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Tracing the conversion process from primordial germ cells to pluripotent stem cells in mice


, : Tracing the conversion process from primordial germ cells to pluripotent stem cells in mice. Biology of Reproduction 86(6): 182-182

To understand mechanisms underlying acquisition of pluripotency, it is critical to identify cells that can be converted to pluripotent stem cells. For this purpose, we focused on unipotent primordial germ cells (PGCs), which can be reprogrammed into pluripotent embryonic germ (EG) cells under defined conditions. Treatment of PGCs with combinations of signaling inhibitors, including inhibitors of MAP2K (MEK), GSK3B (GSK-3beta), and TGFB (TGFbeta) type 1 receptors, induced cells to enter a pluripotent state at a high frequency (12.1%) by Day 10 of culture. When we employed fluorescence-activated cell sorting to monitor conversion of candidate cells to a pluripotent state, we observed a cell cycle shift to S phase, indicating enrichment of pluripotent cells, during the early phase of EG formation. Transcriptome analysis revealed that PGCs retained expression of some pluripotent stem cell-associated genes, such as Pou5f1 and Sox2, during EG cell formation. On the other hand, PGCs lost their germ lineage characteristics and acquired expression of pluripotent stem cell markers, such as Klf4 and Eras. The overall gene expression profiles revealed by this system provide novel insight into how pluripotency is acquired in germ-committed cells.

US$19.90

PMID: 22423052

DOI: 10.1095/biolreprod.111.096792


Other references

Rice, JO.; Wunder, CC.; Diecke, FPJ.; Clark, MM.; Kesterson, RK.; White, JG.; Whiteside, KM., 1972: Chronic centrifugation of labyrinthectomized growing turtles. Proceedings of the Iowa Academy of Science, 78(3-4): 65-66

Juranic, A., 1933: Titration of Bacteria in Milk by Microscopic and Cultural Methods. For the hygienic control of milk and milk products it is of assistance to use the microscopic bacterial count. This method is less expensive and quicker than the cultural method. The number of bacteria found by the microscopic method is always hig...

Oh, J.Han.; Oh, C.Hee.; Choi, J-Ah.; Kim, S.Hoon.; Kim, J.Hyuk.; Yoon, J.Pil., 2012: Comparison of glenohumeral and subacromial steroid injection in primary frozen shoulder: a prospective, randomized short-term comparison study. (GH) joint steroid injection is one of the most well-known treatments for frozen shoulder. However, the low accuracy of GH joint injections and the improvement of symptoms after subacromial (SA) steroid injections led us to design a study that com...

Barnett, C.P.; Mendoza-Londono, R.; Blaser, S.; Gillis, J.; Dupuis, L.; Levin, A.V.; Chiang, P.W.; Spector, E.; Reardon, W., 2009: Aplasia of cochlear nerves and olfactory bulbs in association with SOX10 mutation. A 17-month-old boy was referred with profound sensorineural hearing loss (SNHL), severe visual impairment and developmental delay. Neuroimaging identified hypomyelination and cochlear nerve aplasia. He was noted to have fair skin and hair and mult...

Reuveni, R.; Madar, Z., 1985: The role of Macrophomina phaseolina in mortality of pine seedlings in forest nurseries. Pine seedlings in 3 forest nurseries in Israel were affected during the summer by a disease causing needle yellowing, wilting and death. M. phaseolina was isolated from roots of 5 Pinus spp. and its incidence correlated with seedling death. Fusari...

Horgen, Pa, 1981: Purification and characterization of RNA polymerase II from the aquatic fungus, Achiya ambisexualis. Experimental mycology 5(3): 193-208

Oliveira, O.L.; Aguiar, A.C.; D.C.rvalho, A.D., 1974: Distance between the lateral contour of the infra-orbital foramina in human skulls of adult Brazilian Whites, Negroes and Mulattoes. Arquivos do Centro de Estudos Da Faculdade de Odontologia Da U. F. M. G 11(2): 153-162

Mundt, T.; Heinemann, F.; Schankath, C.; Schwahn, C.; Biffar, R., 2014: Retrospective and clinical evaluation of retrievable, tooth-implant supported zirconia-ceramic restorations. Permanent cementations of zirconia-ceramic restorations may conflict with the rationale for retrievability of implant-supported restorations. The aim of this study was to test the hypothesis that retrievable, tooth-implant supported FDPs made of v...

Wadwa, K.S.; Venbrux, A.; Osterman, F.; Mitchell, S.; Punjabi, N.; Thuluvath, P.J., 1997: Transjugular intrahepatic portosystemic shunt may cause porto-pulmonary hypertension. Gastroenterology 112(4 SUPPL ): A1410

Carrico, R.M., 2011: Silent menace. C. difficile and its threat to health care facilities. Health Facilities Management 24(6): 43-45