geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Dual lineage-specific expression of Sox17 during mouse embryogenesis


, : Dual lineage-specific expression of Sox17 during mouse embryogenesis. Stem Cells 30(10): 2297-2308

Sox17 is essential for both endoderm development and fetal hematopoietic stem cell (HSC) maintenance. While endoderm-derived organs are well known to originate from Sox17-expressing cells, it is less certain whether fetal HSCs also originate from Sox17-expressing cells. By generating a Sox17(GFPCre) allele and using it to assess the fate of Sox17-expressing cells during embryogenesis, we confirmed that both endodermal and a part of definitive hematopoietic cells are derived from Sox17-positive cells. Prior to E9.5, the expression of Sox17 is restricted to the endoderm lineage. However, at E9.5 Sox17 is expressed in the endothelial cells (ECs) at the para-aortic splanchnopleural region that contribute to the formation of HSCs at a later stage. The identification of two distinct progenitor cell populations that express Sox17 at E9.5 was confirmed using fluorescence-activated cell sorting together with RNA-Seq to determine the gene expression profiles of the two cell populations. Interestingly, this analysis revealed differences in the RNA processing of the Sox17 mRNA during embryogenesis. Taken together, these results indicate that Sox17 is expressed in progenitor cells derived from two different germ layers, further demonstrating the complex expression pattern of this gene and suggesting caution when using Sox17 as a lineage-specific marker.

US$19.90

PMID: 22865702

DOI: 10.1002/stem.1192


Other references

Ivanenko, VN., 1997: Redescription of Asterocheres simulans (Copepoda, Siphonostomatoida, Asterocheridae) - a symbiont of Suberites domuncula ficus (Spongia) from the White Sea. Comments of the taxonomy and ecology. The cyclopiform copepod Asterocheres simulans (Th. Scott, 1898) (Siphonostomatoida, Asterocheridae) described from the North Sea was found associated with the sponge Suberites domuncula ficus (Johnston, 1842) (Demospongiae, Tetractinomorpha, Hadro...

Derrieu, G.; Pougnas, J.L.c; Monginoux, P.; Karst, C., 2002: Compositions which contain at least one cationic polymer and at least one active molecule contained in at least one micro or nonopartculate vector, and their use for treating living or inert surfaces. The present invention relates to compositions which contain at least one cationic polymer and at least one active molecule contained in at least one micro or nanoparticulate vector, and their use for treating living or inert surfaces.

Weiss Charles A.J.; Malone Philip G.; Hock Vincent F.; McInerney Michael K.; Morefield Sean W., 2005: Use of small-scale electro-osmotic systems in controlling groundwater movement around structures. Small-scale electro-osmotic systems for use around commercial and residential structures are becoming increasingly common as a method of controlling the water seeping through concrete foundations or seeping into basements and other underground str...

Boxer, R.S.; Dolansky, M.A.; Frantz, M.A.; Prosser, R.; Hitch, J.A.; Piña, I.L., 2012: The Bridge Project: improving heart failure care in skilled nursing facilities. Rehospitalization rates and transitions of care for patients with heart failure (HF) continue to be of prominent importance for hospital systems around the United States. Skilled nursing facilities (SNF) are pivotal sites for transition especially...

Anonymous, 1961: Preliminary Mohole Project drilling successful. "New vistas in geologic knowledge and petroleum exploration came sharply into focus as the CUSS 1 completed the first phase of the Mohole Project experimental drilling near Guadalupe Island off the coast of Mexico. The specially designed diam...

Coles, T.R.; John, N.W.; Sofia, G.; Gould, D.A.; Caldwell, D.G., 2011: Modification of commercial force feedback hardware for needle insertion simulation. A SensAble Omni force feedback device has been modified to increase the face validity of a needle insertion simulation. The new end effector uses a real needle hub and shortened needle shaft in place of the Omni's pre-fitted pen shaped end ef...

Vorob'ev, V.F., 1966: On the method of breeding composite populations of spring wheat. The promising composite populations Southeastern 144, South-eastern 155, South-eastern 9, Graecum 310 and Albidum have been bred. Data on the fertility of the synthetics are cited. Populations composed of lines differing in response to external fa...

Chick, J.; Lehert, P.; Landron, F., 2002: Does acamprosate improve control of drinking as well as aiding abstinence?. Alcoholism Clinical & Experimental Research 26(5 Supplement): 84A, May

Fandeev, B., 1964: Ayrshire cattle in Finland. There have been several importations of Ayrshires to Finland since the first in 1845; there are now 400, 000 purebred and crossbred cows, representing about 36% of the total cows. The cattle are described. In 1961-62 the production of recorded cow...

Bolf, J.; Kubacek, L.; Martiny, S.; Siska, K.; Hubka, M., 1965: Experimental methods for the study of fluid flow curves. Eksperimental'naia Khirurgiia i Anesteziologiia 10(1): 3-11