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Treatment of femoral head osteonecrosis with advanced cell therapy in sheep

, : Treatment of femoral head osteonecrosis with advanced cell therapy in sheep. Archives of Orthopaedic and Trauma Surgery 132(11): 1611-1618

The purpose of this study was to evaluate the efficacy of core decompression associated with advanced cell therapy for the treatment of femoral head osteonecrosis in an established sheep model. Early stage osteonecrosis of the right hip was induced cryogenically in 15 mature sheep. At 6 weeks, the sheep were divided into three groups, Group A: core decompression only; Group B: core decompression followed by implantation of an acellular bone matrix scaffold; Group C: core decompression followed by implantation of a cultured BMSC loaded bone matrix scaffold. At 12 weeks, MRI hip studies were performed and then the proximal femur was harvested for histological analysis. In the group of advanced cell therapy, Group C, there was a tendency to higher values of the relative surface of newly formed bone with a mean of 2.3 versus 11.27 % in Group A and 13.4 % in Group B but it was not statistically significant. However, the mean relative volume of immature osteoid was 8.6 % in Group A, 14.97 in Group B, and 53.49 % in Group C (p < .5), revealing a greater capacity of osteoid production in the sheep treated with BMSCs. MRI findings were not conclusive due to constant bone edema artifact in all cases. Our findings indicate that a BMCSs loaded bone matrix scaffold is capable of stimulating bone regeneration more effectively than isolated core decompression or in association with an acellular scaffold in a preclinical femoral head osteonecrosis model in sheep.


PMID: 22821379

DOI: 10.1007/s00402-012-1584-6

Other references

Van Soest, W.; D.M.ester Manger Cats, V., 1956: Does the aphid Myzus persicae imbibe tobacco mosaic virus?. In order to investigate whether Myzus persicae were feeding, takes up tobacco mosaic virus, droplets emerging from stylets cut while aphids were feeding, were tested for the virus by: inoculation of Nicotiana rustica and N. tabacum var. White Burl...

Gallay P.; Cyteval A.; Grolleau R.; Puech P., 1990: Atrial common flutter is the whole atrium necessary to circus movement. European Heart Journal 11(ABSTR SUPPL): 6

Carlson, R.L.; Armelagos, G.J., 1965: Cradleboard Hoods, Not Corsets. Utmost caution should be exercised in interpreting archeological data out of context. Certain artifacts described in the literature on human paleopathology as therapeutic corsets are in fact hoods for cradleboards. Close cooperation among speciali...

Mogha I.V.; Singh G.R., 1984: Effect of cecal resection on urea total volatile fatty acids acid base and gas tension in sheep. Indian Journal of Animal Sciences 54(10): 1008-1009

Cherian, M.N.; Mathews, M.P., 1997: Use of a J-wire cover as an endotracheal tube changer. Journal of Clinical Anesthesia 9(7): 586-588

Robinson, R.W.; Puchalski, J.T., 1980: Systematics of the melon-squash. The "melon-squash" with large (18 lb) variable fruits, similar to those of Cucurbita moschata 'Golden Cushaw', and previously described as C. maxima, has proved to be a very variable large-fruited type of C. moschata. Fruit was...

Depres J P.; Greene A.E.; Crabbe P., 1981: Synthesis of ring modified prostaglandins. The total syntheses of the ring modified prostaglandins [PG] [e.g., 11-nor PGF2.alpha., 11-nor PGE2, 8-aza-11-nor PGE2, ID-aza-11-nor PGE2] are described.

Yoon, G.S.; Yu, E., 2001: Overexpression of promyelocytic leukemia protein and alteration of PML nuclear bodies in early stage of hepatocarcinogenesis. Promyelocytic leukemia protein (PML) is a major component of PML nuclear bodies (PML NBs). Fusion of promyelocytic leukemia gene (PML) with retinoic acid receptor alpha gene with the t (15;17) translocation causes disassembly of PML NBs, leading t...

Syahril, N.A.; Mariana, N.S.; Sidhu, H.S.; Rosli, R., 2002: In vitro expression of the ctxB toxin gene towards the development of a DNA vaccine against cholera. The complete eradication of cholera is an unachievable goal because it is now firmly established that there are environmental reservoirs for Vibrio cholerae. Although there are effective treatments for this disease, they are expensive and impracti...

Larmi, T.K.; Fock, G., 1958: Cystic duct remnant; a cause of biliary distress following incomplete cholecystectomy. Acta Chirurgica Scandinavica 114(5): 361-378