+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Microfabricated devices for biomolecule encapsulation

, : Microfabricated devices for biomolecule encapsulation. Electrophoresis 33(17): 2639-2649

Biomolecule encapsulation in droplets is important for miniaturizing biological assays to reduce reagent consumption, cost and time of analysis, and can be most effectively achieved by using microfabricated devices. Microfabricated fluidic devices can generate emulsified drops of uniform size with controlled dimensions and contents. Biological and chemical components such as cells, microgels, beads, hydrogel precursors, polymer initiators, and other droplets can be encapsulated within these drops. Encapsulated emulsions are appealing for a variety of applications since drops can be used as tiny reaction vessels to perform high-throughput reactions at fast rates, consuming minimal sample and solvent amounts due to the small size (micron diameters) of the emulsion drops. Facile mixing and droplet coalescence allow for a diversity of assays to be performed on-chip with tunable parameters. The simplicity of operation and speed of analysis with microencapsulated drops lends itself well to an array of quantitative biomolecular studies such as directed evolution, single-molecule DNA amplification, single-cell encapsulation, high-throughput sequencing, enzyme kinetics, and microfluidic cell culture. This review highlights recent advances in the field of microfabricated encapsulating devices, emphasizing the development of emulsifying encapsulations, device design, and current assays that are performed using encapsulating droplets.


PMID: 22965707

DOI: 10.1002/elps.201200189

Other references

Plavcan J.M.; Gomez A.M., 1990: Phyletic dwarfing and dental scaling in callitrichines. American Journal of Physical Anthropology 81(2): 282

McAllister, M.; Levett-Jones, T.; Petrini, M.A.; Lasater, K., 2016: The viewing room: A lens for developing ethical comportment. Healthcare is dynamic and complex, and against this background, nursing students must negotiate the transition from lay person to healthcare professional. Diverse life experiences and learning styles can further complicate this journey of transfor...

Kent, S.; Alewood, P., 2007: Special issue - Tribute to Bruce Merrifield. International Journal of Peptide Research and Therapeutics 13(1-2): 29

Odouard, A., 1972: Real estate and speculative agriculture in the Canary Islands. The concentration of ownership of land on a speculative basis in the main irrigated zone of the Canary Islands is commented on. Absentee landlords form the main category of landowners. There is a great deal of contrast between high income earning...

Shaw, J.J.; Wong, L.L.; Graff, A.H.; Olaya, H.; Barcelon, S.A.; Degady, M.; Brine, C.J., 1994: Chewing gum containing wheat gluten. Official Gazette of the United States Patent & Trademark Office Patents 1168(4): 2505, Nov 22

Polacheck, W.J.; Charest, J.L.; Kamm, R.D., 2011: Interstitial flow influences direction of tumor cell migration through competing mechanisms. Interstitial flow is the convective transport of fluid through tissue extracellular matrix. This creeping fluid flow has been shown to affect the morphology and migration of cells such as fibroblasts, cancer cells, endothelial cells, and mesenchym...

Patzer, H.; Ballnus, U.; Ballnus, J., 1982: Early infantile autism (Kanner) II. Kinderarztliche Praxis 50(7): 333-345

Ben, A.Fo., 2017: Carphology. Practical Neurology 17(1): 81-81

Kisslo J.A.; Von Ramm O.T.; Behar V.S., 1978: Echo cardiographic evaluation of ischemia descriptors of the ventricular short axis. Circulation Supplement 58(2): II-40

Nosaka, N.; Goda, Y.; Knaup, E.; Tsukahara, K.; Yumoto, T.; Ugawa, T.; Ujike, Y., 2016: Characteristics and Costs of Ladder Fall Injuries: A Report from a Single Emergency Center in Okayama. We sought to identify the incidence, injury patterns, and financial burden of ladder fall injuries to provide a reference for reinforcing guidelines on the prevention of such injuries. We enrolled the patients who were injured in a ladder-related...