+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Validation of the rapid fluorescent focus inhibition test for rabies virus-neutralizing antibodies in clinical samples

, : Validation of the rapid fluorescent focus inhibition test for rabies virus-neutralizing antibodies in clinical samples. Antimicrobial Agents and ChemoTherapy 56(7): 3524-3530

Monoclonal antibodies are successful biologics in treating a variety of diseases, including the prevention or treatment of viral infections. CL184 is a 1:1 combination of two human monoclonal IgG1 antibodies (CR57 and CR4098) against rabies virus, produced in the PER.C6 human cell line. The two antibodies are developed as replacements of human rabies immune globulin (HRIG) and equine rabies immune globulin (ERIG) in postexposure prophylaxis (PEP). The rapid fluorescent focus inhibition test (RFFIT) is a cell-based virus neutralization assay which is usually performed to determine the biological potency of a vaccine and to measure the levels of protection against rabies in humans and animals. In order to confirm the suitability of this assay as a pharmacodynamic assay, we conducted a validation using both HRIG- and CL184-spiked serum samples and sera from vaccinated donors. The validation results met all analytical acceptance criteria and showed that HRIG and CL184 serum concentrations can be compared. Stability experiments showed that serum samples were stable in various suboptimal conditions but that rabies virus should be handled swiftly once thawed. We concluded that the assay is suitable for the measurement of polyclonal and monoclonal rabies neutralizing antibodies in clinical serum samples.


PMID: 22547629

DOI: 10.1128/AAC.06179-11

Other references

Howard, F.W.; Verkade, S.D.; DeFilippis, J.V., 1990: Propagation of West Indies mahogany, Swietenia mahagoni, by cuttings. A 5-yr-old tree of S. mahagoni was severely pruned in March 1987 to simulate a spring growth flush; 28 cuttings 20 cm long and trimmed of all but five leaves were made in May 1987. Cuttings were of two types: 14 cut from the basal end of the curre...

Geisler, J.P.; Goulet, R.; Foster, R.S.; Sutton, G.P., 1994: Growing teratoma syndrome after chemotherapy for germ cell tumors of the ovary. Background: The growing teratoma syndrome has been described with regard to gonadal and extragonadal germ cell neoplasms in males, but few cases have been reported in the female population. In this condition, masses that enlarge during or after ch...

Luciani, N.; Nasso, G.; D'Alessandro, C.; Testa, F.; Glieca, F.; Gaudino, M.; Possati, G., 2002: Heart surgery interventions in chronic dialysis patients: short- and long-term results. Chronic dialysis is a relevant risk factor for mortality and morbidity after cardiac surgery and cardiopulmonary bypass. The aim of this study was to evaluate the short- and long-term follow-up of patients in dialysis undergoing cardiac surgery. W...

Yu, W.H.uan; Woessner, J.F.ederick; Mcnish, J.; Stamenkovic, I., 2002: A lesson from CD44HSPG null mice Heparan sulfate mediate CD44HSPG transactivating erbB4 pathway implicating the survival mechanism for epithelial/smooth muscle cells during the postpartum reproductive organism extra cellular matrix remodeling. Glycobiology 12(10): 652, October

Gomes, M.Cesarino.; Clementino, M.Alves.; Pinto-Sarmento,; Martins, C.Castro.; Granville-Garcia, A.Flávia.; Paiva, S.Martins., 2015: Association between parental guilt and oral health problems in preschool children: a hierarchical approach. Dental caries and traumatic dental injury (TDI) can play an important role in the emergence of parental guilt, since parents feel responsible for their child's health. The aim of the present study was to evaluate the influence of oral health...

Zauberman, A.; Flusberg, D.; Haupt, Y.; Barak, Y.; Oren, M., 1995: A functional p53-responsive intronic promoter is contained within the human mdm2 gene. The mdm2 gene is a target for transcriptional activation by the p53 tumor suppressor gene product. Previous work has revealed that the mouse mdm2 gene contains two promoters: one is located upstream to the gene and is active in the absence of p53,...

Gao, D-Yao.; Xu, Z-Shi.; He, Y.; Sun, Y-Wei.; Ma, Y-Zhi.; Xia, L-Qin., 2014: Functional analyses of an E3 ligase gene AIP2 from wheat in Arabidopsis revealed its roles in seed germination and pre-harvest sprouting. Pre-harvest sprouting (PHS) seriously affects wheat yield and quality of the grain. ABI3 is a key factor in the activation of seed development and repression of germination in Arabidopsis. An ABI3-interacting protein (AIP2) could polyubiquitinate...

Magendie, J.; Magimel, J.; Ponsan, X.; Dra, K., 1958: Menometrorrhagia in young girls; study of 30 cases. Bulletin de la Federation des Societes de Gynecologie et Dobstetrique de Langue Francaise 10(2): 188-188

Tandon, R.C.; Singh, J.B.; Dhyan Singh, 1993: Modified wirebound veneer boxes for horticulture produce. The advantages of wirebound boxes over conventional nailed wooden boxes and corrugated fibreboard boxes for use in India is discussed and details are given of the manufacture and tesing of wirebound boxes made from veneers of Abies pindrow. Data a...

Anonymous, 1973: Conflicting arrangements, conflicting expansion. Sygeplejersken 73(41): 3-3