geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evidence for a cytoplasmic microprocessor of pri-miRNAs


, : Evidence for a cytoplasmic microprocessor of pri-miRNAs. Rna 18(7): 1338-1346

MicroRNAs (miRNAs) represent a class of noncoding RNAs that fine-tune gene expression through post-transcriptional silencing. While miRNA biogenesis occurs in a stepwise fashion, initiated by the nuclear microprocessor, rare noncanonical miRNAs have also been identified. Here we characterize the molecular components and unique attributes associated with the processing of virus-derived cytoplasmic primary miRNAs (c-pri-miRNAs). RNA in situ hybridization and inhibition of cellular division demonstrated a complete lack of nuclear involvement in c-pri-miRNA cleavage while genetic studies revealed that maturation still relied on the canonical nuclear RNase III enzyme, Drosha. The involvement of Drosha was mediated by a dramatic relocalization to the cytoplasm following virus infection. Deep sequencing analyses revealed that the cytoplasmic localization of Drosha does not impact the endogenous miRNA landscape during infection, despite allowing for robust synthesis of virus-derived miRNAs in the cytoplasm. Taken together, this research describes a unique function for Drosha in the processing of highly structured cytoplasmic RNAs in the context of virus infection.

US$19.90

PMID: 22635403

DOI: 10.1261/rna.032268.112


Other references

Han, G.Cheol.; Ko, S.Kwon.; Sung, J.Hwan.; Chung, S.Hyun., 2007: Compound K enhances insulin secretion with beneficial metabolic effects in db/db mice. Compound K (CK) is a final metabolite of panaxadiol ginsenosides. Although Panax ginseng is known to have antidiabetic activity, the active ingredient is not yet fully identified. In our preliminary studies, panaxadiol ginsenosides showed insulin...

Behnke, B.J.; Stabley, J.N.; McCullough, D.J.; Davis, R.T.; Dominguez, J.M.; Muller-Delp, J.M.; Delp, M.D., 2013: Effects of spaceflight and ground recovery on mesenteric artery and vein constrictor properties in mice. Following exposure to microgravity, there is a reduced ability of astronauts to augment peripheral vascular resistance, often resulting in orthostatic hypotension. The purpose of this study was to test the hypothesis that mesenteric arteries and v...

Anonymous, 2012: Tumour-seeding a rare complication of ablative therapy for clinically localised renal cell carcinoma. Current evidence-based clinical practice guidelines identify surgical resection as the recommended treatment of small renal masses. Ablative approaches such as laparoscopic and percutaneous cryoablation and radiofrequency ablation offer the promis...

Yamada, N., 1983: Oral manifestations of systemic genetic diseases. Shikai Tenbo 62(1): 71-78

Glock, Y.; Benallou, A.; Puel, P., 1984: Extra-anatomical shunts in the lower limbs. Apropos of a series of 51 consecutive cases. Indications for extra-anatomical shunts are defined on the basis of results of 51 consecutive operations. Axillo-uni or bi-femoral (AF) shunts (36 cases) and femoro-femoral (FF) shunts (15 cases) were performed in elderly men (74% over 60 years of...

Stager S.V.; Freeman F.J., 1992: Representing laryngeal reaction time performance. Different calculation methods have been used to derive laryngeal reaction time (LRT) values. Some represent average LRT performance and others, optimal. Whether experimental outcome differed depending on choice of calculation method was tested. Ou...

Wachendorf H.; Baumann A.; Gwosdz W.; Schneider W., 1974: The phyllite series of eastern Crete; a melange. Zeitschrift der Deutschen Geologischen Gesellschaft 125(2): 237-251

MacKinnon, B.; Laesser, J.; Rotenberg, J.; Tellez, L.I., 2003: Eremophila alpestris (Alaudidae): A new bird species and family for the Yucatan Peninsula, Mexico. This note reports on the first record of horned lark on November 23, 2002 at Progreso, on the north coast of Mexico's Yucatan Peninsula. The closest migratory population reaches the Texas coast and conceivably this individual crossed the Gulf...

Amon, F., 1995: Legal liability in clinical trials from the legal-pharmaceutical viewpoint. In this paper, the different liability rules for clinical trials are demonstrated and the contractual liability and the liability law for products are explained. The protection of the study participants by an insurance is demonstrated by an exampl...

Ornstein, K.A.; Penrod, J.; Schnur, J.B.; Smith, C.B.; Teresi, J.A.; Garrido, M.M.; McKendrick, K.; Siu, A.L.; Meier, D.E.; Morrison, R.Sean., 2017: The Use of a Brief 5-Item Measure of Family Satisfaction as a Critical Quality Indicator in Advanced Cancer Care: A Multisite Comparison. Although family satisfaction is recognized as a critical indicator of quality for patients with advanced cancer, it is rarely assessed as part of routine clinical care. Measurement burden may be one barrier to widespread use of family satisfaction...