geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

FAK and PKG Cooperate in Osteoblast Mechanotransduction


, : FAK and PKG Cooperate in Osteoblast Mechanotransduction.

Mechanical loading of bone induces interstitial fluid flow, leading to fluid shear stress (FSS) of osteoblasts. FSS rapidly increases the intracellular calcium concentration ([Ca2+]) and nitric oxide (NO) synthesis in osteoblasts and activates the protein kinase Akt. Activated Akt stimulates osteoblast proliferation and survival, but the mechanism(s) leading to Akt activation is not well defined. Using pharmacological and genetic approaches in primary human and mouse osteoblasts and mouse MC3T3 osteoblast-like cells, we found that Akt activation by FSS occurred through two parallel pathways; one required calcium stimulation of NO synthase and NO/cGMP/protein kinase G II-dependent activation of Src, and the other required calcium activation of FAK and Src, independent of NO. Both pathways cooperated to increase PI3K-dependent Akt phosphorylation and were necessary for FSS to induce nuclear translocation of ?-catenin, c-fos, and cox-2 gene expression and osteoblast proliferation. These data explain how mechanical stimulation of osteoblasts leads to increased signaling through a growth regulatory pathway essential for maintaining skeletal integrity.

US$29.90


Other references

Kurth, H.; Rossberg, D.; Koppen, H., 1984: Ein Simulationsmodell der Populationsdynamik des Kartoffelkafers, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Konzeption und okologische Grundlagen von SIMLEP. Population development of the Colorado beetle is simulated by SIMLEP from spring emergence of adults until the end of Sept. The population was subdivided into compartments: adults, eggs, larvae (1-2 instar), larvae (3-4 instar), pupae. Ecological...

Chen J.S.; Barbetti M.; Menesini Chen M.G.; Sensini C.; Baccetti B., 1991: Benzamidine resistant protease in acid extracted bull epididymal spermatozoa. Baccetti, B (Ed ) Serono Symposia Publications From Raven Press, Vol 75 Comparative Spermatology 20 Years After; Papers Presented at The 6th International Congress Of Spermatology, Siena, Italy, August 30-September 5, 1990 Xxxvi+1112p Raven Press: New York, New York, Usa Illus 125-128

Sato, M.; Bagchi, D.; Tosaki, A.; Das, D.K., 2001: Grape seed proanthocyanidin reduces cardiomyocyte apoptosis by inhibiting ischemia/reperfusion-induced activation of JNK-1 and c-Jun. The mechanism of cardioprotection with red wine consumption was studied by examining the antideath signaling cascade of one of the principle components of red wine, proanthocyanidins. Grape seed proanthocyanidin extract (GSPE) was administered ora...

Davila, E.; Velez, M.G.; Heppelmann, C.J.; Celis, E., 2002: Creating space: an antigen-independent, CpG-induced peripheral expansion of naive and memory T lymphocytes in a full T-cell compartment. Many of the mechanisms that govern T-cell homeostasis remain obscure. Here we report that repeated administration of synthetic oligodeoxynucleotides containing unmethylated cytosine-guanine motifs (CpG-ODN) into mice induces a systemic antigen-ind...

Schousboe, A.; Westergaard, N., 1993: Pathologic consequences in hippocampus of aberrations in the metabolic trafficking between neurons and glial cells necessary for normal glutamate homeostasis. Hippocampus 3 Spec No: 165-170

Liu DongLan; Zheng XiaoXian, 1999: Studies on artificial multiple storied stands. International research on artificial (planted) multistoried forest stands is reviewed. Aspects discussed include the light environment, growth models and management assessment. Such stands are a means of achieving sustainable plantation management...

Glasgow, L.A.; Crane, J.L.; Kern, E.R., 1978: Antitumor activity of interferon against murine osteogenic sarcoma cells in vitro. Murine interferon inhibited the growth of a continuous line of osteogenic sarcoma (OGS) cells in tissue culture. Inhibition of tumor cell growth by interferon was demonstrated by: a) decreased colony formation in soft agar, b) suppression of clone...

Kerkhof, G.A.; Waterman, D.; Lange, F., 2000: Circadian phase of chronic insomniacs and their sleep characteristics. Journal of Sleep Research 9(Supplement 1): 99, September

Anon,, 1967: Henry Higgins Lane (1878-1965) Biographical data. Misc Mus. nat. Hist. Univ. Kans: 1-8

Okada, Y.; Osada, M.; Kurata, S.I.hi; Sato, S.; Aisaki, K.I.hi; Kageyama, Y.; Kihara, K.; Ikawa, Y.; Katoh, I., 2002: P53 gene family p51 -encoded, secondary transactivator p51B occurs without forming an immunoprecipitable complex with MDM2, but responds to genotoxic stress by accumulation. p51(p63), a member of the p53 tumor suppressor gene family, generates multiple isoforms, including the potent and less potent transactivators p51A(TAp63gamma) and p51B(TAp63alpha), respectively, the latter poorly characterized for its protein feat...