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FBLP-1 in Bone Remodeling

, : FBLP-1 in Bone Remodeling.

Bone remodeling is a complex process that must be precisely controlled to maintain a healthy life. We show here that filamin-binding LIM protein 1 (FBLP-1, also known as migfilin), a kindlin- and filamin-binding focal adhesion protein, is essential for proper control of bone remodeling. Genetic inactivation of FBLIM1 (the gene encoding FBLP-1) in mice resulted in a severe osteopenic phenotype. Primary FBLP-1 null bone marrow stromal cells (BMSCs) exhibited significantly reduced extracellular matrix adhesion and migration compared with wild type BMSCs. Loss of FBLP-1 significantly impaired the growth and survival of BMSCs in vitro and decreased the number of osteoblast (OB) progenitors in bone marrow and OB differentiation in vivo. Furthermore, the loss of FBLP-1 caused a dramatic increase of osteoclast (OCL) differentiation in vivo. The level of receptor activator of nuclear factor ?B ligand (RANKL), a key regulator of OCL differentiation, was markedly increased in FBLP-1 null BMSCs. The capacity of FBLP-1 null bone marrow monocytes (BMMs) to differentiate into multinucleated OCLs in response to exogenously supplied RANKL, however, was not different from that of WT BMMs. Finally, we show that a loss of FBLP-1 promotes activating phosphorylation of ERK1/2. Inhibition of ERK1/2 activation substantially suppressed the increase of RANKL induced by the loss of FBLP-Our results identify FBLP-1 as a key regulator of bone homeostasis and suggest that FBLP-1 functions in this process through modulating both the intrinsic properties of OB/BMSCs (i.e., BMSC-extracellular matrix adhesion and migration, cell growth, survival, and differentiation) and the communication between OB/BMSCs and BMMs (i.e., RANKL expression) that controls osteoclastogenesis.


Other references

De-Grandpre, L.B.rgeron, Y., 1997: Diversity and stability of understory communities following disturbance in the southern boreal forest. We investigated the response of boreal understorey communities to gap formation in order to evaluate the relationship between community stability and changes in community composition. We described the early changes in understorey species compositi...

Manevich, V.L.; Bogin, I.N.; Pogosian, A.M., 1980: Thermography in the overall diagnosis of the early stages of thyroid cancer. Meditsinskaia Tekhnika: 56-58

Lebedeva, T.V.; Sasu, S.; Singh, A.K., 1997: Regulation of the human IL-1beta promoter differs from that of the murine promoter. Journal of the American Society of Nephrology 9(PROGRAM AND ABSTR ISSUE): 460A, Sept

Zhou, H.; Yu, F.; Sun, J.; He, R.; Chen, S.; Chu, C-Wu.; Ren, Z., 2017: Highly active catalyst derived from a 3D foam of Fe(PO 3 ) 2 /Ni 2 P for extremely efficient water oxidation. Commercial hydrogen production by electrocatalytic water splitting will benefit from the realization of more efficient and less expensive catalysts compared with noble metal catalysts, especially for the oxygen evolution reaction, which requires a...

Krawany, H., 1937: Triohopterenstudien. Internationale Revue der Hydrobiologie Leipzig, 35: 318-327

Saranadasa, H., 2000: RSD requirement for different sample size for blend sampling. A formula for calculating the equivalent relative standard deviation (RSD) for different sample sizes other than the Office of Generic Drugs (OGD) draft guidance was derived. These critical values vary with the population RSD and the sample size a...

Silveira, Fábio.Porto.; Nicoluzzi, Jão.Eduardo.; Saucedo Júnior, N.Saucedo.; Silveira, Fábio.; Nicollelli, G.Matiolli.; Maranhão, B.Souza.De.Albuquerque., 2012: Evaluation of serum levels of interleukin-6 and interleukin-10 in patients undergoing laparoscopic versus conventional cholecystectomy. To correlate serum preoperative and postoperative interleukin-6 and interleukin-10 levels in patients undergoing laparotomy versus laparoscopic cholecystectomy. From a total of 20 patients, 18 were included in the study, nine underwent cholecystec...

Harrison J.G.; Scott R.E.; Urquhart L.A.; Simpson T., 1968: Hazards to birds from wool and other fibers vanellus vanellus foot injury larus canus entanglement. British Birds 61(10): 466-467

Dioli, Paride., 1993: Quasi due secoli di entomologia in Valtellina e Valchiavenna. Museologia Scientifica. Gennaio-Giugno; 101-2: 143-152

Vanmoor, A., 1998: Safe sex assurance devices. Official Gazette of the United States Patent & Trademark Office Patents 1217(2): 1213, Dec 8