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Identification of novel glycolipid ligands activating a sulfatide reactive, CD1d-restricted, type II natural killer T lymphocyte


, : Identification of novel glycolipid ligands activating a sulfatide reactive, CD1d-restricted, type II natural killer T lymphocyte.

Sulfatide-reactive CD1d-restricted natural killer T (NKT) lymphocytes belong to the type II NKT cell subset with diverse TCRs, and have been found to regulate experimental autoimmune encephalomyelitis, tumor immunity and experimental hepatitis in murine models. NKT cells can be activated by self-lipids presented by CD1d, manifested as autoreactivity. The identity of most of these self-lipids remains unknown. By isolating lipids from a CD1d expressing, highly stimulatory antigen presenting cell, we identified isoforms of ?-glucosylceramide (GlcCer), with sphingosine and fatty acid chain lengths of C24: and C16:, that activated a sulfatide-reactive type II NKT cell hybridoma. A screen of structurally related glycosphingolipids demonstrated ?-galactosylceramide (GalCer) as another ligand, and further, that the lysoforms were the most potent isoform of the glycosphingolipid ligands, followed by isoforms with a long fatty acid chain of C24. Thus, the same type II NKT cell was activated by several ligands, namely sulfatide, GlcCer and GalCer. However, CD1d-dependent reactivity to antigen presenting cells lacking all GlcCer-based glycosphingolipids, or all glycosphingolipids, was maintained. This suggests that other endogenous, non-glycosphingolipid, lipid ligands contribute to steady state autoreactivity by type II NKT cells.

US$19.90

DOI: 10.1002/eji.201142350


Other references

Anonymous, 2012: Progress in the research of fossil symphyta in hymenoptera insecta. This paper reviews the history and current status of research work on fossil Symphyta, a suborder of Hymenoptera. A list summarizing all described fossil symphytan species, with their distributions and geological ages is provided. Trend of the div...

Simon, H.U.; Lauterbach, H.; Börner, A.; Wachtel, S., 1990: Diacetyl-splenopentin (BCH 069) did not affect the concentrations of various hormones in men. We investigated the effect of BCH 069 (similar structure as thymopentin) on concentrations of hormones of the hypophysis, adrenals, gonads and thyroid glands in men after a therapy period of 4 or 6 weeks. No significant effects of BCH 069 three ti...

Gutheil, H.; Neumann, W., 1966: On the repeated familial occurrence of patent ductus arteriosus Botalli. Archiv für Kinderheilkunde 175(1): 69-75

Sarg, T.M.; Ateya, A.M.; Farrag, N.M.; Abbas, F.A., 1991: Constituents and biological activity of Bidens pilosa L. grown in Egypt. Column chromotography and preparative TLC of the light petroleum-diethyl ether extract from Bidens pilosa L. afforded tridecapentyn-1-ene, trideca-2,12-diene-4,6,8,10-tetrayne-1-ol, trideca-3,11-diene-5,7,9-triyne-1,2-diol and trideca-5-ene-7,9,11...

Welles, G.W.H.; Buitelaar, K., 1989: Tomatoes. Extensive research on effect of plant quality. Recent research on the relationship between the plant quality (especially plant size) at planting and the yields of glasshouse tomatoes is discussed and reviewed. Data are graphically presented from a substrate trial on the cropping of beefsteak a...

Chen Ruilu.; Bao Xiangzhi.; Wang Suyun.; Sun Yajie.; L.L.qun.; Liu Jirong., 1988: A radar equipment for entomological observation and its test. Acta Ecologica Sinica, 82: 176-182

Shearer J.C.; Clarkson B.R., 1998: Whangamarino wetland; effects of lowered river levels on peat and vegetation. International Peat Journal 8: 52-65

Petit, L.Franck.; Jalabert, M.; Buhler, E.; Malvache, A.; Peret, Aélique.; Chauvin, Y.; Watrin, Fçoise.; Represa, A.; Manent, J-Bernard., 2014: Normotopic cortex is the major contributor to epilepsy in experimental double cortex. Subcortical band heterotopia (SBH) is a cortical malformation formed when neocortical neurons prematurely stop their migration in the white matter, forming a heterotopic band below the normotopic cortex, and is generally associated with intractabl...

Edwards, L.F., 1954: Cincinnati's Old Cunny, a notorious purveyor of human flesh. Ohio State Medical Journal 50(5): 466-469

Wüst, R.C.I.; Degens, H.; Jones, D.A., 2008: Muscle function in smokers: clearing up the smoke. Chest 134(1): 219-20; Author Reply 220