Psichiatrica 28(1): 19-24

Fiorelli, G.; Melsen, B.; Modica, C., 2001: Differentiated orthodontic mechanics for dental midline correction. Journal of Clinical Orthodontics 35(4): 239-244

Oliveira, S.L. de; Diniz, D.B.; Amaya Farfan, J., 2002: Metabolic changes induced by energy restriction and vitamin E supplementation in exercised rats. The effects of energy (carbohydrate) restriction and vitamin E supplementation on some exercise-induced metabolic modifications in rats were studied. Male Wistar, 11-week-old rats were fed control, or restricted amounts of a modified control, or c...

Peña-Perez, L.Manuel.; Pedraza-Ortega, J.Carlos.; Ramos-Arreguin, J.Manuel.; Arriaga, S.Tovar.; Fernandez, M.Antonio.Aceves.; Becerra, L.Omar.; Hurtado, E.Gorrostieta.; Vargas-Soto, J.Emilio., 2013: Alignment of the measurement scale mark during immersion hydrometer calibration using an image processing system. The present work presents an improved method to align the measurement scale mark in an immersion hydrometer calibration system of CENAM, the National Metrology Institute (NMI) of Mexico, The proposed method uses a vision system to align the scale...

Fumarola, D.; Pasquetto, N.; Telesforo, P.; Donati, M.B., 1975: Studies on the clotting mechanism of Limulus polyphemus. Thrombosis Research 7(3): 401-408

Yuan, L.; Zhang, X.; Xiao, N.; Dai, L.; Chen, W.; Jia, C.; Zhao, R.; Hemmingsen, S.M.; Dai, H., 2006: Identification of a WSSV neutralizing scFv antibody by phage display technology and in vitro screening. White spot syndrome virus (WSSV) is one of the most significant viral pathogens causing high mortality and economic damage in shrimp aquaculture. Although intensive efforts were undertaken to detect and characterize WSSV infection in shrimp durin...

Ringe, D., 1995: Structure-aided drug design: crystallography and computational approaches. Ideally, a drug that effectively attacks a specific target will be efficacious with few or no side effects. Historically, drug development has relied on a structure-activity sequential (trial-and-error) approach, testing many compounds and observi...