geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Monocytic AML cells inactivate antileukemic lymphocytes: role of NADPH oxidase/gp91(phox) expression and the PARP-1/PAR pathway of apoptosis


, : Monocytic AML cells inactivate antileukemic lymphocytes: role of NADPH oxidase/gp91(phox) expression and the PARP-1/PAR pathway of apoptosis. Blood 119(24): 5832-5837

Dysfunction of T cells and natural killer (NK) cells has been proposed to determine the course of disease in acute myeloid leukemia (AML), but only limited information is available on the mechanisms of lymphocyte inhibition. We aimed to evaluate to what extent human malignant AML cells use NADPH oxidase-derived reactive oxygen species (ROS) as an immune evasion strategy. We report that a subset of malignant myelomonocytic and monocytic AML cells (French-American-British [FAB] classes M4 and M5, respectively), recovered from blood or BM of untreated AML patients at diagnosis, expressed the NADPH oxidase component gp91phox. Highly purified FAB M4/M5 AML cells produced large amounts of ROS on activation and triggered poly-[ADP-ribose] polymerase-1–dependent apoptosis in adjacent NK cells, CD4+ T cells, and CD8+ T cells. In contrast, immature (FAB class M1) and myeloblastic (FAB class M2) AML cells rarely expressed gp91phox, did not produce ROS, and did not trigger NK or T-cell apoptosis. Microarray data from 27 AML patients confirmed a greater expression of gp91phox mRNA by FAB-M4/M5 AML cells than FAB-M1 cells (P < 1–11) or FAB-M2 cells (P < 1–9). Our data are suggestive of a novel mechanism by which monocytic AML cells evade cell-mediated immunity.

US$19.90

PMID: 22550344

DOI: 10.1182/blood-2011-11-391722


Other references

Forbes, G., 1947: The effect of alcohol on psychomotor reactions as a possible index of the degree of alcoholic intoxication. Medico-Legal and Criminological Review 15(1): 23-38

Poradovský; Schwarz, T., 1967: 75th anniversary of professor MUDr. Teodor Schwarz. Ceskoslovenska Gynekologie 32(10): 774-774

Freiherr von Salis-Soglio, G.; Uhlig, T., 1988: Injuries of the biceps brachii muscle with special reference to surgical therapy. Injuries of M. biceps brachii are rare, in most cases (greater than 90%) the long tendon is involved, seldom the distal tendon. The long tendon usually ruptures in the sulcus intertubercularis, preexisting degenerative changes play an important ro...

Raatiniemi, L.; Mikkelsen, K.; Fredriksen, K.; Wisborg, T., 2014: Do pre-hospital anaesthesiologists reliably predict mortality using the NACA severity score? A retrospective cohort study. The National Advisory Committee on Aeronautics' (NACA) severity score is widely used in pre-hospital emergency medicine to grade the severity of illness or trauma in patient groups but is scarcely validated. The aim of this study was to asses...

Stamenkovic, T.K.stic, M.M.lanovic, S.M.lanovic, M., 1981: Changes of young beef and pork meat during heat processing. Hrana i ishrana = Food and nutrition 22(1-2): 13-15

Allison, R.S., 1932: The Fractional Test-Meal as a Guide to Delay in Emptying of the Stomach. Ulster Medical Journal 1(3): 176-182

Kutzner, J.; Sachs, A.; Grimm, W., 1978: A comparative radioisotope study of calcium metabolism in bone and teeth of rats. Investigations with bone-seeking radionuclides (47Ca, 99mTc-PP, 113Sn) in rats of different age demonstrated a true accumulation in teeth as compared to bone. Blood flow was evaluated by 99mTcO4 and 51Cr-Albumin. Differences of accumulation depend...

Ketchel, M.M.; Sturgis, S.H., 1959: Influence of prochlorperazine on hydrocortisone inhibition of in vitro leucocyte migration. Addition of hydrocortisone to human blood inhibited ameboid migration of leucocytes in capillary tubes. When a patient was treated with prochlorperazine, however, this inhibition did not occur. This observation led to the present work wherein vary...

Whitbeck, L.B.; Yu, M.; Johnson, K.D.; Hoyt, D.R.; Walls, M.L., 2008: Diagnostic prevalence rates from early to mid-adolescence among indigenous adolescents: first results from a longitudinal study. Investigate change in prevalence rates for mental and substance abuse disorders between early and mid-adolescence among a cohort of indigenous adolescents. The data are from a lagged, sequential study of 651 indigenous adolescents from a single cu...

Lediaeva, E.M., 1954: Stimulation of mitotic divisions in the hepatic cells. Arkhiv Anatomii, Gistologii i Embriologii 31(2): 24-26