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USP9X Modulates mTOR Activity


, : USP9X Modulates mTOR Activity.

The mammalian target of rapamycin (mTOR) is an atypical serine/threonine kinase that responds to extracellular environment to regulate a number of cellular processes. These include cell growth, proliferation, and differentiation. Although both kinase-dependent and -independent functions of mTOR are known to be critical modulators of muscle cell differentiation and regeneration, the signaling mechanisms regulating mTOR activity during differentiation are still unclear. In this study we identify a novel mTOR interacting protein, the ubiquitin-specific protease USP9X, which acts as a negative regulator of mTOR activity and muscle differentiation. USP9X can co-immunoprecipitate mTOR with both Raptor and Rictor, components of mTOR complexes 1 and 2 (mTORC1 and -2), respectively, suggesting that it is present in both mTOR complexes. Knockdown of USP9X leads to increased mTORC1 activity in response to growth factor stimulation. Interestingly, upon initiation of differentiation of C2C12 mouse skeletal myoblasts, knockdown of USP9X increases mTORC2 activity. This increase in mTORC2 activity is accompanied by accelerated differentiation of myoblasts into myotubes. Taken together, our data describe the identification of the deubiquitinase USP9X as a novel mTORC1 and -2 binding partner that negatively regulates mTOR activity and skeletal muscle differentiation.

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Other references

Anon, 1968: Meeting of the 2nd internationales symposium pharmazeutische chemie international symposium of pharmaceutical chemistry section on the chemo therapy of parasite infections muenster germany july 22 26 1968. Deutsche Apotheker Zeitung 108(33): 1198-1200

Apeland, J., 1974: Storage quality of carrots after different methods of harvesting. In 4-year trials carrots, cv. Nantes, were harvested either by hand or mechanically and their quality was compared after 6-7 months' storage at 0 deg C. Mechanical harvesting reduced storage quality as indicated by average marketable crop by...

Anonymous, 2015: CQC identifies district nursing shortages. Nursing Times 110(41): 5-5

Griffin, J.P.; Griffin, T.D., 1993: The economic implications of therapeutic conservatism. We review the pattern of prescribing medicines in the United Kingdom (UK) and compare it with that in other European markets. The prescribing of medicines in Britain has always been more conservative than in other major European markets such as Fr...

Hosni, H.A.; Hegazy, A.K., 1996: Contribution to the flora of Asir, Saudi Arabia. 218 species belonging to 48 families from the Asir region (Saudi Arabia) are listed. Notes on ecology and geographical distributions are also provided. A new combination is proposed: Tribulus pentandrus var. acanthopterus (Kralik) H. Hosni. 12 spe...

Goret, F.; Joubert, L., 1955: An outbreak of Corynebactermm pyogenes infection in pigs. This outbreak in a breeding unit was characterized by mastitis, suppurative metritis, and abortion in the sows, and acute enteritis and death from septicaemia in the piglets. By means of vaccine, penicillin therapy, surgical intervention where nec...

Katz, J.L., 1971: Elastic properties of calcified tissues. Israel Journal of Medical Sciences 7(3): 439-441

Robinson, R.C.; Holm, R.L., 2010: Orthognathic surgery for patients with maxillofacial deformities. Orthognathic surgery is performed to alter the shape of the jaws to improve dental occlusion stability, improve temporomandibular joint function, open the oropharyngeal airway, and improve the patient's facial proportions. Surgery must be coo...

Eriksson, N.E.; Holmen, A., 1996: Skin prick testing with inhalant allergens in eight countries. Allergy (Copenhagen) 51(SUPPL 32): 58

Shaidullin, I.N.; Zhivalev, I.K.; Shikhov, I.Y., 1994: Cytogenetic characteristics of hybrids of Ovis nivicola nivicola. Karyotyping of bone marrow cells was carried out on 1 snow sheep (Ovis nivicola nivicola) [male], 5 [female] and 3 [male] domestic sheep, and 3 [female] and 2 [male] hybrids between the 2 species. The diploid chromosome number was 52 in the snow s...