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Adenosine-5'-triphosphate release by Mannheimia haemolytica, lipopolysaccharide, and interleukin-1 stimulated bovine pulmonary epithelial cells


, : Adenosine-5'-triphosphate release by Mannheimia haemolytica, lipopolysaccharide, and interleukin-1 stimulated bovine pulmonary epithelial cells. Veterinary Immunology and Immunopathology 149(1-2): 58-65

Mannheimia haemolytica, one of the agents associated with bovine respiratory disease complex, can cause severe lung pathology including the leakage of vascular products into the airways and alveoli. Previous work by this laboratory has demonstrated that bovine lung endothelial and epithelial cells undergo dramatic permeability increases when exposed to adenosine-5 -triphosphate (ATP). Therefore, we wanted to determine if ATP levels were elevated in bronchoalveolar lavage (BAL) samples from calves experimentally infected with M. haemolytica. In addition, cultured bovine pulmonary epithelial (BPE) cells were stimulated with heat-killed and live M. haemolytica bacteria, lipopolysaccharide (LPS), lipoteichoic acid (LTA), interleukin-1 (IL-1), and zymosan activated plasma (ZAP) to determine whether they might release extracellular ATP during in vitro infection. Calves experimentally exposed to M. haemolytica had an approximately 2-fold higher level of ATP in their BAL samples compared to control. BPE cells exposed to increasing numbers of heat-killed or live M. haemolytica had significantly increased levels of ATP release as compared to time-matched controls. Finally, BPE cells treated with several concentrations of LPS and IL-1 had increases in ATP release as compared to time-matched controls. This increase appeared to be a result of active ATP secretion by the cells, as cell viability was similar between treated and non-treated cells. Neither ZAP nor LTA induced any ATP release by the cells. In conclusion, ATP levels are elevated in lung secretions from calves infected with M. haemolytica. In addition, lung epithelial cells can actively release ATP when exposed to heat-killed or live M. haemolytica, LPS or IL-1.

US$19.90

PMID: 22771196

DOI: 10.1016/j.vetimm.2012.06.011


Other references

Ferrario, I.; Saputo, V.; Mancosu, M.; Colombo, B., 1970: New method of evaluation of the bacterial count agar culture (dip-slide) as a screening test for symptomatic and asymptomatic bacteriuria. Minerva Pediatrica 22(23): 1152-1156

Alhaiti, A.Hassan.; Alotaibi, A.Raffa.; Jones, L.Katherine.; DaCosta, C.; Lenon, G.Binh., 2017: Psychometric Evaluation of the Revised Michigan Diabetes Knowledge Test (V.2016) in Arabic: Translation and Validation. <i>Objective</i>. To translate the revised Michigan Diabetes Knowledge Test into the Arabic language and examine its psychometric properties. <i>Setting</i>. Of the 139 participants recruited through King Fahad Medical City...

Nosov, S.D.; Ladodo, K.S.; Soboleva, V.D.; Popova, L.A.; Kudashov, N.I., 1966: Clinical-pathogenetic features of influenza in children. Sovetskaia Meditsina 29(6): 50-55

Aiba, N.; Kondo, M., 2005: Dietary habits of elderly Japanese subjects for maintenance of immune function and improvement of QOL. To clarify the factors that suppress the increase in intracellular reactive oxygen species (ROS), the activities of 26 natural antioxidants were analysed. Results indicated that 13, including flavonoids, eliminated the intracellular ROS induced by...

Jeong, S-Jin.; Koh, W.; Kim, B.; Kim, S-Hoon., 2012: Are there new therapeutic options for treating lung cancer based on herbal medicines and their metabolites?. ETHONOPHARMACOLOGICAL RELEVANCE: Lung cancer is one of the most lethal cancers in terms of mortality and incidence worldwide. Despite intensive research and investigation, treatment of lung cancer is still unsatisfactory due to adverse effects and...

Pengo V.; Cartei G.; Casara D.; Guerra C.; Bertaglia E.; Zanella R.; Bajo M.; Belmonte P., 1981: Fibrinogen consumption in breast cancer and its relation to the extent and type of metastases. Thrombosis & Haemostasis 46(1): 360

Joseph, L., 2016: The clinical utility of molecular genetic cancer profiling. Next-Generation-Sequencing (NGS) has enabled gene mutation profiling - cataloguing sequence variants and modifications in clinical assays encompassing tens to thousands of genes in tumors and in germlines. The clinical benefit of applying multi-ge...

François, J.; D.L.ey, J.J., 1979: Classification of chorioretinal degenerations or dystrophies. Bulletin de la Societe Belge D'ophtalmologie 185: 47-54

Leonardi, G.; Sarjeant, WAS., 1986: Footprints representing a new Mesozoic fauna from Brazil. Modern Geology, 101: 73-84

Behrends, H.B.; Schomburg, E.D.; Steffens, H., 1978: Influence of spinal locomotion on segmental and intersegmental reflex pathways from cutaneous afferents to motoneurones [proceedings. Journal of Physiology 284: 175p-176p