+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Genotoxic effects of tacrolimus on human lymphocyte cells

, : Genotoxic effects of tacrolimus on human lymphocyte cells. Genetika 48(6): 762-767

We designed in vitro study to determine possible genotoxic effects oftacrolimus (FK-506), which is used as a potent immunosuppressive drug, by using sister chromatid exchange (SCEs), chromosome aberration (CAs), micronuclei tests (MN) and cell growth kinetics such as mitotic index (MI) and replication index (RI) in human lymphocytes. The cells were treated with 5, 25, 50, and 100 ng/mL concentrations of tacrolimus, for 24 h and 48 h treatment periods. Tacrolimus induced CA and MN frequency at all concentrations for 24 and 48 h In additon, it induced the SCE at the highest concantration for 24 h and at 25 and 100 ng/mL for 48 h. Tacrolimus decreased MI at all concentrations (except 5 ng/mL) for all treatment periods. It also inhibited the RI at 50 and 100 ng/mL concentrations for 24 h and at all concentrations for 48 h. Treatments given with tacrolimus result in the enhance of the different endpoints ofgenotoxicity, suggesting its mutagenic action on lymphocytes in vitro.


PMID: 22946334

DOI: 10.1134/s1022795412050134

Other references

Zong-Yi, W.; Wang Sung., 1986: Distribution and recent status of the Felidae in China. Unknown

Subrahmanyam, A.; Ramesh, T.P.; Ramakrishnan, N., 2007: Oxygenation of human blood using photocatalytic reaction. This report, as a proof of concept, presents the results of oxygenation of human blood using photocatalytic reaction (at lambda = 352 nm) involving the semiconductor thin film junction: tin doped indium oxide (ITO)/nano titanium oxide (TiO2 in ana...

Robert, N.M.; Miyamoto, Y.; Taniguchi, H.; Viger, R.S., 2006: LRH-1/NR5A2 cooperates with GATA factors to regulate inhibin alpha-subunit promoter activity. Inhibin a is the common subunit of the dimeric inhibin proteins known for their role in suppressing pituitary FSH secretion. In this study, we have examined the role of GATA factors and the nuclear receptor, LRH-1/NR5A2, in the regulation of inhib...

Guzzi, R.; Ballista, G.; D.N.colantonio, W.; Carboni, E., 2001: Aerosol maps from GOME data. In this paper, we present a methodology to calibrate the surface reflectance seen by satellite and validate the aerosol optical properties retrieved by the GOME instrument. Data are also visualized in maps by a tool properly developed, named GOMEV...

Manisha DebMandal; Shyamapada Mandal; Nishith Kumar Pal, 2012: Detection of intestinal colonization of probiotic Lactobacillus rhamnosus by stool culture in modified selective media. To develop an approach for making a selective and differential media specific for <italic>Lactobacillus rhamnosus</italic> Goldin and Gorbach (LGG) in order to distinguish it from other bacterial flora in fecal samples. The current med...

Lyle, J.M.; Ford, W.B., 1993: Review of trawl research 1979-1987, with summaries of biological information for the major species. Tasmania Department of Sea Fisheries Marine Laboratories Technical Report, 46: 1-169

Anonymous, 2007: PHO, hospital track key indicators to attract P4P dollars in cap contracts. Capitation Rates & Data 12(9): 97-102

Stern, J.A.; Tobias, J.R.; Keene, B.W., 2012: Complete atrioventricular block secondary to cardiac lymphoma in a dog. Third degree atrioventricular (AV) block was observed in a patient with a roughly spherical mass measuring approximately 1 1 1 cm, visible in the basilar portion of the interventricular septum on 2-dimensional transthoracic echocardiographic exami...

Cernusco, L.R., 1999: Apparatus for use in picking fruit. Official Gazette of the United States Patent & Trademark Office Patents 1220(2): 1068, March 9

Stewart, J.M., 2015: A multi-level approach for promoting HIV testing within African American church settings. The African American church is a community-based organization that is integral to the lives, beliefs, and behaviors of the African American community. Engaging this vital institution as a primary setting for HIV testing and referral would signific...