geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception


, : Predictive value of DNA integrity analysis in idiopathic recurrent pregnancy loss following spontaneous conception. Journal of Assisted Reproduction and Genetics 29(9): 861-867

Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception. Semen samples from 45 patients and 2 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI). By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL. Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.

US$19.90

PMID: 22692280

DOI: 10.1007/s10815-012-9801-3


Other references

Schober, O.; Lehr, L.; Hundeshagen, H., 1982: Bromide space, total body water, and sick cell syndrome. Displacements of the bromide space (Br-82-C, as a marker for the extracellular fluid compartment) are caused by an enhanced anatomical space and/or increased permeability of cells to bromide. The ratio Br-82-C: total body water (TBW) was evaluated...

Peruanskii, Y.V.; Sadykov, S.S., 1974: The composition of reserve proteins in the barley variety Hiproly. Electrophoretic analysis revealed that the globulin spectrum of Hiproly contained more components and the hordenin spectrum fewer components than Nutans 187. The hordein spectrum of Hiproly was more complex than that of Nutans 187, especially in t...

Mills, G.D.; Harris, D.M.; Chen, X.; Houser, S.R., 2006: Intracellular sodium determines frequency-dependent alterations in contractility in hypertrophied feline ventricular myocytes. Hypertrophy and failure (H/F) in humans and large mammals are characterized by a change from a positive developed force-frequency relationship (+FFR) in normal myocardium to a flattened or negative developed force-frequency relationship (-FFR) in...

Edwards, J.; Jobst, D.; Hodges, J.; Leyburn, M.; O'connor, L.K.; Macdonald, A.; Smith, G.F.; Wood, P., 1966: The Charolais Report. The results of field trials in England and Wales to compare Charolais bulls with bulls of British beef breeds when crossed with dairy cows. 2211, 2222, 2268, 2355, 2509, 2539, 2543, 2545, 2960, 3416, 3514, 3523, 3525, 3539, 3581, 3732, 3824, 3849, 3853, 3879, 3883.

Green, N.; Melbye, R.W.; Kernen, J., 1975: Stage i well differentiated adenocarcinoma of the endometrium. American Journal of Roentgenology, Radium Therapy, and Nuclear Medicine 123(3): 563-566

Valerianov Ts; Cholakova S.; Toschkoff Al, 1975: On the virulence of lysogenic strains erysipelothrix insidiosa. Acta Microbiologica Virologica et Immunologica 1: 66-69

Nencini, C.; Barberi, L.; Runci, F.M.; Micheli, L., 2008: Retinopathy induced by drugs and herbal medicines. Retina is the part of the eye suffering most damage from drugs. It is made up of a thin nervous membrane that covers the eye-ball internally, within the thickness of which three types of cells are ordered. In this paper we describe the drugs that...

Gupta, M.; Mahajan, J.K.; Bawa, M.; Rao, K.L.N., 2011: Esophageal atresia and tracheoesophageal fistula: Effect of pleural cover on anastomotic dehiscence. A significant number of esophageal atresia and tracheoesophageal fistula patients have long gaps and a high propensity to leak. Anastomotic leak in esophageal atresia is associated with a significant morbidity and mortality. In a prospective rando...

Chyczewski, L.; Debek, W., 1995: Endothelial cell activation in shock. Some pathological conditions including shock, induce focal activation, degeneration, necrosis and desquamation of endothelial cells. These changes cause disturbances of endothelial functions which include expression of adhesive molecules resulting...

Sataloff, J.; Wilson, J.F., 1951: Perforated ear drum following home permanent wave. Journal of the American Medical Association 147(12): 1135-1136