+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cholix-NAD+ Structure and Kinetic Analysis

, : Cholix-NAD+ Structure and Kinetic Analysis.

Certain Vibrio cholerae strains produce cholix, a potent protein toxin that has diphthamide-specific ADP-ribosyltransferase activity against eukaryotic elongation factor 2. Here we present a 1.8 crystal structure of cholix in complex with its natural substrate, nicotinamide adenine dinucleotide (NAD+). We also substituted hallmark catalytic residues by site-directed mutagenesis and analyzed both NAD+ binding and ADP-ribosyltransferase activity using a fluorescence-based assay. These data are the basis for a new kinetic model of cholix toxin activity. Further, the new structural data serve as a reference for continuing inhibitor development for this toxin class.


Other references

Khan, R.J.K.; Fick, D.; Khoo, P.; Yao, F.; Nivbrant, B.; Wood, D., 2006: Less invasive total hip arthroplasty: description of a new technique. We describe a new technique and aim to justify its use in total hip arthroplasty. The incision is short and there is minimal soft tissue dissection: piriformis and most of quadratus femoris remain intact. A meticulous capsular repair is performed....

Church M.K.; Polosa R.; Rimmer S.J., 1991: Cromolyn sodium and nedocromil sodium mast cell stabilizers neuromodulators or anti inflammatory drugs. Kaliner, M A , P J Barnes And C G A Persson (Ed ) Lung Biology in Health And Disease, Vol 49 Asthma: Its Pathology And Treatment Xxi+779p Marcel Dekker, Inc : New York, New York, Usa; Basel, Switzerland Illus 561-594

Verhoef, J.; Kerr, A., 2009: Delineating Canadas continental shelf in the Atlantic and Arctic oceans. Atlantic Geology 45: 93

Gambolati Giuseppe; Ricceri G.; Bertoni W.; Brighenti G.; Vuillermin E., 1991: Numerical analysis of land subsidence at Ravenna due to water withdrawal and gas removal. IAHS-AISH Publication 200: 119-128

Franzetti, L.; Galli, A.; Riva, M., 1996: Study of growth and fermentation activity of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus, using classic methods and calorimetry. The behaviour of 2 strains of S. thermophilus and L. delbrueckii subsp. bulgaricus isolated from commercial yoghurt was studied in reconstituted sterile low-fat milk at 38, 42 and 45 degrees C. Classic techniques involving plate counts and determi...

Flick, Ce, 1983: Isolation of mutants from cell culture. Handbook of plant cell culture(1): 393-441

Miyata, M.; Matsuda, Y.; Nomoto, M.; Takamatsu, Y.; Sato, N.; Hamatsu, M.; Dawson, P.A.; Gonzalez, F.J.; Yamazoe, Y., 2008: Cholesterol feeding prevents hepatic accumulation of bile acids in cholic acid-fed farnesoid X receptor (FXR)-null mice: FXR-independent suppression of intestinal bile acid absorption. Cholic acid (CA) feeding of farnesoid X receptor (Fxr)-null mice results in markedly elevated hepatic bile acid levels and liver injury. In contrast, Fxr-null mice fed cholesterol plus CA (CA+Chol) do not exhibit liver injury, and hepatic bile aci...

Holland, Heinrich D., 2008: Redox states during the Hadean. Astrobiology 8.5

Akkoyunlu, M.Emin.; Kart, L.; Uludağ, M.; Bayram, M.; Alisha, G.; Özçelik, H.; Karaköse, F.; Sezer, M., 2013: Relationship between symptoms of obstructive sleep apnea syndrome and traffic accidents in the city drivers. There is a high tendency for traffic accidents in patients with obstructive sleep apnea syndrome (OSAS). Thus it's recommended to investigate OSAS symptoms before certification of professional drivers. However, to what degree OSAS symptoms pr...

Vollmer Filho, G.; Bernardi, R., 1996: The multiple functions of the supervisor: a summary of the seventh IPA conference of training analysis. International Journal of Psycho-Analysis 77: 813-818