geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Hsp9 regulates O-linked beta-N-acetylglucosamine transferase a novel mechanism of modulation of protein O-linked beta-N-acetylglucosamine modification in endothelial cells


, : Hsp9 regulates O-linked beta-N-acetylglucosamine transferase a novel mechanism of modulation of protein O-linked beta-N-acetylglucosamine modification in endothelial cells.

O-linked β-N-acetylglucosamine (O-GlcNAc) modification of proteins is involved in many important cellular processes. Increased O-GlcNAc has been implicated in major diseases, such as diabetes and its complications and cardiovascular and neurodegenerative diseases. Recently, we reported that O-GlcNAc modification occurs in the proteasome and serves to inhibit proteasome function by blocking the ATPase activity in the 19S regulatory cap, explaining, at least in part, the adverse effects of O-GlcNAc modification and suggesting that downregulating O-GlcNAc might be important in the treatment of human diseases. In this study, we report on a novel mechanism to modulate cellular O-GlcNAc modification, namely through heat shock protein 9 (Hsp9) inhibition. We observed that O-linked β-N-acetylglucosamine transferase (OGT) interacts with the tetratricopeptide repeat binding site of Hsp9. Inhibition of Hsp9 by its specific inhibitors, radicicol or 17-N-allylamino-17-demethoxygeldanamycin, destabilized OGT in primary endothelial cell cultures and enhanced its degradation by the proteasome. Furthermore, Hsp9 inhibition downregulated O-GlcNAc protein modifications and attenuated the high glucose-induced increase in O-GlcNAc protein modification, including high glucose-induced increase in endothelial or type 3 isoform of nitric oxide synthase (eNOS) O-GlcNAcylation. These results suggest that Hsp9 is involved in the regulation of OGT and O-GlcNAc modification and that Hsp9 inhibitors might be used to modulate O-GlcNAc modification and reverse its adverse effects in human diseases.

US$29.90


Other references

Bisping, B.; Baumann, U.; Simmering, R., 1996: Effects of immobilization on polyol production by Pichia farinosa. Wijffels, R H [Editor], Buitelaar, R M [Editor], Bucke, C [Editor], Tramper, J [Editor] Progress in Biotechnology, Vol 11; Immobilized cells: Basics and applications 395-401

Reichholf, J., 1983: Reagieren Bestande des Hermelins Mustela erminea auf Schwankungen der Wuhlmaushaufigkeit?. Saugetierkundliche Mitteilungen, 311: 69-72

Belitz, 1986: Correlation-induced reentry in impure superconductors. Physical Review Letters 56(11): 1175-1178

Louboutin J.P.; Elie B.; Bouissiere J.; Guiheneuc P.; Feve J.R., 1990: Somatosensory evoked potentials seps in patients with igm monoclonal proteins and peripheral neuropathy. Electroencephalography & Clinical Neurophysiology 75(1): S84

Wiberg, K.B.; Zilm, K.W., 2001: 19F NMR chemical shifts. 1. Aliphatic fluorides. The (19)F NMR shielding for the alkyl fluorides from methyl fluoride to tert-butyl fluoride has been calculated using IGAIM and has been separated into the contribution from each of the molecular orbitals. The relatively large change in fluorine s...

Rose S.L.; Youngberg C.T., 1981: Tripartite associations in snowbrush ceanothus velutinus effect of vesicular arbuscular mycorrhizae on growth nodulation and nitrogen fixation. Symbiotic associations were established between N-fixing nonleguminous (actinorrhizal) snowbrush (C. velutinus Dougl.) seedlings and 2 categories of microorganisms: vesicular-arbuscular (VA) mycorrhizal fungi and a filamentous actinomycete capable...

Delfini, C.; Polchi, P.; Izzi, T.; Nicolini, G.; Paradisi, O.; Lucarelli, G., 1985: Bone marrow donors other than HLA genotypically identical siblings for patients with thalassemia. This report describes the successful bone marrow transplantation of three children with thalassemia who received bone marrow, one from an HLA identical but mixed lymphocyte culture-reactive sibling, the other two from an HLA phenotypically identic...

Falkenberg, Mårten.; Cronqvist, J.; Malina, M., 2012: Early stent fracture and collapse of a Zenith TX2 thoracic stent graft used for traumatic aortic rupture. Structural failure and collapse of thoracic stent grafts may cause fatal complications that are easily overlooked on follow-up imaging. A young man with multiple injuries from a motorcycle crash was treated with endografting for an aortic transect...

Aloia, M.S.; Arnedt, J.Todd.; Stanchina, M.; Millman, R.P., 2007: How early in treatment is PAP adherence established? Revisiting night-to-night variability. Obstructive sleep apnea (OSA) is a serious disorder with significant daytime consequences. Treatment for OSA most commonly takes the form of positive airway pressure (PAP). Although effective, PAP adherence is often below expectations. Previous st...

Bayart F.; Turlot J C., 1984: Organization of vocal communication in 2 species of macaques primates cercopithecidae macaca fascicularis java and macaca tonkeana celebes indonesia. De Haro, A And X Espadaler (Ed ) Processus D'acquisition Precoce: Les Communications, Colloque International D'ethologie, S F E C A (Societe Fracaise Pour L'etude Du Comportement Animal); (Processes Of Early Acquisition: Communications, International Colloquium on Ethology, French Society For The Study Of Animal Behavior), Barcelona, Spain, Apr 26-28, L984 497p Universitat Autonoma De Barcelona: Barcelona, Spain Illus Maps Paper : 161-168