+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Development and Characterization of Genomic and Gene-Based Microsatellite Markers in North American Red Oak Species

, : Development and Characterization of Genomic and Gene-Based Microsatellite Markers in North American Red Oak Species. Plant Molecular Biology Reporter 31(1): 231-239

Oaks (Quercus: Fagaceae) are ecological and economic keystones of many forested ecosystems but effective genetic management strategies are hindered by high levels of phenotypic plasticity within species and frequent hybridization among them. These same features, however, make oak communities suited for the study of speciation, hybridization, and genetic adaptation. Efforts to develop new and to adapt existing genomic resources to less-studied members of this genus should not only improve oak conservation and management but also aid the study of fundamental evolutionary processes. Here, we present a suite of 27 highly polymorphic simple sequence repeat (SSR) markers tested in four North American red oak (Quercus section Lobatae) species: Q. rubra, Q. ellipsoidalis, Q. coccinea, and Q. velutina. Five markers are genomic SSRs (gSSRs) four novel and one previously transferred from Q. petraea and 22 are gene-based SSRs derived from Q. robur and Q. petraea expressed sequence tags (EST-SSRs). Overall, levels of polymorphism detected with these primer pairs were high, with gene diversity (He) averaging 0.66 across all loci in natural populations. In addition, we show that EST-SSR markers may have the potential to detect divergent selection at stress-resistance candidate genes among closely related oak species.


DOI: 10.1007/s11105-012-0495-6

Other references

Krishan Chandra; Karmakar, J.B.; Singh, S.P., 1995: Effect of single strain versus multistrain inoculation soybean (Glycine max) and pea (Pisum sativum) crops in acidic soils. Experimental results showed that multi-strain inoculants were as good as the most effective single strain inoculants. The percentage of nodules occupied, nodule DW, plant DW, and grain yield per plant using the multi-strain inoculant were highly p...

Taylor, J.K.; Zhang, Q.Q.; Burkin, T.; Cooper, S.; Wyatt, J.; Freier, S.; Dean, N.M., 1999: Upregulation of Bcl-xs through modulation of Bcl-x splicing by chemically modified antisense oligonucleotides. Proceedings of the American Association for Cancer Research Annual Meeting 40: 217-218, March

Hmamouchi, I.; Costes, Vérie.; Combe, B.; Morel, J., 2010: Scurvy as the presenting illness of Whipple's disease exacerbated by treatment with etanercept in a patient with ankylosing spondylitis. Journal of Rheumatology 37(5): 1077-1078

Sandberg, L.E.; Zilstorff, P.Dersen, K., 1961: Directional preponderance in temporal lobe disease. Archives of Otolaryngology 73: 139-144

Coles, G.C., 1966: Studies on resilin biosynthesis. The cells that deposit resilin in Schistocerca gregaria have been studied to test the hypothesis that this protein is formed by the cross-linking of proresilin by a peroxidase enzyme. A peroxidase, resembling that from horse-radish, is present in...

Mirsaev, T.R., 2016: Clinical characteristics of syringomyelia in the Bashkir disease cluster. Цель исследования — анализ клинических проявлений сирингомиелии на материале Башкирского эпидемиологического кластера. Материал и ...

Chinnayelka, S.; McShane, M.J., 2005: Microcapsule biosensors using competitive binding resonance energy transfer assays based on apoenzymes. This paper reports the first demonstration of a fluorescence resonance energy transfer based glucose sensor, wherein a competitive binding (CB) assay is encapsulated into polyelectrolyte microcapsules. The work supports the concept that microcapsu...

Ishchanov, M., 1981: Economic and geographical problems in the development of irrigated farming in the Amu-Darya valley. After an introductory description of natural conditions in the 244 000 kmsuperscript 2 Amu-Darya valley in the Uzbek SSR and Turkmen SSR, the study describes the present state of irrigated farming in the area. It goes into detail on questions of w...

Ulgen, M.; Ozer, U.; Kucukguzel, I.; Gorrod, J.W., 1997: Microsomal metabolism of N-benzyl-N-ethylaniline and N-benzyl-N-ethyl-p-toluidine. The in vitro hepatic microsomal metabolism of two tertiary anilines, N-benzyl-N-ethylaniline (NBNEA) and N-benzyl-N-ethyl-p-toluidine (NBNEPT), was examined in order to determine whether these compounds produce amide derivatives (benzoyl or acetyl...

Zhang Bin, 1984: Method for altering the initial velocity V (sub o) of continuous medium. Shiyou Wu-Tan = Geophysical Prospecting for Petroleum 23(4): 92-98