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The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene


, : The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene. Antimicrobial Agents and ChemoTherapy 56(7): 3563-3567

The cfr gene encodes the Cfr methyltransferase that methylates a single adenine in the peptidyl transferase region of bacterial ribosomes. The methylation provides resistance to several classes of antibiotics that include drugs of clinical and veterinary importance. This paper describes a first step toward elucidating natural residences of the worrisome cfr gene and functionally similar genes. Three cfr-like genes from the order Bacillales were identified from BLAST searches and cloned into plasmids under the control of an inducible promoter. Expression of the genes was induced in Escherichia coli, and MICs for selected antibiotics indicate that the cfr-like genes confer resistance to PhLOPSa (phenicol, lincosamide, oxazolidinone, pleuromutilin, and streptogramin A) antibiotics in the same way as the cfr gene. In addition, modification at A2503 on 23S rRNA was confirmed by primer extension. Finally, expression of the Cfr-like proteins was verified by SDS gel electrophoresis of whole-cell extracts. The work shows that cfr-like genes exist in the environment and that Bacillales are natural residences of cfr-like genes.

US$19.90

PMID: 22547628

DOI: 10.1128/AAC.00673-12


Other references

Tsukamoto, K.; Sasaki, M.; Morita, F., 1952: Histochemical study of peroxidase. IX. Electron micrographs of blood cells with special reference to the crystals produced by the peroxidase reaction. Okajimas Folia Anatomica Japonica 24(3): 199-203

Pearce, I.G.; Chaplain, M.A.J.; Schofield, P.G.; Anderson, A.R.A.; Hubbard, S.F., 2006: Modelling the spatio-temporal dynamics of multi-species host-parasitoid interactions: heterogeneous patterns and ecological implications. A mathematical model of the spatio-temporal dynamics of a two host, two parasitoid system is presented. There is a coupling of the four species through parasitism of both hosts by one of the parasitoids. The model comprises a system of four reacti...

Antoniades, C.; Shirodaria, C.; Leeson, P.; Baarholm, O.A.; Van-Assche, T.; Cunnington, C.; Pillai, R.; Ratnatunga, C.; Tousoulis, D.; Stefanadis, C.; Refsum, H.; Channon, K.M., 2009: MTHFR 677 C>T Polymorphism reveals functional importance for 5-methyltetrahydrofolate, not homocysteine, in regulation of vascular redox state and endothelial function in human atherosclerosis. The role of circulating homocysteine as an atherosclerosis risk factor has recently been questioned. However, 5-methyl-tetrahydrofolate (5-MTHF), the circulating metabolite of folic acid participating in homocysteine metabolism, has direct effects...

Maeda, R.; Endou, K.; Suzuki, R.; Miyao, T.; Masushige, S.; Kida, S., 2003: Effects of forebrain-restricted overexpression of CaMKIV on learning and memory. Neuroscience Research Supplement (26): S49, 2002( )

Kim, E.Ji.; Oh, H.Young.; Heo, H-Sam.; Hong, J.Eun.; Jung, S-Jae.; Lee, K.Won.; Park, J.Hoon.; Hur, C-Goo.; Park, J.Han.Yoon., 2013: Biological features of core networks that result from a high-fat diet in hepatic and pulmonary tissues in mammary tumour-bearing, obesity-resistant mice. We previously demonstrated that the chronic consumption of a high-fat diet (HFD) promotes lung and liver metastases of 4T1 mammary carcinoma cells in obesity-resistant BALB/c mice. To examine early transcriptional responses to tumour progression i...

Lochhead, A.G., 1928: The Etiology Of European Foulbrood Of Bees. There is some evidence that Streptococcus apis and Bacillus pluton are actually morphological forms of Bacillus alvei. In the usual morphological forms Strep. apis and B. alvei are non-pathogenic, while B. pluton is usually stated to be the exciti...

Schover, L.R., 1997: Recognizing the stress of infertility. Although infertility is often blamed on stress, the evidence that psychological factors interfere with conception is slim. Far more salient is the stress that infertility imposes on patients and their marriages. Primary care physicians can help by...

Ellis, F.R.; Halsall, P.J.; Harriman, D.G., 1988: Malignant hyperpyrexia and sudden infant death syndrome. This paper reports three investigations of the suggested relationship between sudden infant death syndrome (SIDS) and malignant hyperpyrexia (MH). In the first study 151 MH-susceptible families completed a questionnaire designed to identify the in...

Chen, Y.L.; Ben, K.L.; Cao, X.M., 1996: The terminal monosaccharide of antigenic determinant is involved in the binding of human sperm to specific monoclonal IgA antibody. In order to know whether terminal monosaccharide of human sperm antigen is involved in stimulating antisperm immune response (especialy IgA production) in mucosal immune system, 33 IgA, 12 IgG and 35 IgM monoclonal antibodies (MAbs) all obtained b...

Mahon, F.X.; Marit, G.; Boiron, J.M.; Cony-Makhoul, P.; Agape, P.; Pigneux, A.; Broustet, A.; Reiffers, J., 1997: Autologous stem cell transplantation for chronic myeloid leukemia. Recent Results in Cancer Research. Fortschritte der Krebsforschung. Progres Dans les Recherches sur le Cancer 144: 8-14