geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An intercellular pathway for glucose transport into mouse oocytes


, : An intercellular pathway for glucose transport into mouse oocytes. American Journal of Physiology. Endocrinology and Metabolism 302(12): E1511-8

Glucose is an essential nutrient for mammalian cells. Emerging evidence suggests that glucose within the oocyte regulates meiotic maturation. However, it remains controversial as to whether, and if so how, glucose enters oocytes within cumulus-oocyte complexes (COCs). We used a fluorescent glucose derivative (6-NBDG) to trace glucose transport within live mouse COCs and employed inhibitors of glucose transporters (GLUTs) and gap junction proteins to examine their distinct roles in glucose uptake by cumulus cells and the oocyte. We showed that fluorescent glucose enters both cumulus-enclosed and denuded oocytes. Treating COCs with GLUT inhibitors leads to simultaneous decreases in glucose uptake in cumulus cells and the surrounded oocyte but no effect on denuded oocytes. Pharmacological blockade of of gap junctions between the oocyte and cumulus cells significantly inhibited fluorescent glucose transport to oocytes. Moreover, we find that both in vivo hyperglycemic environment and in vitro high-glucose culture increase free glucose levels in oocytes via gap junctional channels. These findings reveal an intercellular pathway for glucose transport into oocytes: glucose is taken up by cumulus cells via the GLUT system and then transferred into the oocyte through gap junctions. This intercellular pathway may partly mediate the effects of high-glucose condition on oocyte quality.

US$19.90

PMID: 22473000

DOI: 10.1152/ajpendo.00016.2012


Other references

Miller, G.G.; Pollack, M.S.; Nell, L.J.; Thomas, J.W., 1987: Insulin-specific human T cells. Epitope specificity, major histocompatibility complex restriction, and alloreactivity to a diabetes-associated haplotype. T cells from an insulin-treated diabetic (ML, HLA DR1, w6) were stimulated in vitro with insulin, cloned at limiting dilution, and examined for their fine specificity and genetic restriction. T cell lines (TCL) derived from beef insulin stimulatio...

Iidaka, T.; Tsukamoto, T.; Totsuka, Y.; Hirata, A.; Sakai, H.; Shirai, N.; Yamamoto, M.; Wakabayashi, K.; Yanai, T.; Masegi, T.; Donehower, L.A.; Tatematsu, M., 2004: Lack of elevated liver carcinogenicity of aminophenylnorharman in p53-deficient mice. The hepatocarcinogenic potential of 9-(4'-aminophenyl)-9H-pyrido(3,4-b)indole (aminophenylnorharman, APNH) was investigated using male and female p53 deficient mice. Incidence of oval cell hyperplasia was 2/14 (14.3%), 14/23 (60.9%), and 2/10...

Ishino K.; Ubuka T., 1991: Isoelectric focusing of proteins in slab polyacrylamide gel prepared on cellophane sheet. A method of isoelectric focusing in slab polyacrylamide gel prepared on a sheet of cellophane was developed. A slab polyacrylamide gel containing 2% carrier ampholyte was prepared on a cellophane sheet placed on a plastic film. The gel prepared on...

Wasserman, B.P.; Hultin, H.O.; Jacobson, B.S., 1980: High-yield method for immobilization of enzymes. Two types of polyethylenimine-coated glass microbeads (13-44 micrometer) were synthesized and used for the immobilization of glucose oxidase from Aspergillus niger and catalase from A. niger and beef liver. The two types of beads were distinguisha...

Lippiello L.; Mitrovic D.; Mankin H.J., 1977: Effect of prostaglandins on metabolism of isolated chondrocytes. Arthritis & Rheumatism 20(1): 125

Roundy, K.; Cully, J.A.; Stanley, M.A.; Veazey, C.; Souchek, J.; Wray, N.P.; Kunik, M.E., 2005: Are anxiety and depression addressed in primary care patients with chronic obstructive pulmonary disease? A chart review. Screening for mental illness in primary care is widely recommended, but little is known about the evaluation, treatment, and long-term management processes that follow screening. The aim of this study was to examine and describe the quality of men...

Foltýnová, V., 1986: Evaluation of prognostic factors in Hodgkin's disease. Experience of the comprehensive rationale team in treating patients with Hodgkin's disease. Casopis Lekaru Ceskych 125(22): 684-690

Degelaen J.P.; Pham P.; Blout E.R., 1984: Synthesis and a structural study of an ion binding cyclic peptide analog of valinomycin cyclo tri l alanylglycyl d phenylalanyl l prolyl. The synthesis and the 270 MHz NMR investigation of an ion binding cyclic peptide analog of valinomycin [cyclo(L-Ala-Gly-D-Phe-L-Pro)3 (c-(AGPhP)3)] and its cation complexes are reported. In CDCl3 the peptide exists in a C3 symmetric propeller stru...

Nelson, V.; Tallontire, A.; Collinson, C., 2002: Assessing the benefits of ethical trade schemes for forest dependent people: comparative experience from Peru and Ecuador. In this paper we consider the social impact of ethical trade schemes, particularly the kinds of benefits ethical trade provides for smallholder farmers and their livelihoods and for sustainable forestry. We focus on two cases, which are linked to...

Stalcer, J., 2001: Beetup-herbicides, indispensable for oppression of weed in sugar beet. Sugarbeet has been produced in Slovenia for many years. During this period, we have collected great experiences. Sugarbeet is, technologically, a very sophisticated agricultural crop which demands accuracy and consistency with protection against w...