+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Fabrication of an inexpensive, implantable cooling device for reversible brain deactivation in animals ranging from rodents to primates

, : Fabrication of an inexpensive, implantable cooling device for reversible brain deactivation in animals ranging from rodents to primates. Journal of Neurophysiology 107(12): 3543-3558

We have developed a compact and lightweight microfluidic cooling device to reversibly deactivate one or more areas of the neocortex to examine its functional macrocircuitry as well as behavioral and cortical plasticity. The device, which we term the "cooling chip," consists of thin silicone tubing (through which chilled ethanol is circulated) embedded in mechanically compliant polydimethylsiloxane (PDMS). PDMS is tailored to compact device dimensions (as small as 21 mm3) that precisely accommodate the geometry of the targeted cortical area. The biocompatible design makes it suitable for both acute preparations and chronic implantation for long-term behavioral studies. The cooling chip accommodates an in-cortex microthermocouple measuring local cortical temperature. A microelectrode may be used to record simultaneous neural responses at the same location. Cortex temperature is controlled by computer regulation of the coolant flow, which can achieve a localized cortical temperature drop from 37 to 2°C in less than 3 min and maintain target temperature to within ±.3°C indefinitely. Here we describe cooling chip fabrication and performance in mediating cessation of neural signaling in acute preparations of rodents, ferrets, and primates.


PMID: 22402651

DOI: 10.1152/jn.01101.2011

Other references

Butler, Declan, 1997: CNRS gets first woman director general. Last week, physicist Catherine Brechignac became the first woman director general of France's Centre National de la Recherche Scientifique (CNRS). Since November 1995, 51-year-old Brechignac has headed the center's Department of Physical...

Kruglov, Y.V.; Kobyshcha, Y.V.; Salyuk, T.; Varetska, O.; Shakarishvili, A.; Saldanha, V.P., 2008: The most severe HIV epidemic in Europe: Ukraine's national HIV prevalence estimates for 2007. Objective: To revise the national HIV estimates and quantify the magnitude of the HIV epidemic in Ukraine at the end of 2007.Design: Internationally recommended methods-the Workbook and Spectrum-were employed to generate the estimates. This enabl...

Casini, G.; Brecha, N.C., 1992: Colocalization of vasoactive intestinal polypeptide and GABA immunoreactivities in a population of wide-field amacrine cells in the rabbit retina. Vasoactive intestinal polypeptide (VIP) immunoreactive (IR) neurons in the rabbit retina constitute a population of wide-field amacrine cells. To better define this cell population, we examined the coexpression of VIP with other putative retinal t...

Laufle J.C.; Pauley G.B.; Shepard M.F., 1986: Species profiles life histories and environmental requirements of coastal fishes and invertebrates pacific northwest coho salmon oncorhynchus kisutch. U S Fish & Wildlife Service Biological Report (8211 PART 48): I-VI, 1-18

Viermann, H., 1965: Statutory regulations in ice cream control. Some standards are given for the manufacture of ice cream in 11 regions of West Germany. Minimum heat treatment standards are required in 3 regions, but specifications are only given for Berlin, where the ice cream mix must be heated to 65 degrees...

Sodre, G. da S.; Marchini, L.C.; Zucchi, O.L.A.D.; Nascimento Filho, V.F.; Otsuk, I.P.; Moreti, A.C. de C.C., 2007: Determination of chemical elements in Africanized Apis mellifera (Hymenoptera: Apidae) honey samples from the State of Piaui, Brazil. Honey has been used as a food since the most remote times, which is appreciated for its characteristic flavour, considerable nutritional value and medicinal properties; however, little information exists about its chemical element composition, whi...

Shigeta, Y.; Kikkawa, R.; Izumi, K., 1969: Serum ketone bodies: interpretation of the test results. Nihon Rinsho. Japanese Journal of Clinical Medicine: Suppl:805-10

Schwangart F., 1929: Notes on the biological Control of the Vine-moths. In connection with the biological control of the vine-moths, Hb., and Polychrosis botrana, Schiff.] in Germany, it is pointed out that the vineyards are small so that all parts of them are accessible to parasites coming from alternative hosts on o...

Levine, D.; Even-Chen, Z.; Lipets, I.; Pritulo, O.A.; Svyatenko, T.V.; Andrashko, Y.; Lebwohl, M.; Gottlieb, A., 2010: Pilot, multicenter, double-blind, randomized placebo-controlled bilateral comparative study of a combination of calcipotriene and nicotinamide for the treatment of psoriasis. Calcipotriene has limited efficacy in treating psoriasis. By inhibiting proinflammatory cytokines such as interleukin-12, interleukin-23, and tumor necrosis factor-alfa, nicotinamide may enhance the efficacy of calcipotriene therapy when used in c...

Monod, Th, 1932: Uber drei indopanfische Cypridiniden und zwei in Ostraceden lebende Krebstiere. [long dash]Studies of Cypridina hirsuta*, C. vanhoffeni*, and C. asymmetrica*. A species* of Cyproniscus and a choniostomatid larva, perhaps Sphaeronollopsis (?) sp., were found parasitic in Cypridina vanhoffeni.