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Modulation of ceramide synthase activity via dimerization


, : Modulation of ceramide synthase activity via dimerization. Journal of Biological Chemistry 287(25): 21025-21033

Ceramide, the backbone of all sphingolipids, is synthesized by a family of ceramide synthases (CerS) that each use acyl-CoAs of defined chain length for N-acylation of the sphingoid long chain base. CerS mRNA expression and enzymatic activity do not always correlate with the sphingolipid acyl chain composition of a particular tissue, suggesting post-translational mechanism(s) of regulation of CerS activity. We now demonstrate that CerS activity can be modulated by dimer formation. Under suitable conditions, high M(r) CerS complexes can be detected by Western blotting, and various CerS co-immunoprecipitate. CerS5 activity is inhibited in a dominant-negative fashion by co-expression with catalytically inactive CerS5, and CerS2 activity is enhanced by co-expression with a catalytically active form of CerS5 or CerS6. In a constitutive heterodimer comprising CerS5 and CerS2, the activity of CerS2 depends on the catalytic activity of CerS5. Finally, CerS dimers are formed upon rapid stimulation of ceramide synthesis by curcumin. Together, these data demonstrate that ceramide synthesis can be regulated by the formation of CerS dimers and suggest a novel way to generate the acyl chain composition of ceramide (and downstream sphingolipids), which may depend on the interaction of CerS with each other.

US$19.90

PMID: 22539345

DOI: 10.1074/jbc.M112.363580


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Martineau, A.R.; Wilkinson, K.A.; Newton, S.M., 2007: IFN-g- and TNF-Independent Vitamin D-Inducible Human Suppression of Mycobacteria: The Role of Cathelicidin LL-37. Journal of Immunology 178(11): 90-8

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Argème, M.; Mambrini, A.; Lebreuil, G.; Guérinel, G., 1970: Dissection of the muscle of Treitz. Comptes Rendus de L'association des Anatomistes 147: 76-86

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Anderson Ann, 1971: An analysis of supposed fish trails from interglacial sediments in the Dwyka Series, near Vryheid, Natal. International Gondwana Symposium: ges 637-647. 1971.

Mehdizadeh, M.; Ahmadi, N.; Jamshidi, M., 2016: Evaluation of the relationship between mandibular third molar and mandibular canal by different algorithms of cone-beam computed tomography. Exact location of the inferior alveolar nerve (IAN) bundle is very important. The aim of this study is to evaluate the relationship between the mandibular third molar and the mandibular canal by cone-beam computed tomography. This was a cross-sect...

Bakx, P.A.; Baggen, M.G.; Veen, H.F.; van den Ingh, H.F., 1993: Metastases in the thyroid gland as cause of thyroid swelling. Nederlands Tijdschrift Voor Geneeskunde 137(18): 930-931