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Kv15-Kvbeta13 and PKC


, : Kv15-Kvbeta13 and PKC.

Kv1.5 channels are the primary channels contributing to the ultrarapid outward potassium current (IKur). The regulatory Kv?1.3 subunit converts Kv1.5 channels from delayed rectifiers with a modest degree of slow inactivation to channels with both fast and slow inactivation components. Previous studies have shown that inhibition of PKC with calphostin C abolishes the fast inactivation induced by Kv?1.3. In this study, we investigated the mechanisms underlying this phenomenon using electrophysiological, biochemical, and confocal microscopy approaches. To achieve this, we used HEK293 cells (which lack Kv? subunits) transiently cotransfected with Kv1.5+Kv?1.3 and also rat ventricular and atrial tissue to study native ?-? subunit interactions. Immunocytochemistry assays demonstrated that these channel subunits colocalize in control conditions and after calphostin C treatment. Moreover, coimmunoprecipitation studies showed that Kv1.5 and Kv?1.3 remain associated after PKC inhibition. After knocking down all PKC isoforms by siRNA or inhibiting PKC with calphostin C, Kv?1.3-induced fast inactivation at +6 mV was abolished. However, depolarization to +1 mV revealed Kv?1.3-induced inactivation, indicating that PKC inhibition causes a dramatic positive shift of the inactivation curve. Our results demonstrate that calphostin C-mediated abolishment of fast inactivation is not due to the dissociation of Kv1.5 and Kv?1.3. Finally, immunoprecipitation and immunocytochemistry experiments revealed an association between Kv1.5, Kv?1.3, the receptor for activated C kinase (RACK1), PKC?I, PKC?II, and PKC? in HEK293 cells. A very similar Kv1.5 channelosome was found in rat ventricular tissue but not in atrial tissue.

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Other references

Motin, V.G.; Iasnetsov, V.V.; Zabozlaev, A.A.; Karsanova, S.K.; Iasnetsov, V.V., 2012: Electrophysiological study of the mechanism of mexidol action. It has been found that mexidol (5 mM) significantly (96 +/- 2%) depressed excitatory postsynaptic current caused by step depolarization in neurons of medial vestibular nucleus of medulla oblongata slices in young (aged 13 - 17 days) male albino ra...

Shibaeva, A.N., 1972: Hygiene of sexual life. Fel'dsher i Akusherka 37(3): 41-43

Walker R.A., 1982: The use of the lectin from lotus tetragonolobus in the study of human breast tissue. Journal of Pathology 138(1): 79

Tammaro, F., 1983: New or rare Carex for the flora of Abruzzo and central southern Italy. Archivio botanico e biogeografico italiano9(3-4): 175-178

Y.Y.anChun; Zhao YongYan; Zeng ShuCai, 1998: Status in soils under different forest stands on the hilly regions of southern Jiangsu Province. Dynamic properties of nutrient status in soils under plantations of Chinese fir (Cunninghamia lanceolata), loblolly pine (Pinus taeda) and Phyllostachys pubescens, and a secondary oak (Quercus variabilis) plantation at the same age (25-years-old),...

Cocchi, R.; Fusari, A.; Favuto, F.; Lorini, G., 1978: Acupuncture, "vital" drugs and psychotropic agents in psychiatric patients. 50 cases. A therapeutical trial by means of acupuncture and "vital" drugs (1-glutamine and N-dipropylacetic acid) in 23 out of 50 psychiatric chronic patients basically depressive, and by means of acupuncture, vital drugs and psychodrugs in the re...

Lambeth, C.C.; Dvorak, W.S.; Endo, M., 1990: White pine plantings in the Highlands of Colombia. Notes are given on the nursery management of Pinus chiapensis, the performance of the species in comparison with other white pines, the performance of different provenances and wood properties in 15 trials and pilot plantations for pulpwood produc...

Desjardins A.E.; Spencer G.F.; Plattner R.D., 1989: Tolerance and metabolism of furanocoumarins by the phytopathogenic fungus gibberella pulicaris fusarium sambucinum. Sixty-two strains of Gibberella pulicaris (anamorph: Fusarium sambucinum) from diseased plants [Pastinaca sativa] and from soil were tested for tolerance of the furanocourmarin xanthotoxin in vitro. Twenty-one (88%) of the plant-derived strains an...

McKeown, P.P.; Gutterman, D., 2005: Executive summary: American College of Chest Physicians guidelines for the prevention and management of postoperative atrial fibrillation after cardiac surgery. Chest 128(2 Suppl): 1s-5s

Huo, L.; Guo, X.; Li, Y.; Hou, J., 2011: Synthesis of a polythieno[3,4-b]thiophene derivative with a low-lying HOMO level and its application in polymer solar cells. A planar benzodithiophene with lower HOMO was copolymerized with the thieno[3,4-b]thiophene unit to obtain a new low band gap polymer of PBDPTT-C, which exhibited a higher open-circuit voltage (V(oc)) of 0.8 V and a promising efficiency of 5.2%.