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Single-step reconstitution of apo-hemoproteins at the disruption stage of Escherichia coli cells

, : Single-step reconstitution of apo-hemoproteins at the disruption stage of Escherichia coli cells. Chembiochem 13(14): 2045-2047

Hemoproteins on their metal: We report a novel strategy for the reconstitution of hemoproteins with non-natural metal complexes; simple addition of manganese and ruthenium porphyrin to E. coli cells immediately prior to homogenization yields the reconstituted proteins. We believe that this simple approach could become a standard reconstitution method for hemoproteins.


PMID: 22851307

DOI: 10.1002/cbic.201200446

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