+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Molecular cloning and expression analysis of interferon regulatory factor 8 (IRF8) in turbot, Scophthalmus maximus

, : Molecular cloning and expression analysis of interferon regulatory factor 8 (IRF8) in turbot, Scophthalmus maximus. Veterinary Immunology and Immunopathology 149(1-2): 143-150

Interferon regulatory factor 8 (IRF8) in mammals is known to be involved in antiviral response. In this study, the gene of IRF8 was cloned from the turbot (Scophthalmus maximus) fish and its expession in response to polyinosinic: polycytidylic acid (poly I:C) and turbot reddish body irrdovirus (TRBIV) challenges was studied. Turbot (Sm)IRF8 gene is 4363bp long, comprises nine exons and eight introns and encodes a putative 42 amino acid (aa) protein. The predicted protein sequence possesses a DNA binding domain (DBD), an IRF association domain (IAD) and a nuclear localization signal (NLS). Constitutive expression of SmIRF8 was detectable in all tested organs, with higher levels observed in the spleen, kidney and head kidney. SmIRF8 transcript levels were up-regulated by both poly I:C and TRBIV treatments in the spleen, head kidney, gills and muscle in an early phase of a 7-day time course and the poly I:C was a quicker inducer. In both challenge cases, the highest and earliest inductions were detected in the spleen, while the induction in the muscle was quite faint. These results provide insights into the role of SmIRF8 in antiviral response.


PMID: 22763146

DOI: 10.1016/j.vetimm.2012.06.008

Other references

Gothe, R.; Hartig, M., 1975: Acaricide-resistance of ixodide and argaside ticks. Dtw. Deutsche Tierarztliche Wochenschrift 82(10): 413-20 Contd

Noskov F.S.; Ermakov N.V.; Avdeenko M.M., 1968: Concentration and rapid detection of small pox vaccine virus on absorbents by the inst fluorescent antibody technique a technique for identifying this virus in a liquid medium rabbit anti vaccinia gamma globulin. Voprosy Virusologii 13(4): 494-498

Li, J.H.nriksson, G.G.llerstedt, G., 2007: Lignin depolymerization. Steam explosion is an important process for the fractionation of biomass components. In order to understand the behaviour of lignin under the conditions encountered in the steam explosion process, as well as in other types of steam treatment, aspe...

Silva, A.; Andersen, M.L.; Tufik, S., 2008: Sleep pattern in an experimental model of osteoarthritis. Osteoarthritis (OA) is a major healthcare burden of increasing prevalence. It has been demonstrated that the relationship between pain and sleep produces changes in sleep patterns and pain perception. However, electrophysiological studies in anima...

Smith, W.L., 2007: Nutritionally essential fatty acids and biologically indispensable cyclooxygenases. The study of cyclooxygenases (COXs), targets of aspirin and related drugs, is rooted in the discovery of essential fatty acids (EFAs). There are two COXs that convert EFAs, primarily arachidonic acid, to prostaglandins. Each COX is involved with d...

Parks, H.S.; Reinbold, G.W.; Hammond, E.G.; Clark, W.S., 1967: Growth of propionibacteria at low temperatures. Since bacterial growth in the cold room has been indicted as a cause of splits and checks in Swiss cheese, the growth of Propionibacterium spp. (33 stock cultures and 16 unclassified cultures freshly isolated from commercial Swiss cheese) was inve...

Anderson, M.C., 1993: Invasive carcinoma of the cervix following local destructive treatment for cervical intraepithelial neoplasia. Objective: To investigate the occurrence of invasive carcinomas following local destructive treatment for cervical intraepithelial neoplasia (CIN) and to evaluate the factors responsible. Design: A multicentre retrospective study. Subjects: Forty-...

Rudolph, M.; Destexhe, A., 2003: The discharge variability of neocortical neurons during high-conductance states. In vivo recordings have shown that the discharge of cortical neurons is often highly variable and can have statistics similar to a Poisson process with a coefficient of variation around unity. To investigate the determinants of this high variabili...

Goodwin, W.H., 2017: The use of forensic DNA analysis in humanitarian forensic action: The development of a set of international standards. DNA analysis was first applied to the identification of victims of armed conflicts and other situations of violence (ACOSV) in the mid-1990s, starting in South America and the Balkans. Argentina was the first country to establish a genetic databas...

Song Huichun.; Xiang Suliu.; Fan Yan., 1998: Fluorescence spectrum analysis of fresh water pipless pearl. Journal of Fishery Sciences of China. Dec; 54: 1-5